Current Trends of Microfluidic Single-Cell Technologies

Shinde, Pallavi; Mohan, L.; Kumar, Amogh; Dey, Koyel; Maddi, Anjali; Patananan, Alexander N.; Tseng, Fan‐Gang; Chang, Hwan‐You; Nagai, Masao; Santra, Tuhin Subhra

doi:10.3390/ijms19103143

Cited by 67 publications

(37 citation statements)

References 260 publications

(288 reference statements)

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“…The first routine in clinical practice is the complete blood cells count, determining the frequency of all major blood cells, along with blood biochemistry and molecular markers detection. Thus, microfluidic technologies can be used to obtain a variety of interesting applications, such as PCR amplification and electrophoresis [ 38 , 39 ], immunoassays and flow cytometry [ 40 , 41 ], proteins for analysis via mass spectrometry [ 42 ], DNA analysis [ 43 ], chemical gradient formation [ 44 ], cell manipulation and separation [ 27 , 45 , 46 ], cell patterning [ 47 ] and single-cell analysis [ 25 , 48 , 49 , 50 , 51 , 52 ], NMR [ 53 , 54 , 55 ], electrochemical [ 56 , 57 ] and measurements such as fluid viscosity [ 58 , 59 ], and pH [ 60 , 61 ] of the blood samples and its constituents.…”

Section: Microfluidics Tools: Single-cell Approachmentioning

confidence: 99%

Visualization and Measurements of Blood Cells Flowing in Microfluidic Systems and Blood Rheology: A Personalized Medicine Perspective

Pinho

Carvalho

Gonçalves

et al. 2020

JPM

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Hemorheological alterations in the majority of metabolic diseases are always connected with blood rheology disturbances, such as the increase of blood and plasma viscosity, cell aggregation enhancement, and reduction of the red blood cells (RBCs) deformability. Thus, the visualizations and measurements of blood cells deformability flowing in microfluidic devices (point-of-care devices) can provide vital information to diagnose early symptoms of blood diseases and consequently to be used as a fast clinical tool for early detection of biomarkers. For instance, RBCs rigidity has been correlated with myocardial infarction, diabetes mellitus, hypertension, among other blood diseases. In order to better understand the blood cells behavior in microfluidic devices, rheological properties analysis is gaining interest by the biomedical committee, since it is strongly dependent on the interactions and mechanical cells proprieties. In addition, the development of blood analogue fluids capable of reproducing the rheological properties of blood and mimic the RBCs behavior at in vitro conditions is crucial for the design, performance and optimization of the microfluidic devices frequently used for personalized medicine. By combining the unique features of the hemorheology and microfluidic technology for single-cell analysis, valuable advances in personalized medicine for new treatments and diagnosis approach can be achieved.

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Section: Microfluidics Tools: Single-cell Approachmentioning

confidence: 99%

Visualization and Measurements of Blood Cells Flowing in Microfluidic Systems and Blood Rheology: A Personalized Medicine Perspective

Pinho

Carvalho

Gonçalves

et al. 2020

JPM

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“…In recent years, many microfluidic devices have been developed and applied for the cultivation and analysis at single-cell level under defined environmental conditions. 14–16 Here, cells are trapped in different cultivation chamber geometries and perfused with medium. The cultivation chambers on these microfluidic devices range from 0D to 3D systems.…”

Section: Introductionmentioning

confidence: 99%

dMSCC: A microfluidic platform for microbial single-cell cultivation under dynamic environmental medium conditions

Taeuber

Golze

et al. 2020

Preprint

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In nature and in technical systems, microbial cells are often exposed to rapidly fluctuating environmental conditions. These conditions can vary in quality, e.g., existence of a starvation zone, and quantity, e.g., average residence time in this zone. For strain development and process design, cellular response to such fluctuations needs to be systematically analysed. However, the existing methods for physically emulating rapidly changing environmental conditions are limited in spatio-temporal resolution. Hence, we present a novel microfluidic system for cultivation of single cells and small cell clusters under dynamic environmental conditions (dynamic microfluidic single-cell cultivation (dMSCC)). This system enables to control nutrient availability and composition between two media with second to minute resolution. We validate our technology using the industrially relevant model organism Corynebacterium glutamicum. The organism was exposed to different oscillation frequencies between nutrient excess (feasts) and scarcity (famine). Resulting changes in cellular physiology, such as the colony growth rate and cell morphology were analysed and revealed significant differences with growth rate and cell length between the different conditions. dMSCC also allows to apply defined but randomly changing nutrient conditions, which is important for reproducing more complex conditions from natural habitats and large-scale bioreactors. The presented system lays the foundation for the cultivation of cells under complex changing environmental conditions.

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“…However, conventional in vitro techniques are often insufficient for reconstructing cellular microenvironments in any combination. Single cell manipulation tools [6] are required for mimicking the in vivo environment in vitro with a higher reproducibility.…”

Section: Introductionmentioning

confidence: 99%

Scalable Parallel Manipulation of Single Cells Using Micronozzle Array Integrated with Bidirectional Electrokinetic Pumps

et al. 2020

Self Cite

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High throughput reconstruction of in vivo cellular environments allows for efficient investigation of cellular functions. If one-side-open multi-channel microdevices are integrated with micropumps, the devices will achieve higher throughput in the manipulation of single cells while maintaining flexibility and open accessibility. This paper reports on the integration of a polydimethylsiloxane (PDMS) micronozzle array and bidirectional electrokinetic pumps driven by DC-biased AC voltages. Pt/Ti and indium tin oxide (ITO) electrodes were used to study the effect of DC bias and peak-to-peak voltage and electrodes in a low conductivity isotonic solution. The flow was bidirectionally controlled by changing the DC bias. A pump integrated with a micronozzle array was used to transport single HeLa cells into nozzle holes. The application of DC-biased AC voltage (100 kHz, 10 Vpp, and VDC: −4 V) provided a sufficient electroosmotic flow outside the nozzle array. This integration method of nozzle and pumps is anticipated to be a standard integration method. The operating conditions of DC-biased AC electrokinetic pumps in a biological buffer was clarified and found useful for cell manipulation.

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Current Trends of Microfluidic Single-Cell Technologies

Cited by 67 publications

References 260 publications

Visualization and Measurements of Blood Cells Flowing in Microfluidic Systems and Blood Rheology: A Personalized Medicine Perspective

Visualization and Measurements of Blood Cells Flowing in Microfluidic Systems and Blood Rheology: A Personalized Medicine Perspective

dMSCC: A microfluidic platform for microbial single-cell cultivation under dynamic environmental medium conditions

Scalable Parallel Manipulation of Single Cells Using Micronozzle Array Integrated with Bidirectional Electrokinetic Pumps

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