Curvature Correction Factors in the Measurement of Cell Surface Areas in Plant Tissues1

Thain, J. F.

doi:10.1093/jxb/34.1.87

Cited by 129 publications

(115 citation statements)

References 6 publications

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“…The factor 1.34 accounts for cell surfaces that were not uniformly perpendicular to the plane of the section based on the average width to height ratio for palisade and spongy cells [7,19]. The fraction of mesophyll tissue occupied by the intercellular air spaces (f ias ) was determined as:…”

Section: Methodsmentioning

confidence: 99%

Relationship between CO₂ assimilation and leaf anatomical characteristics of two grapevine cultivars

2003

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-The possible implication of leaf anatomical characteristics on the photosynthetic rate was studied in two grapevine cultivars, Ribier (Vitis vinifera L.) and Isabella (Vitis labrusca), grown under field conditions. Ribier exhibited higher photosynthetic rates than Isabella, although there were no significant differences in the Rubisco activity and the stomatal conductance. The fraction of mesophyll volume represented by the intercellular spaces as well as the surface area of mesophyll cells exposed to intercellular air spaces were significantly lower in Isabella. Both gaseous CO 2 conductance through intercellular airspaces and liquid phase conductance were significantly higher in Ribier than in Isabella, contributing to a higher photosynthetic rate in this cultivar.leaf anatomy / mesophyll conductance / photosynthetic rate / Vitis vinifera / Vitis labrusca Résumé -Relation entre l'assimilation de CO 2 et les caractéristiques anatomiques des feuilles de deux espèces de vigne. L'influence possible des caractéristiques anatomiques des feuilles sur le taux de photosynthèse a été étudiée pour deux espèces de vigne : Vitis Labrusca (Isabella) and Vitis vinifera L. (Ribier), cultivées en culture au champ. Ribier a présenté un taux photosynthétique plus élevé que Isabella, bien qu'il n'y ait pas de différences significatives de l'activité de la Rubisco et de la conductance stomatique. La fraction du volume de mésophylle représentée par l'espace intercellulaire, ainsi que la surface des cellules du mésophylle exposée à l'air dans l'espace intercellulaire, étaient significativement plus faibles chez Isabella. Les conductances gazeuses pour le CO 2 à travers l'espace intercellulaire et en phase liquide étaient significativement plus fortes pour Ribier que pour Isabella, contribuant à des taux photosynthétiques plus importants pour cette espèce.anatomie des feuilles / conductance interne / taux photosynthétique / Vitis vinifera / Vitis labrusca

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Section: Methodsmentioning

confidence: 99%

Relationship between CO₂ assimilation and leaf anatomical characteristics of two grapevine cultivars

2003

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“…The first applications of stereology in plant anatomy were published by Pazourek (1975;, Chabot and Chabot (1977), Parkhurst (1982), Morris andThain (1983), andHajibagheri et al (1984). Later, a number of design-based stereological methods for the estimation of various leaf characteristics were introduced by Kubínová (1987;1989b;1991;1993;.…”

Section: Short History Of Quantification Of Plant Anatomical Structurementioning

confidence: 99%

“…The corresponding areas were measured by a point-counting method, based on counting points of the test grid falling in the tissue under study (Chabot and Chabot, 1977;Pazourek, 1977;Pazourek and Nátr, 1981;Parkhurst, 1982;Hajibagheri et al, 1984;Gowland et al, 1987;Pazourek et al, 1987;Kubínová, 1991;Albrechtová, 1994), by a planimeter (Turrell, 1936), by cutting out the enlarged drawings of the sections and weighing them (El-Sharkawy and Hesketh, 1965;Charles-Edwards et al, 1972;Dengler and MacKay, 1975), by a semiautomatic image analyser (Parker and Ford, 1982) or by a stereological method based on length measurements (linear integration method, Weibel, 1979;Thain, 1983). A number of studies (e.g., Gundersen and Jensen, 1987) showed that, in general, the point-counting method using a regular grid of points (which is positioned uniformly at random on the section) is the most effective one.…”

Section: Plant Tissue Volume Densitymentioning

confidence: 99%

“…This method for the measurement of the surface area of mesophyll cells, also used by Dengler and MacKay (1975) and later by other authors (Parker and Ford, 1982;Barbour and Farquhar, 2004), was criticized and modified by Thain (1983), who proposed several curvature correction factors eliminating the error caused by the curvature of cell surfaces, assuming different model shapes of mesophyll cells. Some of these mesophyll cell model shapes were more realistic than cylinders with hemispheres on each end (assumed by Nobel et al, 1975) or spheres (Bunce et al, 1977).…”

