This paper aims to address Digital Readiness in Higher Education Institutions from the perspective of data-informed and evidence-based assessment of Digital Readiness. Related research suggests that existing instruments for assessing digitalization aspects are limited to self-assessment, and there is a need for data-informed frameworks that will allow automated assessments based on data that Higher Education Institutions already collect. To bridge this gap, we propose a conceptual, data-informed framework for assessing Digital Readiness in Higher Education Institutions. We followed a participatory approach informed by related theory and practice to design, together with stakeholders from three academic institutions in three European countries, a Digital Readiness assessment framework on seven dimensions accompanied by 72 data-informed indicators. Digitalization of Higher Education is a priority in political agendas, and educational institutions have acknowledged it as an opportunity to promote digitalization of teaching and learning, organizational structures, and technical services, enabling innovation and internationalization. We envision that this work contributes towards supporting educational institutions in self-reflecting upon their digitalization policies and practices.