2013
DOI: 10.1002/mas.21419
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Current trends in mass spectrometry of peptides and proteins: Application to veterinary and sports‐doping control

Abstract: Detection of misuse of peptides and proteins as growth promoters is a major issue for sport and food regulatory agencies. The limitations of current analytical detection strategies for this class of compounds, in combination with their efficacy in growth-promoting effects, make peptide and protein drugs highly susceptible to abuse by either athletes or farmers who seek for products to illicitly enhance muscle growth. Mass spectrometry (MS) for qualitative analysis of peptides and proteins is well-established, … Show more

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Cited by 50 publications
(26 citation statements)
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References 161 publications
(234 reference statements)
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“…22 MS methods for plasma/serum determination of IGF-I has been developed. 22 Anti-doping testing of other illegal exogenous substances including testosterone and erythropoietin has switched to ABP monitoring rather than using population-based cut-off values. 22 Anti-doping testing of other illegal exogenous substances including testosterone and erythropoietin has switched to ABP monitoring rather than using population-based cut-off values.…”
Section: Hours and 3 Hours) After An Injection This Results Is In Agrmentioning
confidence: 99%
“…22 MS methods for plasma/serum determination of IGF-I has been developed. 22 Anti-doping testing of other illegal exogenous substances including testosterone and erythropoietin has switched to ABP monitoring rather than using population-based cut-off values. 22 Anti-doping testing of other illegal exogenous substances including testosterone and erythropoietin has switched to ABP monitoring rather than using population-based cut-off values.…”
Section: Hours and 3 Hours) After An Injection This Results Is In Agrmentioning
confidence: 99%
“…[1][2][3][4] Additionally, growth hormone releasing peptides (GHRPs) trigger the complete growth hormone cascade and thus, all growth-hormone-related activities, accelerating recovery from injuries and stimulating muscle growth. [5][6][7] Gonadotropin-releasing hormone (GnRH), also known as luteinizing hormone-releasing hormone (LH-RH) or gonadorelin, regulates the secretion of gonadotropins. Because of its activity to stimulate gonadotropin and testosterone secretion, GnRH is regarded as a growth promoting agent.…”
Section: Introductionmentioning
confidence: 99%
“…Because of its activity to stimulate gonadotropin and testosterone secretion, GnRH is regarded as a growth promoting agent. 6 Due to their pharmacological effects, the peptides mentioned can be potentially misused in sports to illicitly alter performance. In fact, dermorphin was detected and confirmed in official post-competition equine plasma and urine samples in North America, 8 and GHRP-2 and GHRP-6 were identified in urine samples from human athletes.…”
Section: Introductionmentioning
confidence: 99%
“…1 Currently, 2 different strategies are available for detection of exogenous administration, the first one directly measuring the hormone itself, the second one referring to the GH-responsive biomarkers insulin-like growth factor-I (IGF-I) and procollagen III N-terminal propeptide (P-III-NP). 2,3 A challenge with direct GH-measurement it is that, owing to pulsatile secretion, elevated serum levels are not conclusive as to illicit intake; an aberrant isoform composition of serum GH needs to be detected instead. To this end, with the presently approved 'differential' immunoassay, the amounts of GH measured by two types of antibodies of different specificity are compared: One of them preferentially binds to 22 kDa-GH ('rec GH'), the molecular form evidently used as doping agent; the second one ('pit GH'), in contrast, has been designed to recognize a variety of GH isoforms.…”
Section: Introductionmentioning
confidence: 99%