early detection of compromised circulation is essential for postoperative monitoring of free flap. Hourly clinical checkups such as inspection and palpation still result in a delay in detection. Conversely, optical reflection and temperature measurement are useful alternatives for detecting blood circulation. However, conventional methods that verify ischemia and congestion within a short period have not been reported. In this study, we measured short-term changes in optical reflection and temperature in a rat flap using a wearable flexible sensor probe previously developed in our laboratory. Five ischemia and five congestion groin flap models were measured using a sensor probe and reference devices. Curve fitting was performed on transition signals to evaluate changes in signals and their time constants. The optical reflection signal decreased after venous ligation and increased after arterial ligation. The parameters of the fitted curves indicate a significant difference between congestion and ischemia at p < 0.01 (probability value), which was detected within a few minutes after ligation. However, insufficient significance was observed in the temperature signal. Our method gives supporting information to verify ischemia and congestion, and has the potential to rapidly detect compromised circulation. Tissue transplantation involves surgery to recover tissue defects after cancer excision or injury. Tissue including vasculature is lifted from its original healthy body location and transplanted to the defect location. The tissue, termed a free flap, receives blood supply by anastomosis of arteries and veins at the defect location, restoring circulation to the free flap. As the expected quality of life of patients has been increasing recently, tissue transplantation is more commonly performed in hospitals. However, although the technique has advanced, the risk of flap failure remains 1-9%, depending on the position of the reconstruction 1,2,3,4,5. The key reason for flap failure is compromised circulation in the blood vessels due to thrombosis 1,6,7 , which occurs in one week after the surgery in most cases. Early salvage surgery soon after the compromised circulation event is essential to increase the rescue rate of tissue 8,9. Thus, real time detection of tissue circulation is necessary. Regular postoperative checkups in hospital involve manual checkups such as pin prick tests, inspection, and palpation. Pin prick tests monitor blood circulation by examining bleeding from the skin surface. Slow or absent bleeding occurs with arterial occlusion (ischemia), whereas darker bleeding is observed with venous occlusion (congestion). During inspection, a pale flap colour indicates an ischemia event, whereas a cyanotic purple colour represents a congestion event. During palpation, a decrease in skin temperature may suggest ischemia, but temperature change is not significant during congestion 10. Some groups have reported that hourly checkups of transplanted tissue are required during the first two postoperative days 7,11,...