Customizing cellular signal processing by synthetic multi-level regulatory circuits

Gao, Yuanli; Wang, Lei; Wang, Baojun

doi:10.1038/s41467-023-44256-1

Cited by 19 publications

(3 citation statements)

References 144 publications

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“…However, there are relatively few reports on the application of dynamic regulation strategies for the biosynthesis of phenolic acids in microbial cell factories. Recently, Wang et al proposed a concept of “multilevel circuits”, which integrated different tools from DNA, RNA, and protein levels into a regulatory circuit, to avoid the limitations of a single part and achieve more complex functions . Therefore, with the development of synthetic biology, the dynamic regulation strategies will be widely used in the construction of microbial cell factories.…”

Section: Dynamic Regulation Of the Biosynthesis Of Phenolic Acidsmentioning

confidence: 99%

Recent Advances in Microbial Metabolic Engineering for Production of Natural Phenolic Acids

Li,

Lu,

Zou

et al. 2024

J. Agric. Food Chem.

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Phenolic acids are important natural bioactive compounds with varied physiological functions. They are extensively used in food, pharmaceutical, cosmetic, and other chemical industries and have attractive market prospects. Compared to plant extraction and chemical synthesis, microbial fermentation for phenolic acid production from renewable carbon sources has significant advantages. This review focuses on the structural information, physiological functions, current applications, and biosynthesis pathways of phenolic acids, especially advances in the development of metabolically engineered microbes for the production of phenolic acids. This review provides useful insights concerning phenolic acid production through metabolic engineering of microbial cell factories.

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Section: Dynamic Regulation Of the Biosynthesis Of Phenolic Acidsmentioning

confidence: 99%

Recent Advances in Microbial Metabolic Engineering for Production of Natural Phenolic Acids

Li,

Lu,

Zou

et al. 2024

J. Agric. Food Chem.

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show abstract

“…Researchers in and around 2020 developed numerous microfluidic platforms and guides for brain organoid development and maintenance, enabling more accessible paths to the precise delivery of nutrients and pharmaceuticals to organoid complexes-a boon for future biocomputing studies [128][129][130][131]. Further, this time also saw researchers investigate alternative means of information storage and novel material development capable of supporting neural computing platforms using traditional methods as well as DNA programming [132][133][134].…”

Section: On Some Approaches Integrating Organoids In Biocomputingmentioning

confidence: 99%

Organoids, Biocybersecurity, and Cyberbiosecurity—A Light Exploration

Palmer,

Akafia,

Woodson

et al. 2024

Organoids

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Organoids present immense promise for studying organ systems and their functionality. Recently, they have become the subject of exploration outside of purely biomedical uses in multiple directions. We will explore the rapidly evolving landscape of organoid research over the 21st century, discussing significant advancements in organoid research and highlighting breakthroughs, methodologies, and their transformative impact on our understanding of physiology and modeling. In addition, we will explore their potential use for biocomputing and harnessing organoid intelligence, investigate how these miniaturized organ-like structures promise to create novel computational models and processing platforms allowing for innovative approaches in drug discovery, personalized medicine, and disease prediction. Lastly, we will address the ethical dilemmas surrounding organoid research by dissecting the intricate ethical considerations related to the creation, use, and potential implications of these in vitro models. Through this work, the goal of this paper is to provide introductory perspectives and bridges that will connect organoids to cybersecurity applications and the imperative ethical discourse accompanying its advancements with commentary on future uses.

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“…Bacterial biosensors have become indispensable, offering versatility from environmental monitoring to disease diagnosis [16], [19]. A significant trend in this field is the simultaneous detect multiple targets, providing comprehensive outcomes [20], [21], [22]. The output signals capture a spectrum of information, reflecting the continuous and dynamic responses of living cells to their environment.…”

Section: Introductionmentioning

confidence: 99%

Engineering Coupled Consortia-Based Biosensors using Incoherent Feedforward Loops

Daniel,

Huang,

Kravchik

et al. 2024

Preprint

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Synthetic multicellular systems offer the potential for performing complex biological tasks through coordinated cell-to-cell communication. However, the engineering of these systems faces challenges, including managing population ratios, cell growth rates, and asynchronous activity. To minimize the impact of these factors on the multicellular design, we developed coupled bacterial consortia designed for multi-marker detection by employing a stable shared quorum sensing signal, enabling the simultaneous initiation of their activities. The shared signal is driven by an incoherent feedforward loop (IFFL) gene circuit capable of generating a consistently low-level plateau-like signal that persists over 20 cell generations. This stability ensures a consistent synthesis rate of molecules and prevents unintended amplification during signal transduction, allowing for the efficient simultaneous initiation of activities among consortium members. We examined the consortia-based biosensor by monitoring Lactate and Heme in humanized fecal samples for 40 hours. Our findings provide insights into the IFFL system's dynamic response and long-term temporal stability, making them valuable for biotechnological applications. Particularly during the scaling of synthetic multicellular systems, where maintaining consistent cell density ratios among different strains within consortia is critical.

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Customizing cellular signal processing by synthetic multi-level regulatory circuits

Cited by 19 publications

References 144 publications

Recent Advances in Microbial Metabolic Engineering for Production of Natural Phenolic Acids

Recent Advances in Microbial Metabolic Engineering for Production of Natural Phenolic Acids

Organoids, Biocybersecurity, and Cyberbiosecurity—A Light Exploration

Engineering Coupled Consortia-Based Biosensors using Incoherent Feedforward Loops

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