Current trends in high throughput proteomics in cyanobacteria

Ow, Saw Yen; Wright, Phillip C.

doi:10.1016/j.febslet.2009.03.062

Cited by 23 publications

(24 citation statements)

References 82 publications

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“…There are few global proteome studies in cyanobacteria (39, 40, 44–50). In a study on Prochlorococcus marinus MED4 (49), approximately 11% of the theoretical proteome was identified.…”

Section: Resultsmentioning

confidence: 99%

“…Global transcriptomics defined clear differences in the response of Prochlorococcus strain MED4 versus strain MIT9313 to the availability of different N sources (38), and there is some evidence for a response at the protein level (39). Recent advances in high-throughput techniques based on tandem mass spectrometry allow a greater insight concerning the integration, function, and regulation of the proteome (40). Here we used quantitative proteomics to study the effect of the addition of azaserine on the proteome of Prochlorococcus marinus SS120.…”

Section: Introductionmentioning

confidence: 99%

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Quantitative Proteomics Shows Extensive Remodeling Induced by Nitrogen Limitation in Prochlorococcus marinus SS120

et al. 2017

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Prochlorococcus is the most abundant photosynthetic organism on Earth, contributing significantly to global primary production and playing a prominent role in biogeochemical cycles. Here we study the effects of extreme nitrogen limitation, a feature of the oligotrophic oceans inhabited by this organism. Quantitative proteomics allowed an accurate quantification of the Prochlorococcus proteome, finding three main responses to nitrogen limitation: upregulation of nitrogen assimilation-related proteins, including transporters; downregulation of ribosome proteins; and induction of the photosystem II cyclic electron flow. This suggests that nitrogen limitation affects a range of metabolic processes far wider than initially believed, with the ultimate goal of saving nitrogen and maximizing the nitrogen uptake and assimilation capabilities of the cell.

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“…There are few global proteome studies in cyanobacteria (39, 40, 44–50). In a study on Prochlorococcus marinus MED4 (49), approximately 11% of the theoretical proteome was identified.…”

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Quantitative Proteomics Shows Extensive Remodeling Induced by Nitrogen Limitation in Prochlorococcus marinus SS120

et al. 2017

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“…Cyanobacteria may use solar energy as their energy source, CO 2 from the air as their carbon source, and, some of them, atmospheric N 2 as their nitrogen source. With such favorable basic characteristics, well developed molecular tools (3), and existing systems biology analyses (4), cyanobacteria are ideal candidates for a synthetic biological approach.…”

Section: Introductionmentioning

confidence: 99%

Design and characterization of molecular tools for a Synthetic Biology approach towards developing cyanobacterial biotechnology

Huang¹,

Camsund²,

Lindblad³

et al. 2010

Nucleic Acids Research

314

401

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Cyanobacteria are suitable for sustainable, solar-powered biotechnological applications. Synthetic biology connects biology with computational design and an engineering perspective, but requires efficient tools and information about the function of biological parts and systems. To enable the development of cyanobacterial Synthetic Biology, several molecular tools were developed and characterized: (i) a broad-host-range BioBrick shuttle vector, pPMQAK1, was constructed and confirmed to replicate in Escherichia coli and three different cyanobacterial strains. (ii) The fluorescent proteins Cerulean, GFPmut3B and EYFP have been demonstrated to work as reporter proteins in cyanobacteria, in spite of the strong background of photosynthetic pigments. (iii) Several promoters, like PrnpB and variants of PrbcL, and a version of the promoter Ptrc with two operators for enhanced repression, were developed and characterized in Synechocystis sp. strain PCC6803. (iv) It was shown that a system for targeted protein degradation, which is needed to enable dynamic expression studies, is working in Synechocystis sp. strain PCC6803. The pPMQAK1 shuttle vector allows the use of the growing numbers of BioBrick parts in many prokaryotes, and the other tools herein implemented facilitate the development of new parts and systems in cyanobacteria.

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“…Contamination of marine products by algal toxins is a widespread phenomenon, posing significant threats to human and animal health. Proteomics methodologies have been applied to cyanobacteria research more extensively than to other marine microorganisms (Ow & Wright, 2009). Biomarkers in toxin-producers species have been characterized (Chan et al, 2006) but lately, the research is focused to the study of algal toxins response in fish and shellfish by means of protein markers defined by proteomics (Mezhoud et al, 2008;Ronzitti, Milandri, Scortichini, Poletti, &Rossini, 2008 andCastielli, De la Cerda, Navarro, Hervás, &De la Rosa, 2009;Sala et al, 2009).…”

Section: Modificationmentioning

confidence: 99%

The role of proteomics in the study of the influence of climate change on seafood products

Piñeiro¹,

Cañas²,

Carrera³

2010

Food Research International

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a b s t r a c tThe climate change influence over the oceans has been the subject of numerous articles informs and strategies from different scientific perspectives, focused mainly in the ecological impact. The majority of the related studies have been focused in measuring or predicting the physical, chemical, geographical, sociological and economical consequences of this reality, which seems to be unstoppable, and only a few of them are devoted to detect the effects of the climate change over the quality of seafood products, wild or cultivated.The stress produced in marine organisms by the consequences of climate change is reflected at the cell molecular level, being affected the metabolite concentration, the expression of proteins and their modifications. The study of the climate change may take advantage of these molecular changes, which may be used as a source of possible biomarkers of its evolution.After the genomic age, proteomics appears as a young but robust discipline for a global study of the protein content in cells, including their identification, possible modifications, quantification of differential expression and tissue localization, being the most adequate set of methodologies to evidence protein changes in marine organisms affected by climate variations. In the last decade proteomic technologies have experienced an exponential development, but the research has been mainly applied to biomedical and human health research, being scarcely focused to the study of the marine environment. The application of the proteomics methods to study the effects of climate change over seafood, mainly from the safety point of view, is reviewed.

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Current trends in high throughput proteomics in cyanobacteria

Cited by 23 publications

References 82 publications

Quantitative Proteomics Shows Extensive Remodeling Induced by Nitrogen Limitation in Prochlorococcus marinus SS120

Quantitative Proteomics Shows Extensive Remodeling Induced by Nitrogen Limitation in Prochlorococcus marinus SS120

Design and characterization of molecular tools for a Synthetic Biology approach towards developing cyanobacterial biotechnology

The role of proteomics in the study of the influence of climate change on seafood products

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