Purpose: To examine both the distances from the orbital rim to the myoneural junctions (MNJs) and the widths of the MNJs of all extraocular muscles. Methods: Six orbits of 3 post-mortem cadavers were used. The cadavers (1 female and 2 males) were all Japanese with an average age of 76.3 years. The MNJs of the extraocular muscles and their motor nerves were exposed, and then the distance from the orbital rim to each MNJ and the width of each MNJ were examined. Results: The distance from the orbital rim to each MNJ in the 6 extraocular muscles ranged from 24.4 to 33.6 mm and the width of each MNJ ranged from 5.0 to 8.5 mm. Conclusions: It is essential for orbital surgeons to understand both the distance from the orbital rim to MNJs and the widths of MNJs. This information not only aids the understanding of MNJ damage, but also prevents iatrogenic nerve impairment during orbital surgery.