Section: Surface Area Of Mesophyll Cells and Internal Leaf Surface Areamentioning

confidence: 99%

“…Special models of mesophyll cells (cylindrical type, isotropic type, and a rather controversial intermediate type of structure) were assumed. Some of the pitfalls of Parkhurst's approach (disregarding the end surface of cylinder-like cells, subjective estimation of the degree of 'cylindricity' of the tissue) were discussed by Thain (1983). James et al (1999) also used a model-based approach to the measurement of the mesophyll surface area per leaf area from oblique-paradermal sections -the latter exploited Image-Pro Plus software.…”

Section: Surface Area Of Mesophyll Cells and Internal Leaf Surface Areamentioning

confidence: 99%

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Stereology, an Unbiased Methodological Approach to Study Plant Anatomy and Cytology: Past, Present and Future

Kubínová¹,

Radochová²,

Lhotáková³

et al. 2017

Image Anal Stereol

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This review presents an historical overview of stereological methods used for the quantitative evaluation of plant anatomical and cytological structures. It includes the origins of these methods up to the most recent developments such as the application of stereology based on 3D images. We focus especially on leaf, as the vast majority of studies of plant microscopic structure examine this organ. An overview of plant cell ultrastructure measurements as well as plant anatomical characteristics (e.g., plant tissue volume density, internal leaf surface area, number and mean size of mesophyll cells and chloroplast number), which were estimated by stereological methods most frequently, is presented. We emphasize the importance of proper sampling needed for unbiased measurements. Furthermore, we mention other methods used for plant morphometric studies and briefly discuss their relevance, precision, unbiasedness and efficiency in comparison with unbiased stereology. Finally, we discuss reasons for the sparse use of stereology in plant anatomy and consider the future of stereology in plant research.

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Growing at the arid edge: Anatomical variations in leaves are more extensive than in stems of five Mediterranean species across contrasting moisture regimes

Alon,

Ginzburg,

Zemach

et al. 2024

American J of Botany

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PremiseIncreasing aridity in the Mediterranean region affects ecosystems and plant life. Various anatomical changes in plants help them cope with dry conditions. This study focused on anatomical differences in leaves and xylem of five co‐occurring Mediterranean plant species namely Quercus calliprinos, Pistacia palaestina, Pistacia lentiscus, Rhamnus lycioides, and Phillyrea latifolia in wet and dry sites.MethodsStomatal density, stomatal length, leaf mass area, lamina composition, percentage of intercellular air spaces, and mesophyll cell area in leaves of plants in wet and dry sites were analyzed. Xylem anatomy was assessed through vessel length and area in branches.ResultsIn the dry site, three species had increased stomatal density and decreased stomatal length. Four species had increased palisade mesophyll and reduced air space volume. In contrast, phenotypic changes in the xylem were less pronounced; vessel length was unaffected by site conditions, but vessel diameter decreased in two species. Intercellular air spaces proved to be the most dynamic anatomical feature. Quercus calliprinos had the most extensive anatomical changes; Rhamnus lycioides had only minor changes. All these changes were observed in comparison to the species in the wet site.ConclusionsThis study elucidated variations in anatomical responses in leaves among co‐occurring Mediterranean plant species and identified the most dynamic traits. Understanding these adaptations provides valuable insights into the ability of plants to thrive under changing climate conditions.

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Curvature Correction Factors in the Measurement of Cell Surface Areas in Plant Tissues1

Cited by 129 publications

References 6 publications

Relationship between CO₂ assimilation and leaf anatomical characteristics of two grapevine cultivars

Relationship between CO₂ assimilation and leaf anatomical characteristics of two grapevine cultivars

Stereology, an Unbiased Methodological Approach to Study Plant Anatomy and Cytology: Past, Present and Future

Growing at the arid edge: Anatomical variations in leaves are more extensive than in stems of five Mediterranean species across contrasting moisture regimes

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Curvature Correction Factors in the Measurement of Cell Surface Areas in Plant Tissues1

Cited by 129 publications

References 6 publications

Relationship between CO2 assimilation and leaf anatomical characteristics of two grapevine cultivars

Relationship between CO2 assimilation and leaf anatomical characteristics of two grapevine cultivars

Stereology, an Unbiased Methodological Approach to Study Plant Anatomy and Cytology: Past, Present and Future

Growing at the arid edge: Anatomical variations in leaves are more extensive than in stems of five Mediterranean species across contrasting moisture regimes

Contact Info

Product

Resources

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Relationship between CO₂ assimilation and leaf anatomical characteristics of two grapevine cultivars

Relationship between CO₂ assimilation and leaf anatomical characteristics of two grapevine cultivars