Current topics of molecular mycobacteriology

Mokrousov, Igor

doi:10.1016/j.meegid.2019.04.027

Cited by 6 publications

(7 citation statements)

References 41 publications

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“…It is generally accepted that methods like spoligotyping or MIRU-VNTR can exclude transmission but are not sufficiently discriminative to confirm transmission chains. This is because genetic markers indicated by each method may show identical results for distinct strains due to convergent evolution and the lack of resolution needed to detect recent transmission [ 9 ]. Transmission chain confirmation is also done through whole-genome sequencing (WGS) and/or epidemiological/contact investigations.…”

Section: Introductionmentioning

confidence: 99%

An overview of tuberculosis outbreaks reported in the years 2011–2020

et al. 2023

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Background In many countries tuberculosis (TB) remains a highly prevalent disease and a major contributor to infectious disease mortality. The fight against TB requires surveillance of the population of strains circulating worldwide and the analysis of the prevalence of certain strains in populations. Nowadays, whole genome sequencing (WGS) allows for accurate tracking of TB transmission. Currently, there is a lack of a comprehensive summary of the characteristics of TB outbreaks. Methods We systematically analyzed studies reporting TB outbreaks worldwide, monitored through WGS of Mycobacterium tuberculosis. We 1) mapped the reported outbreaks from 2011- 2020, 2) estimated the average size of the outbreaks, 3) indicated genetic lineages causing the outbreaks, and 4) determined drug-resistance patterns of M. tuberculosis strains involved in the outbreaks. Results Most data originated from Europe, Asia, and North America. We found that TB outbreaks were reported throughout the globe, on all continents, and in countries with both high and low incidences. The detected outbreaks contained a median of five M. tuberculosis isolates. Most strains causing the outbreaks belonged to lineage four, more rarely to lineage two. Reported outbreak isolates were often drug resistant. Conclusions We conclude that more WGS surveillance of M. tuberculosis outbreaks is needed. Globally standardized procedures might improve the control of M. tuberculosis infections.

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Section: Introductionmentioning

confidence: 99%

An overview of tuberculosis outbreaks reported in the years 2011–2020

et al. 2023

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“…The NATs can be developed to improve the diagnostics of mycobacterial infections (MTBC or nontuberculous mycobacteria) and environmental control [12]. In addition to mycobacterial diagnostics, numerous applications have been developed using nucleic acid detection as the marker of bacterial, viral, or fungal contamination or infection.…”

Section: Natsmentioning

confidence: 99%

“…There are several techniques based on the principle of PCR for the quality control of biologicals. Most of these techniques were developed for the diagnosis of M. tuberculosis and helped reducing the time-to-result, thus allowing faster initiation of appropriate treatments [12,[42][43][44].…”

Section: Pcr Techniquesmentioning

confidence: 99%

Testing of Biological Products: A Review of Alternative Non-microbiological Techniques for Mycobacterial Testing

Marius,

Fernandez

2024

Preprint

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Mycobacteria can be one of the main contaminants of biological products, and their presence can have serious consequences on patients’ health. Thus, specific testing is necessitated by regulations such as the European Pharmacopoeia. The current pharmacopeial reference i.e., microbial culture method cannot ensure an exhaustive detection of mycobacteria due to their growth characteristics. Additionally, the method is time-consuming and requires a continuous supply of culture media, which could be further challenging. Thus, to overcome these challenges, pharmaceutical industries need to consider alternative non-microbiological techniques to detect these fastidious, slow-growing contaminating agents. This review provides an overview of alternative methods, which could be applied within a quality control environment of biological products, and underlines advantages and limitations of these methods. Nucleic acid amplification techniques or direct measurement of mycobacteria stands out as the most suitable alternatives for mycobacterial testing in biological products.

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“…These methods not only avoid lengthy culturing periods and incubations but can also render results in few hours. In addition to the diagnosis of mycobacterial infections (MTBC or nontuberculous mycobacteria), numerous applications have been developed using nucleic acid detection as the marker of bacterial, viral or fungal contamination or infection [14]. These include the detection of non-cultivable microorganisms [15], rapid diagnostics [16], environmental contaminant search [17][18][19] and food safety [20].…”

Section: Overviewmentioning

confidence: 99%

Non-Microbiological Mycobacterial Detection Techniques for Quality Control of Biological Products: A Comprehensive Review

Marius,

Fernandez

2024

Microorganisms

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Mycobacteria can be one of the main contaminants of biological products, and their presence can have serious consequences on patients’ health. For this reason, the European Pharmacopoeia mandates the specific testing of biological products for mycobacteria, a critical regulatory requirement aimed at ensuring the safety of these products before they are released to the market. The current pharmacopeial reference, i.e., microbial culture method, cannot ensure an exhaustive detection of mycobacteria due to their growth characteristics. Additionally, the method is time consuming and requires a continuous supply of culture media, posing logistical challenges. Thus, to overcome these issues, pharmaceutical industries need to consider alternative non-microbiological techniques to detect these fastidious, slow-growing contaminating agents. This review provides an overview of alternative methods, which could be applied within a quality control environment for biological products and underlines their advantages and limitations. Nucleic acid amplification techniques or direct measurement of mycobacteria stand out as the most suitable alternatives for mycobacterial testing in biological products.

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Current topics of molecular mycobacteriology

Cited by 6 publications

References 41 publications

An overview of tuberculosis outbreaks reported in the years 2011–2020

An overview of tuberculosis outbreaks reported in the years 2011–2020

Testing of Biological Products: A Review of Alternative Non-microbiological Techniques for Mycobacterial Testing

Non-Microbiological Mycobacterial Detection Techniques for Quality Control of Biological Products: A Comprehensive Review

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