Cutaneous human papillomavirus 88: Remarkable differences in viral load

Kullander, Johanna; Handisurya, Alessandra; Forslund, Ola; Geusau, Alexandra; Kirnbauer, Reinhard; Dillner, Joakim

doi:10.1002/ijc.23115

Cited by 15 publications

(16 citation statements)

References 23 publications

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“…We found that SK lesions harboured higher amounts of virus than AK; however, due to the small number of observations, this finding requires further investigation. Also, when only minute amounts of virus are present, the possibility that results may reflect contamination rather than actual infection needs to be considered, in particular with regard to the remarkable viral loads of cutaneous HPV-88 described recently (Kullander et al, 2008). We found that many HPV-positive lesions contained higher amounts of virus on top of the lesion than in the lesion itself, in line with previous reports (Forslund et al, 2004).…”

Section: Discussionsupporting

confidence: 91%

Four novel human betapapillomaviruses of species 2 preferentially found in actinic keratosis

Vasiljević

Hazard

Dillner

et al. 2008

Journal of General Virology

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Recent studies have suggested an association between human papillomaviruses (HPVs), particularly species 2 members of the genus Betapapillomavirus, and squamous cell carcinoma (SCC) of the skin. As most of these viruses are uncharacterized, molecular characterization and epidemiology are needed to advance our understanding of their significance in carcinogenesis. This study determined the complete genomes of four betapapillomaviruses of species 2 from skin lesions designated , an isolate of an unpublished HPV type, and analysed their prevalence and viral loads in biopsies from SCC, actinic keratosis (AK), basal cell carcinoma, seborrhoeic keratosis and the healthy skin of 263 immunocompetent patients by HPV type-specific real-time PCR assays. Seventeen patients (6.5 %) harboured at least one of the four HPV types in their lesion, whereas seven patients (2.7 %) harboured one or more of the HPV types in healthy skin. Overall, the four viruses were more common in AK than in healthy skin (odds ratio 5.0, 95 % confidence interval 1.4-17.5), but the prevalence and viral loads were low. This characterization of expands the heterogeneity of members of species 2 of the genus Betapapillomavirus. However, as these types were found in only a few samples and in low amounts, a possible role in carcinogenesis remains elusive.

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Section: Discussionsupporting

confidence: 91%

Four novel human betapapillomaviruses of species 2 preferentially found in actinic keratosis

Vasiljević

Hazard

Dillner

et al. 2008

Journal of General Virology

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“…Skin samples from SCC, AK, seborrhoeic keratosis (SK), and BCC commonly contain multiple HPV types, but typically at very low viral loads [4], [5], [7], [19], [30], [31]. Conventional methods for virus detection suffer from low throughput and are furthermore biased towards identifying sequences homologous to the PCR primers used.…”

Section: Introductionmentioning

confidence: 99%

“…Conventional methods for virus detection suffer from low throughput and are furthermore biased towards identifying sequences homologous to the PCR primers used. There are several examples of NMSC lesions that contain very high amounts of previously uncharacterized HPV types that may escape detection by PCR systems based on known HPV sequences [7], [30]. This has made it difficult for epidemiologic studies to perform an unbiased assessment of which HPV types are present in healthy skin as compared to benign tumors or NMSCs.…”

Section: Introductionmentioning

confidence: 99%

Unbiased Approach for Virus Detection in Skin Lesions

et al. 2013

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To assess presence of virus DNA in skin lesions, swab samples from 82 squamous cell carcinomas of the skin (SCCs), 60 actinic keratoses (AKs), paraffin-embedded biopsies from 28 SCCs and 72 kerathoacanthomas (KAs) and fresh-frozen biopsies from 92 KAs, 85 SCCs and 92 AKs were analyzed by high throughput sequencing (HTS) using 454 or Ion Torrent technology. We found total of 4,284 viral reads, out of which 4,168 were Human Papillomavirus (HPV)-related, belonging to 15 known (HPV8, HPV12, HPV20, HPV36, HPV38, HPV45, HPV57, HPV59, HPV104, HPV105, HPV107, HPV109, HPV124, HPV138, HPV147), four previously described putative (HPV 915 F 06 007 FD1, FA73, FA101, SE42) and two putatively new HPV types (SE46, SE47). SE42 was cloned, sequenced, designated as HPV155 and found to have 76% similarity to the most closely related known HPV type. In conclusion, an unbiased approach for viral DNA detection in skin tumors has found that, although some new putative HPVs were found, known HPV types constituted most of the viral DNA.

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“…To preferentially amplify HPV, MDA with random and HPV-generic primers was done on 83 SCC biopsies (26). After digestion with 5 units HincII (New England Biolabs), the samples were run on a 1% agarose gel.…”

Section: Introductionmentioning

confidence: 99%

Staphylococcus aureusand Squamous Cell Carcinoma of the Skin

Kullander

Forslund

Dillner

2009

Cancer Epidemiology, Biomarkers &Amp; Prevention

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Squamous cell carcinoma (SCC) of the skin is a tumor with greatly increased incidence among immunosuppressed patients; therefore, an infectious cause of SCC has long been sought. We performed a hospital-based case-control study of Staphylococcus aureus and biopsies of SCC (n = 82), basal cell carcinoma (n = 142), actinic keratosis (n = 57), and seborrhoeic keratosis (n = 72) in comparison with biopsies from healthy skin of these 353 immunocompetent patients. In a S. aureusspecific PCR, targeting the nuc gene, presence of S. aureus DNA was strongly associated with SCC (29.3% positive specimens; adjusted odds ratio, 6.23; 95% confidence interval, 3.10-12.53) compared with healthy skin (5.7% positive specimens). There was also a tendency for association of S. aureus with actinic keratosis, but no association was found for basal cell carcinoma or seborrhoeic keratosis. Analysis using cotton swab samples taken on top of the lesions and from healthy skin gave similar results (adjusted odds ratio for SCC compared with healthy skin, 2.67; 95% confidence interval, 1.47-4.83). In conclusion, there is a strong association between SCC and presence of S. aureus. The study design used cannot determine whether the association implies that presence of S. aureus might influence carcinogenesis or whether it may imply that SCC has an increased susceptibility to S. aureus colonization. (Cancer Epidemiol Biomarkers Prev 2009;18(2):472 -8)

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Cutaneous human papillomavirus 88: Remarkable differences in viral load

Cited by 15 publications

References 23 publications

Four novel human betapapillomaviruses of species 2 preferentially found in actinic keratosis

Four novel human betapapillomaviruses of species 2 preferentially found in actinic keratosis

Unbiased Approach for Virus Detection in Skin Lesions

Staphylococcus aureusand Squamous Cell Carcinoma of the Skin

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