Curvularia lunata as new causal pathogen of tomato early blight disease in Egypt

AbdElfatah, Heba-Alla S.; Sallam, Nashwa M. A.; Mohamed, Mohamed S.; Bagy, Hadeel M. M. Khalil

doi:10.1007/s11033-021-06254-8

“…6B ) may trigger drastic shifts in microbial community structure between disease-promotive and disease-suppressive states ( 3 ). The candidate list of such core species involved an ammonium-oxidizing archaeon ( Nitrosotenuis ) ( 47 ), an antibiotics-producing bacterium ( Streptomyces ) ( 48 ), a prevalent soil fungus ( Mortierella ) ( 49 , 50 ), a potentially mycoparasitic fungus ( Trichoderma ) ( 51 , 52 ), and fungi allied to plant pathogenic clades ( Curvularia and Plectosphaerella [anamorph = Fusarium ]) ( 53 , 54 ) ( Table 5 ). Given that many of the bacterial and fungal taxa listed above are culturable, experimental studies examining their ecological roles are awaited.…”

Section: Discussionmentioning

confidence: 99%

Soil prokaryotic and fungal biome structures associated with crop disease status across the Japan Archipelago

Fujita,

Yoshida,

Suzuki

et al. 2024

mSphere

1

0

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Archaea, bacteria, and fungi in the soil are increasingly recognized as determinants of agricultural productivity and sustainability. A crucial step for exploring soil microbiomes with important ecosystem functions is to perform statistical analyses on the potential relationship between microbiome structure and functions based on comparisons of hundreds or thousands of environmental samples collected across broad geographic ranges. In this study, we integrated agricultural field metadata with microbial community analyses by targeting 2,903 bulk soil samples collected along a latitudinal gradient from cool-temperate to subtropical regions in Japan (26.1–42.8 °N). The data involving 632 archaeal, 26,868 bacterial, and 4,889 fungal operational taxonomic units detected across the fields of 19 crop plant species allowed us to conduct statistical analyses (permutational analyses of variance, generalized linear mixed models, randomization analyses, and network analyses) on the relationship among edaphic factors, microbiome compositions, and crop disease prevalence. We then examined whether the diverse microbes form species sets varying in potential ecological impacts on crop plants. A network analysis suggested that the observed prokaryotes and fungi were classified into several species sets (network modules), which differed substantially in association with crop disease prevalence. Within the network of microbe-to-microbe coexistence, ecologically diverse microbes, such as an ammonium-oxidizing archaeon, an antibiotics-producing bacterium, and a potentially mycoparasitic fungus, were inferred to play key roles in shifts between crop-disease-promotive and crop-disease-suppressive states of soil microbiomes. The bird’s-eye view of soil microbiome structure will provide a basis for designing and managing agroecosystems with high disease-suppressive functions. IMPORTANCE Understanding how microbiome structure and functions are organized in soil ecosystems is one of the major challenges in both basic ecology and applied microbiology. Given the ongoing worldwide degradation of agroecosystems, building frameworks for exploring structural diversity and functional profiles of soil microbiomes is an essential task. Our study provides an overview of cropland microbiome states in light of potential crop-disease-suppressive functions. The large data set allowed us to explore highly functional species sets that may be stably managed in agroecosystems. Furthermore, an analysis of network architecture highlighted species that are potentially used to cause shifts from disease-prevalent states of agroecosystems to disease-suppressive states. By extending the approach of comparative analyses toward broader geographic ranges and diverse agricultural practices, agroecosystem with maximized biological functions will be further explored.

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“…6B) may trigger drastic shifts in microbial community structure between disease-promotive and disease-suppressive states (3). The candidate list of such core species involved an ammonium-oxidizing archaeum ( Nitrosotenuis ) (45), an antibiotics-producing bacterium ( Streptomyces ) (46), a prevalent soil fungus ( Mortierella ) (47, 48), a potentially mycoparasitic fungus ( Trichoderma ) (49, 50), and fungi allied to plant pathogenic clades [ Curvularia and Plectosphaerella (anamorph = Fusarium )] (51, 52) (Table 5). Given that many of the bacterial and fungal taxa listed above are culturable, experimental studies examining their ecological roles are awaited.…”

Section: Discussionmentioning

confidence: 99%

Soil Prokaryotic and Fungal Biome Structures Associated with Crop Disease Status across the Japan Archipelago

