Cut‐off values and significance of Oil Red O‐positive cells in bronchoalveolar lavage fluid

Basset, Céline; Lacoste-Collin, Laetitia; Aziza, Jacqueline; Bes, J. C.; Jozan, S.; Courtade-Saïdi, Monique

doi:10.1111/j.1365-2303.2009.00677.x

Cited by 40 publications

(34 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A similar phenomenon has been reported to take place after intraperitoneal injection of pristane for induction of arthritis . Additionally, several human pathologies are associated with the accumulation of lipid‐laden macrophages in the airways after the macrophages had engulfed fat at distant sites in the body . However, Oil Red O staining of bronchoalveolar lavage cytospin preparations, failed to reveal more than physiological accumulation of lipid‐laden macrophages in the airways of our mice.…”

Section: Discussionsupporting

confidence: 85%

Pulmonary inflammation in mice with collagen‐induced arthritis is conditioned by complete Freund's adjuvant and regulated by endogenous IFN‐γ

et al. 2012

View full text Add to dashboard Cite

Following immunization with collagen II (CII) in complete Freund's adjuvant (CFA), DBA/1 mice develop arthritis of major joints. This collagen-induced arthritis (CIA) is used as a model for rheumatoid arthritis (RA) in man. Inflammatory changes in lung tissue commonly occur in RA. However, evidence for pulmonary inflammation in CIA is scarce and ambiguous. Here, we demonstrate pulmonary inflammation accompanying CIA in wild-type DBA/1 mice. In IFN-γ receptor-deficient (IFN-γR KO) mice, inflammation was more frequent and more severe. Injection of CFA only (without CII) proved to be as efficient in eliciting pulmonary inflammation as immunization with CFA + CII, though being less effective in causing arthritis. Significant correlation in severity between joint and pulmonary involvement could not be demonstrated. Macroscopic, microscopic, and functional characteristics of pulmonary inflammation in the mice resembled those seen in human RA. Increased inflammation in IFN-γR KO mice was accompanied by augmented expression of various cytokines and chemokines, as measured by RT-PCR on affected tissue. Treatment with a TNF-α inhibitor ameliorated lung pathology. We conclude that CIA in DBA/1 mice is accompanied by pulmonary inflammation. Although both disease processes are kept in check by endogenous IFN-γ, lack of strict parallelism indicates that overlap in their pathogeneses is partial. Keywords: Arthritis r IFN-γ r Lung IntroductionFollowing immunization with collagen II (CII) in complete Freund's adjuvant (CFA), DBA/1 mice develop arthritis of major joints [1]. This experimental system, called collagen-induced arthritis (CIA), is used as a study model for rheumatoid arthritis Correspondence: Prof. Patrick Matthys e-mail: patrick.matthys@rega.kuleuven.be (RA) in man [2][3][4]. RA is usually categorized as an autoimmune disease. However, in contrast to what is the case for typical autoimmune diseases, for example type I diabetes, a break in tolerance for any tissue-specific self-antigen has so far not been identified. The disease is systemic at all stages and can affect a multitude of organ systems, including mucosae (Sjögren's syndrome), pericard, pleura, and lungs [5,6]. Pulmonary complications account for 10 to 20% of mortality in RA patients [7,8]. The pleura and the airways, as well as the interstitium and vasculature of the lung can be affected [7,8]. Pleuritis is the most common manifestation of lung disease associated with RA, but this condition often remainswww.eji-journal.eu 3224 Evelien Schurgers et al. Eur. J. Immunol. 2012. 42: 3223-3234 asymptomatic [8,9]. Rheumatoid arthritis-interstitial lung disease (RA-ILD) refers to a diverse range of pulmonary disorders that affect the interstitium of the lung. Three different interstitial pathologies can be distinguished in RA: (i) bronchiolitis, (ii) usual insterstital pneumonia (UIP), and (iii) rheumatoid nodules [6,10,11]. The pathogenesis of extra-articular complications in RA is incompletely understood and evidence for similar multiorgan involvement in C...

