Curvature-electric effect in black lipid membranes

Петров, А. Г.; Sokolov, V.S.

doi:10.1007/bf00542559

Cited by 55 publications

(55 citation statements)

References 14 publications

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“…The first indication that flexoelectricity is coupled to transmembrane ion movement was obtained with mechanically-stressed black lipid membranes (BLMs)just prior to their rupture. During this period, metastable, ion-conducting pores were observed and a striking enhancement of the 1st a.c. current harmonic was recorded in the presence of such pores (Petrov and Sokolov 1986). Similar results were later obtained with excised patches of locust muscle membrane , i.e.…”

Section: Introductionsupporting

confidence: 73%

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Flexoelectric effects in model and native membranes containing ion channels

et al. 1993

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An experimental study of flexoelectricity in model membranes containing ion pores and native membranes containing ion channels has been undertaken with the objective of determining the relationship, if any, between flexoelectricity and ion transport. Model membrane patches containing ion pores induced by a blue-green algal toxin, microcystin-LR, and locust muscle membrane patches containing potassium channels were studied using patch-clamp techniques. A correspondence was established between the presence of open channels and pores and the amplitude of the 1st harmonic of the total membrane current when the membranes or patches were subjected to pressure oscillations. The 2nd harmonic of the membrane current provided a measure of the amplitude of a membrane curvature induced by pressure, thus making it possible to determine the membrane flexoelectric coefficient. This study shows that flexoelectricity could be an effective driving force for ion transport through membrane pores and channels, thus further highlighting the possible biological significance of this mechano-electric phenomenon.

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Section: Introductionsupporting

confidence: 73%

“…These currents have the same frequency as the membrane oscillations (Petrov and Sokolov 1986;Derzhanski et al 1981Derzhanski et al , 1989Derzhanski et al , 1990. Flexoelectricity has also been reported in patches excised from natural membranes ).…”

Section: Introductionmentioning

confidence: 63%

Flexoelectric effects in model and native membranes containing ion channels

et al. 1993

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“…It is also linked to changes in capacitance. Electromechanical coupling in membranes was first proposed by Petrov [42,43] and has been discussed by various authors as relevant in hair cells [44,45]. Here, the potential of the lipid membrane is discussed.…”

Section: Electromechanical Couplingmentioning

confidence: 99%

On the Action Potential as a Propagating Density Pulse and the Role of Anesthetics

Heimburg

Jackson

2007

Biophys. Rev. Lett.

118

134

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The Hodgkin-Huxley model of nerve pulse propagation relies on ion currents through specific resistors called ion channels. We discuss a number of classical thermodynamic findings on nerves that are not contained in this classical theory. Particularly striking is the finding of reversible heat changes, thickness and phase changes of the membrane during the action potential. Data on various nerves rather suggest that a reversible density pulse accompanies the action potential of nerves. Here, we attempted to explain these phenomena by propagating solitons that depend on the presence of cooperative phase transitions in the nerve membrane. These transitions are, however, strongly influenced by the presence of anesthetics. Therefore, the thermodynamic theory of nerve pulses suggests a explanation for the famous Meyer-Overton rule that states that the critical anesthetic dose is linearly related to the solubility of the drug in the membranes.

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“…For low oscillation frequencies (< 300 Hz), lipid insertion can significantly change the bilayer polarization. The phenomenon is quite complex, however, it most noticeable at high potentials (> 100 mV) and large membrane deflections [57,54].…”

Section: Bilayer Mechanoelectrical Transductionmentioning

confidence: 99%

“…Pioneering work by Sarles and Leo showed that lipid bilayers, the foundation of the cell membrane, can be used as mechanoelectrical transducers [77,61]. By virtue of the flexoelectric effect, bending of the bilayer membrane due to hair motion induces a change in capacitance that can be measured as ionic current flow across the membrane [55,57]. The fabrication method for these sensors requires that the bilayer be assembled in situ while submerged in an oil solution.…”

Section: Bilayer Based Ahcsmentioning

confidence: 99%

Airflow Sensing With Arrays of Hydrogel Supported Artificial Hair Cells

Sarlo

Leo

2015

Volume 2: Integrated System Design and Implementation; Structural Health Monitoring; Bioinspired Smart Materials and Systems; E

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Arrays of fully hydrogel-supported, artificial hair cell (AHC) sensors based on bilayer membrane mechanotransduction are designed and characterized to determine sensitivity to multiple stimuli. The work draws upon key engineering design principles inspired by the characteristics of biological hair cells, primarily the use of slender hair-like structures as flow measurement elements. Many hair cell microelectromechanical (MEMS) devices to sense fluid flow have already been built based on this principle. However, recent developments in lipid bilayer applications, namely physically encapsulated bilayers and hydrogel interface bilayers, have facilitated the development of AHCs made primarily from biomolecular materials. The most current research in this field of "membrane based AHCs," shows promise, yet still lacks the modularity to create large sensor arrays similar to those in nature. This paper presents a novel bilayer based AHC platform, developed for array implementation by applying some of the core design principles of biological hair cells. These principles are translated into key design, fabrication and material considerations toward improved sensor sensitivity and modularity. Single hair cell responses to base excitation and short air pulses are to investigate the dynamic coupling between hair and bilayer membrane transducer. In addition, a spectral analysis of the AHC system under varying voltages and air flow velocities helps to build simple, predictive models for the sensitivity properties of the AHC. And finally, based on these results, we implement a spatial sensing strategy that involves mapping frequency content to stimulus location by "tuning" linear, three-unit arrays of AHCs.Individual AHC sensors characterization results demonstrate peak current outputs in the nanoamp range and measure flow velocities as high as 72 m/s. Characterization of the AHC response to base excitation and air pulses show that membrane current oscillates with the first three bending modes of the hair. Output magnitudes reflect of vibrations near the base of the hair. A 2 degree-of-freedom Rayleigh-Ritz approximation of the system dynamics yields estimates of 19 N/m and 0.0011 Nm/rad for the equivalent linear and torsional stiffness of the hair's hydrogel base, although double modes suggest non-symmetry in the gel's linear stiffness. The sensor output scales linearly with applied voltage (1.79 pA/V), avoiding a higher-order dependence on electrowetting effects. The free vibration amplitude of the sensor also increases in a linear fashion with applied airflow pressure (3.39 pA/m s −1 ). Array sensing tests show that the bilayers' consistent spectral responses allow for an accurate localization of the airflow source. However, temporal variations in bilayer size affect sensitivity properties and make airflow magnitude estimation difficult. The overall successful implementation of the array sensing method validates the sensory capability of the bilayer based AHC.iii DedicationTo my parents, Guillermo and Maria, and to my...

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Curvature-electric effect in black lipid membranes

Cited by 55 publications

References 14 publications

Flexoelectric effects in model and native membranes containing ion channels

Flexoelectric effects in model and native membranes containing ion channels

On the Action Potential as a Propagating Density Pulse and the Role of Anesthetics

Airflow Sensing With Arrays of Hydrogel Supported Artificial Hair Cells

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