Current Trends in Rapid Diagnostics for Methicillin-Resistant
            <i>Staphylococcus aureus</i>
            and Glycopeptide-Resistant
            <i>Enterococcus</i>
            Species

Malhotra-Kumar, Surbhi; Haccuria, Kelly; Michiels, M.; Ieven, Margareta; Poyart, Claire; Hryniewicz, Waleria; Goossens, Herman

doi:10.1128/jcm.00326-08

Cited by 105 publications

(96 citation statements)

References 43 publications

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“…Hence, screening for MRSA carriage and contact isolation of MRSA carriers is crucial for effective hospital infection control (3). Employing rapid and sensitive screening assays for MRSA detection could help to further improve infection control, as well as decrease costs (4,7). While traditional culture-based methods tend to be slow and laborious, the next-generation culture methods, such as the use of chromogenic media, which allow a direct colony color-based identification of MRSA from the primary culture, can reduce the time to result from 48 to 18 or 24 h (7).…”

mentioning

confidence: 99%

Impact of a Short Period of Pre-Enrichment on Detection and Bacterial Loads of Methicillin-Resistant Staphylococcus aureus from Screening Specimens

et al. 2009

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We compared the impacts of direct plating on a chromogenic medium and of plating after enrichment (4 h and overnight) on the detection of methicillin-resistant Staphylococcus aureus (MRSA) from 52 patient screening samples. MRSA colony counts for Ϸ70% of samples after overnight pre-enrichment were >20-fold higher than the counts after the other two treatments. The qualitative differences (sample was MRSA positive/ negative) between the results of the direct plating and 4-h pre-enrichment treatments were not significant, indicating no advantage of the latter; however, the number of samples positive for MRSA increased significantly after an overnight sample pre-enrichment (P < 0.005).

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confidence: 99%

Impact of a Short Period of Pre-Enrichment on Detection and Bacterial Loads of Methicillin-Resistant Staphylococcus aureus from Screening Specimens

et al. 2009

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“…We thank Dr. OHara for bringing to attention this important information not available at the time of our initial publication (3). While the previous version of the 3M BacLite rapid MRSA test would fail to detect community-acquired methicillin-resistant Staphylococcus aureus (MRSA) and ciprofloxacin-sensitive hospital-acquired MRSA (3), the new 3M BacLite assay correctly differentiates MRSA and methicillin-sensitive S. aureus (MSSA) strains and detects MRSA from screening samples, as reported recently (1,4).…”

Section: Authors' Replymentioning

confidence: 99%

Novel Rapid Culture-Based Detection Method for Methicillin-Resistant Streptococcus aureus

O’Hara¹

2008

J Clin Microbiol

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In the May 2008 issue of Journal of Clinical Microbiology, provided an excellent minireview of current trends in rapid diagnostics for methicillin-resistant Staphylococcus aureus (MRSA) and glycopeptide-resistant enterococci. In this article, the authors describe a novel rapid culture-based assay, the 3M BacLite rapid MRSA test, which detects MRSA without the need for macroscopic growth of bacterial colonies, allowing the presence or absence of MRSA to be determined within 5 h. The article states the assay detects ciprofloxacin-resistant MRSA and comments that this may lead to the possibility of the assay not detecting communityacquired MRSA strains, as well as some hospital-acquired MRSA strains, that are sensitive to ciprofloxacin. The authors are correct in their statements but refer to an assay formulation that has not been available since February 2007, when ciprofloxacin was replaced by cefoxitin as a selective agent.The ability of the 3M BacLite rapid MRSA test to detect MRSA strains from a diverse genetic background has been evaluated by von Eiff et al. (4). In an extensive study, more than 700 methicillin-susceptible and methicillin-resistant strains, covering Ͼ90% of all registered European MRSA spa types within the SeqNet network, were tested using the 3M BacLite rapid MRSA test. The study reported that all 513 MRSA strains tested were correctly recognized as methicillin resistant while none of the 211 methicillin-susceptible strains were detected as positive. In an additional study (5), 27 MRSA strains recovered from pigs, which have been reported to cause detection problems in some molecular assays, were correctly detected as positive by the 3M BacLite rapid MRSA test. In addition to these analytical studies, there are an increasing number of studies assessing the performance of this assay with clinical specimens. Our group (3) reported a diagnostic sensitivity of 94.6% and a diagnostic specificity of 96.9% for nasal screening swabs and 95.9% sensitivity and 88.8% specificity for groin screening swabs when the assay was referenced to standard operating procedures at three hospital sites in the United Kingdom. More recently Cohen et al. (1) assessed the performance characteristics of the 3M BacLite rapid MRSA assay for a range of body sites compared to chromagar and a commercial molecular assay and concluded that the BacLite assay's performance, ease of use, and capacity best met the needs of the authors' hospital for rapid MRSA screening.The limitations of traditional diagnostic methods coupled with the increase in antimicrobial resistance means that there is a growing demand among health care providers for rapid microbiological tests to assist patient care and disease control. Authors' ReplyWe thank Dr. OHara for bringing to attention this important information not available at the time of our initial publication (3). While the previous version of the 3M BacLite rapid MRSA test would fail to detect community-acquired methicillin-resistant Staphylococcus aureus (MRSA) and ciprofloxacin-sensitive ho...

