Current Technologies and Related Issues for Mushroom Transformation

Kim, Sinil; Ha, Byeongsuk; Ro, Hyeon-Su

doi:10.5941/myco.2015.43.1.1

Cited by 22 publications

(16 citation statements)

References 88 publications

(114 reference statements)

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“…4). Binary vector pBHg-BCA1, the promoter of glyceraldehyde-3-phosphate dehydrogenase with the first exon and intron (Pgpd) (33) and the terminator of trpC (TtrpC) (34,35) were used to construct pdd1-OE and pdd1-RNAi. The pdd1 overexpression vector was completed by introducing a copy of pdd1 flanked by Pgpd and TtrpC (Fig.…”

Section: Resultsmentioning

confidence: 99%

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A Single Transcription Factor (PDD1) Determines Development and Yield of Winter Mushroom ( Flammulina velutipes )

Xie

et al. 2019

Appl Environ Microbiol

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Most of the edible mushrooms cannot be cultivated or have low yield under industrial conditions, partially due to the lack of knowledge on how basidioma (fruiting body) development is regulated. From winter mushroom (Flammulina velutipes), one of the most popular industrially cultivated mushrooms, a transcription factor, PDD1, with a high-mobility group (HMG)-box domain was identified based on its increased transcription during basidioma development. pdd1 knockdown by RNA interference affected vegetative growth and dramatically impaired basidioma development. A strain with an 89.9% reduction in the level of pdd1 transcription failed to produce primordia, while overexpression of pdd1 promoted basidioma development. When the transcriptional level of pdd1 was increased to 5 times the base level, the mushroom cultivation time was shortened by 9.8% and the yield was increased by at least 33%. RNA sequencing (RNA-seq) analysis revealed that pdd1 knockdown downregulated 331 genes and upregulated 463 genes. PDD1 positively regulated several genes related to fruiting, including 6 pheromone receptor-encoding genes, 3 jacalin-related lectin-encoding genes, FVFD16, and 2 FVFD16 homolog-encoding genes. PDD1 is a novel transcription factor with regulatory function in basidioma development found in industrially cultivated mushrooms. Since its orthologs are widely present in fungal species of the Basidiomycota phylum, PDD1 might have important application prospects in mushroom breeding. IMPORTANCE Mushrooms are sources of food and medicine and provide abundant nutrients and bioactive compounds. However, most of the edible mushrooms cannot be cultivated commercially due to the limited understanding of basidioma development. From winter mushroom (Flammulina velutipes; also known as Enokitake), one of the most commonly cultivated mushrooms, we identified a novel transcription factor, PDD1, positively regulating basidioma development. PDD1 increases expression during basidioma development. Artificially increasing its expression promoted basidioma formation and dramatically increased mushroom yield, while reducing its expression dramatically impaired its development. In its PDD1 overexpression mutants, mushroom number, height, yield, and biological efficiency were significantly increased. PDD1 regulates the expression of some genes that are important in or related to basidioma development. PDD1 is the first identified transcription factor with defined functions in mushroom development among commercially cultivated mushroom species, and it might be useful in mushroom breeding.

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Section: Resultsmentioning

confidence: 99%

“…4A and B, respectively. In both plasmids, the promoter of glyceraldehyde-3-phosphate dehydrogenase-encoding gene gpd (Pgpd) and the terminator of indole-3-glycerol phosphate synthase-encoding gene trpC (TtrpC) were used to control expression of pdd1 or the sense and antisense sequences of pdd1 (33)(34)(35).…”

Section: Methodsmentioning

confidence: 99%

A Single Transcription Factor (PDD1) Determines Development and Yield of Winter Mushroom ( Flammulina velutipes )

Xie

et al. 2019

Appl Environ Microbiol

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“…The binary vector pBHg-BCA1 containing the resistance gene of hygromycin was used in the construction of the Fvcpc2 overexpression plasmid and RNAi plasmid as previously described (Wu et al, 2019). The promoter of glyceraldehyde-3-phosphate dehydrogenase Pgpd and terminator of trpC (TtrpC) were used to control the expression of Fvcpc2 or the sense and antisense sequence of Fvcpc2 in both plasmids (Kuo et al, 2004;Sekiya et al, 2013;Kim et al, 2015).…”

Section: Plasmid Construction and Transformation In F Velutipesmentioning

confidence: 99%

“…To understand the function of Fvcpc2 in F. velutipes, the Fvcpc2 overexpression vector Fvcpc2-OE and the Fvcpc2 knockdown vector Fvcpc2-RNAi were constructed, respectively. The binary vector pBHg-BCA1, the promoter of glyceraldehyde-3-phosphate dehydrogenase (Pgpd) (Kuo et al, 2004) and the terminator of trpC (TtrpC) (Sekiya et al, 2013;Kim et al, 2015) were used to construct these plasmids as previously described (Wu et al, 2019).…”

Section: Generation Of Mutants For Fvcpc2 Overexpression and Knockdowmentioning

confidence: 99%

A WD40 Protein Encoding Gene Fvcpc2 Positively Regulates Mushroom Development and Yield in Flammulina velutipes

Zhang

et al. 2020

Front. Microbiol.

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“…Because of the high regeneration efficiency and easy preparation, mycelia were used in this study. Successful ATMT of filamentous fungi relies on several factors, such as acetosyringone (AS) concentration, strains of A. tumefaciens , cultivation conditions, recipient tissue, and so on [30].…”

Section: Introductionmentioning

confidence: 99%

Effects of Medium Composition and Genetic Background on Agrobacterium-Mediated Transformation Efficiency of Lentinula edodes

Yan

Zhou

et al. 2019

Genes

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The establishment of genetic transformation method is crucial for the functional genomics research in filamentous fungi. Although the transformation method has been developed in several types of fungi, a highly efficient and convenient transformation system is desperately needed in Lentinula edodes. Present work established the Agrobacterium-mediated transformation (ATMT) of basidiomycete L. edodes in both monokaryon and dikaryon mycelia by using constructed binary plasmid pCAMBIA-1300-GFP. Then, the transformation efficiency of ATMT was evaluated by using different mediums for recipient incubation and different varieties of L. edodes. The results showed that in dikaryon strain W1, the positive hygromycin-resistant transformants was observed in all medium with the positive frequency of selected transformants that ranged from 0 to 30%. While in the monokaryon strain W1-26, only the millet medium group obtained positive transformants with a positive frequency of 75.48%. Moreover, three dikaryotic wild strains (YS55, YS3334, and YS3357) and two dikaryotic cultivated strains (W1 and S606) showed the highest transformation efficiency, with 32.96% of the germination frequency, and 85.12% of positive frequency for hygromycin-resistant transformants. This work demonstrated that Agrobacterium-mediated transformation was successfully performed in L. edodes, and the genotype of recipients as well as the medium for mycelial incubation were suggested to play key roles in determining the transformation efficiency. These findings may provide new avenues for the genetic modification of edible mushroom and may extend the cognition of DNA-mediated transformation in filamentous fungi.

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Current Technologies and Related Issues for Mushroom Transformation

Cited by 22 publications

References 88 publications

A Single Transcription Factor (PDD1) Determines Development and Yield of Winter Mushroom ( Flammulina velutipes )

A Single Transcription Factor (PDD1) Determines Development and Yield of Winter Mushroom ( Flammulina velutipes )

A WD40 Protein Encoding Gene Fvcpc2 Positively Regulates Mushroom Development and Yield in Flammulina velutipes

Effects of Medium Composition and Genetic Background on Agrobacterium-Mediated Transformation Efficiency of Lentinula edodes

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