Fujita,

Yoshida,

Suzuki

et al. 2023

Preprint

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Archaea, bacteria, and fungi in the soil are increasingly recognized as determinants of agricultural productivity and sustainability. A crucial step for exploring soil microbiomes with high ecosystem functions is to perform statistical analyses on potential relationship between microbiome structure and functions based on comparisons of hundreds or thousands of environmental samples collected across broad geographic ranges. In this study, we integrated agricultural field metadata with microbial community analyses by targeting > 2,000 soil samples collected along a latitudinal gradient from cool-temperate to subtropical regions in Japan (26.1-42.8 N). The data involving 579 archaeal, 26,640 bacterial, and 6,306 fungal amplicon sequencing variants detected across the fields of 19 crop plant species allowed us to conduct statistical analyses on relationship among edaphic factors, microbiome compositions, and crop disease prevalence. We then found that not only compositions of prokaryotic and fungal communities but also balance between prokaryotic and fungal abundance had statistically significant impacts on crop disease status. A network analysis suggested that the prokaryotes and fungi could be classified into several species sets (network modules), which differed substantially in associations with crop disease prevalence. Within the network of microbe-to-microbe coexistence, ammonium-oxidizing archaea and nitrite-oxidizing bacteria were inferred to play some roles in shifts between crop-disease-promotive and crop-disease-suppressive states of soil microbiomes. The bird's-eye view of soil microbiome structure will provide a basis for designing agroecosystems with high nutrient-use efficiency and disease-suppressive functions.

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“…As a plant pathogen, C. lunata causes early blight disease of tomato (Abdelfatah et al, 2021 ), leaf spot of corn (Manzar et al, 2022 ), postharvest fruit rot of banana (Khan and Javaid, 2020 ), rice seedling rot disease (Limtong et al, 2020 ), and foliar necrotic spots of Andropogon grass (Santos et al, 2018 ) and Brassica rapa (Wonglom et al, 2018 ).…”

Section: The High Priority Group From the Who Fpplmentioning

confidence: 99%

“…Identification of plant pathogen C. lunata strains is based mainly on ITS (Abdelfatah et al, 2021 ), Ras-related (Clg2p) gene (Hou et al, 2013 ), TEF1 and glyceraldehyde 3-phosphate dehydrogenase sequences (Garcia-Aroca et al, 2018 ). Culture characteristics, morphology and conidiation pattern are also frequently used for identification in plant pathology and clinics (Dubey et al, 2019 ; Khan and Javaid, 2020 ), and a next generation sequencing based approach has been published recently (Santona et al, 2022 ).…”

Section: The High Priority Group From the Who Fpplmentioning

confidence: 99%

Trans-kingdom fungal pathogens infecting both plants and humans, and the problem of azole fungicide resistance

Pintye,

Bacsó,

Kovács

2024

Front. Microbiol.

3

0

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Azole antifungals are abundantly used in the environment and play an important role in managing fungal diseases in clinics. Due to the widespread use, azole resistance is an emerging global problem for all applications in several fungal species, including trans-kingdom pathogens, capable of infecting plants and humans. Azoles used in agriculture and clinics share the mode of action and facilitating cross-resistance development. The extensive use of azoles in the environment, e.g., for plant protection and wood preservation, contributes to the spread of resistant populations and challenges using these antifungals in medical treatments. The target of azoles is the cytochrome p450 lanosterol 14-α demethylase encoded by the CYP51 (called also as ERG11 in the case of yeasts) gene. Resistance mechanisms involve mainly the mutations in the coding region in the CYP51 gene, resulting in the inadequate binding of azoles to the encoded Cyp51 protein, or mutations in the promoter region causing overexpression of the protein. The World Health Organization (WHO) has issued the first fungal priority pathogens list (FPPL) to raise awareness of the risk of fungal infections and the increasingly rapid spread of antifungal resistance. Here, we review the main issues about the azole antifungal resistance of trans-kingdom pathogenic fungi with the ability to cause serious human infections and included in the WHO FPPL. Methods for the identification of these species and detection of resistance are summarized, highlighting the importance of these issues to apply the proper treatment.

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Curvularia lunata as new causal pathogen of tomato early blight disease in Egypt

Cited by 16 publications

References 26 publications

Soil prokaryotic and fungal biome structures associated with crop disease status across the Japan Archipelago

Soil prokaryotic and fungal biome structures associated with crop disease status across the Japan Archipelago

Soil Prokaryotic and Fungal Biome Structures Associated with Crop Disease Status across the Japan Archipelago

Trans-kingdom fungal pathogens infecting both plants and humans, and the problem of azole fungicide resistance

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