Current Trends in Applications of Circulatory Microchimerism Detection in Transplantation

Andrikovics, Hajnalka; Őrfi, Zoltán; Meggyesi, Nóra; Bors, András; Varga, L; Kövy, Petra; Vilimszky, Zsófia; Kolics, Fanni; Gopcsa, László; Reményi, Péter; Tordai, Attila

doi:10.3390/ijms20184450

Cited by 24 publications

(43 citation statements)

References 117 publications

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“…When the perfusion pump drives liquid flow, turbulence occurs in the drops; cells in the drop settle to the bottom and begin to aggregate. Under different cultural conditions, this chip can be used for various applications such as drug delivery or as an in vitro platform for cell therapy [4,21,64,65]. In addition to the formation and culture of the spheroids, how to observes and analyze the spheroids has been gradually being valued.…”

Section: Discussionmentioning

confidence: 99%

“…Stem cells are cells which have the ability to renew themselves and can differentiate into various cell types-such as blood cells, and cells of the brain, bones, and organs of the human body under certain physiological or experimental conditions-and are widely used in tissue engineering research [3]. After cell passaging and proliferation, these cells are injected back into the patient [4]. However, in both stem cell transplantation and immunotherapy, large numbers of cells are required for preparing a matrix that can be injected back into a patient's body to repair damaged tissue [5].…”

Section: Introductionmentioning

confidence: 99%

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A Dynamic Hanging-Drop System for Mesenchymal Stem Cell Culture

Huang

Tzeng

Chen

et al. 2020

IJMS

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There have been many microfluid technologies combined with hanging-drop for cell culture gotten developed in the past decade. A common problem within these devices is that the cell suspension introduced at the central inlet could cause a number of cells in each microwell to not regularize. Also, the instability of droplets during the spheroid formation remains an unsolved ordeal. In this study, we designed a microfluidic-based hanging-drop culture system with the design of taper-tube that can increase the stability of droplets while enhancing the rate of liquid exchange. A ring is surrounding the taper-tube. The ring can hold the cells to enable us to seed an adequate amount of cells before perfusion. Moreover, during the period of cell culture, the mechanical force around the cell is relatively low to prevent stem cells from differentiate and maintain the phenotype. As a result of our hanging system design, cells are designed to accumulate at the bottom of the droplet. This method enhances convenience for observation activities and analysis of experiments. Thus, this microfluid chip can be used as an in vitro platform representing in vivo physiological conditions, and can be useful in regenerative therapy.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

A Dynamic Hanging-Drop System for Mesenchymal Stem Cell Culture

Huang

Tzeng

Chen

et al. 2020

IJMS

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“…However, other DNA polymorphisms such as variable number of tandem repeats (VNTR), single nucleotide polymorphisms (SNPs), or insertion/deletion polymorphisms (Indel) can be used. The main reason STRs are more suitable for chimerism analysis compared with other polymorphisms is their higher informativity rate [ 31 , 32 , 33 , 34 ]. Nevertheless, a careful selection of STR alleles is required to select the most suitable STR for chimerism monitoring considering all the variables which can affect the analysis [ 18 , 35 , 36 ].…”

Section: Introductionmentioning

confidence: 99%

Chimerism Monitoring Techniques after Hematopoietic Stem Cell Transplantation: An Overview of the Last 15 Years of Innovations

et al. 2021

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Chimerism analysis is a well-established method for monitoring the state of hematopoietic stem cell transplantation (HSCT) over time by analyzing peripheral blood or bone marrow samples of the recipient in several malignant and non-malignant hematologic diseases. From a clinical point of view, a continuous monitoring is fundamental for an effective early therapeutic intervention. This paper provides a comparative overview of the main molecular biology techniques which can be used to study chimerism after bone marrow transplantation, focusing on their advantages and disadvantages. According to the examined literature, short tandem repeats (STR) analysis through simple PCR coupled with capillary electrophoresis (STR-PCR) is the most powerful method which guarantees a high power of differentiation between different individuals. However, other methods such as real-time quantitative PCR (qPCR), digital PCR (dPCR), and next-generation sequencing (NGS) technology were developed to overcome the technical limits of STR-PCR. In particular, these other techniques guarantee a higher sensitivity, which allows for the detection of chimerism at an earlier stage, hence expanding the window for therapeutic intervention. After a comparative evaluation of the various techniques, it seems clear that STR-PCR still remains the gold standard option for chimerism study, even if it is likely that both dPCR and NGS could supplement or even replace the common methods of STR analysis.

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“…This Special Issue contains eight original research studies [20,21,22,23,24,25,26,27], and five review papers [28,29,30,31,32]; the diversity of the papers demonstrates how many different topics are covered by cell-free nucleic acid research.…”

mentioning

confidence: 99%

“…Microchimerism has also recently become a hot topic too. Andrikovics et al discuss microchimerism in the context of various forms of transplantation and transplantation-related advanced therapies, and they show the available cfNA (cell-free nucleic acid) markers and detection platforms [31].…”

mentioning

confidence: 99%