Cutaneous Clear Cell Adnexal Carcinoma in a Dog: Special Reference to Cytokeratin Expression

Yasuno, Kyohei; Nishiyama, Shoko; Suetsugu, Fumio; Ogihara, Kikumi; Madarame, Hiroo; Shirota, Kinji

doi:10.1292/jvms.001513

Cited by 11 publications

(45 citation statements)

References 12 publications

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“…d) Trypsin=0.1% trypsin (Dako A/S); MW=microwave, citrate buffer (PH 6.0). Proteinase K=0.4 mg/m l proteinase K (Wako Pure Chemical Industries Ltd. Osaka; Japan), AC=autocleve, citrate buffer (PH 6.0); Pepsin=0.4% pepsin (Sigma-Aldrich Co., St. Louis, MO, U.S.A.). [11]. Peroxidase-conjugated anti-mouse (Histofine Simple Stain MAX-PO (M); Nichirei Corp., Tokyo, Japan) or anti-rabbit (Histofine Simple Stain MAX-PO (R); Nichirei) immunoglobulin (Ig) G was used as secondary antibodies.…”

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confidence: 99%

Trichoblastoma with Abundant Plump Stromal Cells in a Dog

Mineshige

Yasuno

Sugahara

et al. 2014

The Journal of Veterinary Medical Science

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Histopathological and immunohistochemical examinations were made on a cutaneous tumor on the head of an 11-year-old female mixed-breed dog. The tumor was well demarcated and comprised multilobular structures of neoplastic epithelial cells with abundant plump peritumoral stromal cells. The neoplastic cells formed irregular cell cords or trabeculae and were arranged in characteristic palisades at the periphery. Immunohistochemically, neoplastic cells were positive for p63 and the several cytokeratins examined. In contrast, the plump peritumoral stromal cells were positive for vimentin and unevenly for nestin, a neuroepithelial stem cell protein. The stromal cells prominently proliferated in proximity to epithelial neoplastic cells, suggesting a close interaction between these two cell types.

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confidence: 99%

Trichoblastoma with Abundant Plump Stromal Cells in a Dog

Mineshige

Yasuno

Sugahara

et al. 2014

The Journal of Veterinary Medical Science

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“…Trichoblastomas are thought to originate from hair germ cells, and so tumour cells are positive for CK8, but negative for CK18, which is only expressed in sweat glands. Conversely, clear cell adnexal carcinomas show positive staining for both CK8 and CK18 (Kato et al 2007;Yasuno et al 2009;Sakuma et al 2010). Thus, the weak expression of CK18 in the present case excluded the possibility of clear cell adnexal carcinoma.…”

Section: Discussionmentioning

confidence: 43%

Immunohistochemical characterisation of a canine case of granular cell type trichoblastoma: a case report

Yoon¹,

Park²

2014

Vet. Med. - Czech

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Herein we describe a case of granular cell type trichoblastoma in a dog. A nine-year-old, intact male Scottish terrier presented with a solitary, exophytic and non-ulcerated nodule on the shoulder. Histopathologically, the nodule showed a ribbon-like structure, which was composed of branching cords of epithelial cells surrounded by fibrous stroma. In addition, islands of large clear cells were also noted. The clear cells showed eccentric nuclei and abundant cytoplasmic vacuoles, which were strongly positive for periodic acid-Schiff staining. Cytoplasmic immunostaining for cytokeratin (CK) 14 and nuclear immunostaining for p63 were observed in the neoplastic cells. In contrast, CK18 staining was scant. Furthermore, the hair bulge stem cell marker CK15 showed strong cytoplasmic staining. Based on these histopathological and immunohistochemical findings, the tumour was diagnosed as a granular cell type trichoblastoma, which is rarely reported in dogs. Keywords: dog; trichoblastoma; immunohistochemistry; cytokeratinList of abbreviations BCC = Basal cell carcinoma, CK = cytokeratin; H&E = haematoxylin and eosin; PAS = periodic acid-Schiff

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“…12 Cytokeratin (KL1) is expressed in differentiated keratinocytes, 16,17 and the squamous cells in the present case were also positive for CK (KL1). The findings strongly support the conclusion that the present case developed into mature sebocytes and squamous cells.…”

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“…Sebocyte-like cells and basaloid reserve-like cells expressed CK14 and p63, which are also expressed in normal mature sebaceous gland cells 17 and sebaceous basaloid reserve cells.…”

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Mammary adenoma with sebaceous differentiation in a dog

Yasuno

Takagi²,

Kobayashi³

et al. 2011

J VET Diagn Invest

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Abstract. The current report describes a complex canine mammary adenoma with a rare histological feature characterized by sebaceous differentiation of tumor cells. A 13-year-old, mixed-breed, intact female dog had mammary tumors on the right mammary chain. Histologically, one of the masses was composed of bilayered ductal structures with luminal epithelial cells together with basaloid or myoepithelial cell components. Within the tumor, there were a number of lobules and nests of large foamy cells associated with basaloid reserve-like cells similar to sebaceous gland. Squamous metaplasia was also seen within the tumor. Immunohistochemical staining indicated that the tumor cells with sebaceous differentiation were positive for cytokeratin (CK)14 and that the associated basaloid reserve-like cells were positive for p63. In contrast, other luminal epithelial tumor cells were positive for CK18 and CK19, but not for CK14 and p63. The myoepithelial cells were positive for α-smooth muscle actin and p63. The expression of p63 in both sebaceous basaloid reserve-like cells and myoepithelial cells, and their structural continuity within the tumor tissue, suggested a common origin of these 2 components.

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Cutaneous Clear Cell Adnexal Carcinoma in a Dog: Special Reference to Cytokeratin Expression

Cited by 11 publications

References 12 publications

Trichoblastoma with Abundant Plump Stromal Cells in a Dog

Trichoblastoma with Abundant Plump Stromal Cells in a Dog

Immunohistochemical characterisation of a canine case of granular cell type trichoblastoma: a case report

Mammary adenoma with sebaceous differentiation in a dog

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