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“…In general, other highly conserved genes become helpful, which requires additional time and money. In most of the molecular approaches used for identifying Staphylococcus species, 27 genes have been widely used either individually or in combination [1,[17][18][19][20][22][23][24]. Of these 27 genes, only gyrA is among those which are common to all the genomes.…”

Section: Discussionmentioning

confidence: 99%

“…Here, S. aureus ATCC25923 act as a positive control, whereas S. epidermidis ATCC12228 is used as a negative control [5]. Identification of genetically diverse isolates of methicillin-resistant S. aureus (MRSA) has been successfully done using orfX-staphylococcal cassette chromosome mec-(SCCmec) based assays carrying blaZ, ccr, mecA, mecI, and mecR1 genes [1,[22][23][24][25]. A few more genes used for identifying S. epidermidis include: sodA, rpoB, tuf, gap, dnaJ, hsp40, and tRNA intergenic spacer [20].…”

Section: Molecular Assaysmentioning

confidence: 99%

Searching Biomarkers in the Sequenced Genomes of Staphylococcus for their Rapid Identification

et al. 2016

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Bacterial identification using rrs (16S rRNA) gene is widely reported. Bacteria possessing multiple copies of rrs lead to overestimation of its diversity. Staphylococcus genomes carries 5-6 copies of rrs showing high similarity in their nucleotide sequences, which lead to ambiguous results. The genomes of 31 strains of Staphylococcus representing 7 species were searched for the presence of common genes. In silico digestion of 34 common genes using 10 restriction endonucleases (REs) lead to select gene-RE combinations, which could be used as biomarkers. RE digestion of recA allowed unambiguous identification of 13 genomes representing all the 7 species. In addition, a few more genes (argH, argR, cysS, gyrB, purH, and pyrE) and RE combinations permitted further identification of 12 strains. By employing additional RE and genes unique to a particular strain, it was possible to identify the rest 6 Staphylococcus aureus strains. This approach has the potential to be utilized for rapid detection of Staphylococcus strains.

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Current Trends in Rapid Diagnostics for Methicillin-Resistant Staphylococcus aureus and Glycopeptide-Resistant Enterococcus Species

Cited by 105 publications

References 43 publications

Impact of a Short Period of Pre-Enrichment on Detection and Bacterial Loads of Methicillin-Resistant Staphylococcus aureus from Screening Specimens

Impact of a Short Period of Pre-Enrichment on Detection and Bacterial Loads of Methicillin-Resistant Staphylococcus aureus from Screening Specimens

Novel Rapid Culture-Based Detection Method for Methicillin-Resistant Streptococcus aureus

Searching Biomarkers in the Sequenced Genomes of Staphylococcus for their Rapid Identification

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