Custom Scanning Hyperspectral Imaging System for Biomedical Applications: Modeling, Benchmarking, and Specifications

Abstract

Prototyping hyperspectral imaging devices in current biomedical optics research requires taking into consideration various issues regarding optics, imaging, and instrumentation. In summary, an ideal imaging system should only be limited by exposure time, but there will be technological limitations (e.g., actuator delay and backlash, network delays, or embedded CPU speed) that should be considered, modeled, and optimized. This can be achieved by constructing a multiparametric model for the imaging system in question. The article describes a rotating-mirror scanning hyperspectral imaging device, its multiparametric model, as well as design and calibration protocols used to achieve its optimal performance. The main objective of the manuscript is to describe the device and review this imaging modality, while showcasing technical caveats, models and benchmarks, in an attempt to simplify and standardize specifications, as well as to incentivize prototyping similar future designs.

Spatial Distortion Assessments of a Low-Cost Laboratory and Field Hyperspectral Imaging System

“…This can be achieved by closing the aperture completely and obtaining several measurements. By obtaining the baseline pixel values for several exposure times, hot pixel background signals can be modelled in each pixel by [35]:hbg,ij=aij×Texp+bij where indices i and j indicate the pixel’s position in the sensor, a ij and b ij are the coefficients that model the linear behavior of the pixel as a function of exposure time T exp , and h bg,ij is the estimated hot pixel value that should be subtracted from each pixel for every new measurement. The data are finally converted to create a pyramid in BSQ formation with an ENVI header.…”

“…This integration eliminates the need for a mobile platform for ground and laboratory data acquisition and allows the imaging system to be used for both airborne, field and laboratory applications [33]. Other types of hyperspectral systems are described in [35] (a rotating-mirror scanning hyperspectral imaging device) and [36] (transition from single pixel spectrometers to an FPA base imaging spectrometer). These approaches are implemented by employing a rotating filter wheel or electrically scanned optical filter in front of a monochrome area camera.…”

Context-free hyperspectral image enhancement for wide-field optical biomarker visualization

“…The imaging system used for hyperspectral image capture is a custom-built hyperspectral scanning system, with an embedded computer for storage and control [25]. A general description is left in Table 1.…”

Polarizer-Free AOTF-Based SWIR Hyperspectral Imaging for Biomedical Applications

“…Hyperspectral imaging (HSI), also known as imaging spectroscopy, is an increasingly powerful non-invasive method in biomedicine [ 1 , 2 , 3 ]. It has been proven effective in multiple applications for accurate localization of pathological tissues and quantitative characterization of their spectral properties.…”