show abstract

Section: Discussionsupporting

confidence: 85%

Pulmonary inflammation in mice with collagen‐induced arthritis is conditioned by complete Freund's adjuvant and regulated by endogenous IFN‐γ

et al. 2012

View full text Add to dashboard Cite

show abstract

“…Lipid clearance is ordinarily mediated by ATII cells and, to a lesser extent, AMs (12,13). Lipid-laden AMs, also called "foam cells," are common pathological features in animals models of lung fibrosis (14,15) and in histological specimens from patients with various chronic fibrotic lung diseases (16,17). Although foam cells are thought to be a consequence, rather than cause, of fibrotic lung disease, their very presence signifies a disruption in surfactant lipid homeostasis.…”

Section: Clinical Relevancementioning

confidence: 99%

A Pneumocyte–Macrophage Paracrine Lipid Axis Drives the Lung toward Fibrosis

Romero¹,

Shah²,

Duong³

et al. 2015

Am J Respir Cell Mol Biol

125

107

View full text Add to dashboard Cite

Lipid-laden macrophages, or "foam cells," are observed in the lungs of patients with fibrotic lung disease, but their contribution to disease pathogenesis remains unexplored. Here, we demonstrate that fibrosis induced by bleomycin, silica dust, or thoracic radiation promotes early and sustained accumulation of foam cells in the lung. In the bleomycin model, we show that foam cells arise from neighboring alveolar epithelial type II cells, which respond to injury by dumping lipids into the distal airspaces of the lungs. We demonstrate that oxidized phospholipids accumulate within alveolar macrophages (AMs) after bleomycin injury and that murine and human AMs treated with oxidized phosphatidylcholine (oxPc) become polarized along an M2 phenotype and display enhanced production of transforming growth factor-b1. The direct instillation of oxPc into the mouse lung induces foam cell formation and triggers a severe fibrotic reaction. Further, we show that reducing pulmonary lipid clearance by targeted deletion of the lipid efflux transporter ATPbinding cassette subfamily G member 1 increases foam cell formation and worsens lung fibrosis after bleomycin. Conversely, we found that treatment with granulocyte-macrophage colony-stimulating factor attenuates fibrotic responses, at least in part through its ability to decrease AM lipid accumulation. In summary, this work describes a novel mechanism leading to foam cell formation in the mouse lung and suggests that strategies aimed at blocking foam cell formation might be effective for treating fibrotic lung disorders.

show abstract

“…The overall data are shown in Table 2. Compared with normal values, 10–12 HIV‐infected patients BALF samples were characterized by elevated percentages of neutrophils (10% [5–25]) and ORO‐PM (40% [6–80]), as expected according to our latest study 10 . We choose to compare BALF percentages rather than absolute numbers because it has been reported that despite variability in the cell numbers between subjects, the differential cell counts represent the most consistent parameter on repeated BALF analyses 12 .…”

Section: Resultsmentioning

confidence: 91%

“…BALF was performed as previously described 10 . Aliquots were transported to the cytology laboratory at 4 °C.…”

Section: Methodsmentioning

confidence: 99%

“…The presence of an alveolitis or an increase in the numbers of alveolar macrophages and of CD3 + T cells have been associated with a decreased mortality while low numbers of lung CD4 + T cells have been associated with increased mortality 9 . We recently found an excess of lipid‐laden macrophages (assessed by Oil Red O staining) in BALF of HIV‐infected patients 10 when compared with other pathological groups of HIV‐negative patients. Such an increase could be linked to the virus or to the antiretroviral treatment, especially protease inhibitors, as they are responsible for lipid disorders.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Investigation of the significance of Oil Red O‐positive macrophage excess in bronchoalveolar lavage fluid during HIV infection

et al. 2011

Self Cite

View full text Add to dashboard Cite

show abstract

Cut‐off values and significance of Oil Red O‐positive cells in bronchoalveolar lavage fluid

Abstract: Significantly increased numbers of ORO-PM were associated with various lung pathologies. However, the higher numbers observed in HIV patients require further investigations.

Cited by 40 publications

References 19 publications

Pulmonary inflammation in mice with collagen‐induced arthritis is conditioned by complete Freund's adjuvant and regulated by endogenous IFN‐γ

Pulmonary inflammation in mice with collagen‐induced arthritis is conditioned by complete Freund's adjuvant and regulated by endogenous IFN‐γ

A Pneumocyte–Macrophage Paracrine Lipid Axis Drives the Lung toward Fibrosis

Investigation of the significance of Oil Red O‐positive macrophage excess in bronchoalveolar lavage fluid during HIV infection

Contact Info

Product

Resources

About