Cutaneous infiltration of plasmacytoid dendritic cells and T regulatory cells in skin lesions of polymorphic light eruption

Arisi, Mariachiara; Lonardi, Silvia; Lorenzi, Luisa; Ungari, Marco; Serana, Federico; Fusano, Marta; Moggio, Erica; Calzavara-Pinton, P; Venturini, Marina

doi:10.1111/jdv.14866

Cited by 16 publications

(12 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Molina-Ruiz et al [43], Wackernagel et al [44] and Lei et al [45] found no CD123+ PDCs in skin biopsies of PLE patients. In contrast to these studies, Rossi et al claimed PDC S may play a significant role in the development of PLE, and PLE skin biopsies showed that the dermal distribution of PDCs promotes possible association with CLE [46]. PLE and LE are photosensitive diseases, and UV-B exposure exacerbates the clinical findings of both diseases [1].…”

Section: Discussionmentioning

confidence: 97%

CD123 immunoexpression in cutaneous lupus erythematosus, polymorphous light eruption, pityriasis rosea, and mycosis fungoides

Karagün¹,

Gamsızkan²,

Buyucek³

et al. 2021

pdia

View full text Add to dashboard Cite

Introduction: CD123-positive plasmacytoid dendrocytes are prominent in the infiltrate of cutaneous lupus erythematous. Aim: To determine the significance of the CD123 immunostain, which labels plasmacytoid dendritic cells (PDC), in cutaneous lupus erythematous (CLE), polymorphous light eruption (PLE), pityriasis rosea (PR) and mycosis fungoides (MF). Material and methods: A total of 76 cases, including MF (n = 27), CLE (n = 19), PR (n = 19), and PLE (n = 11), were included in the study after reviewing their diagnostic clinical features and pathologic findings. The primary antibody against CD123 was performed in all cases. Results: CD123+ immunostaining in PDCs was positive in all cases. The highest mean percentage was noted in CLE (15.2%), followed by PLE (15%), PR (8.8%), and MF (2%). Besides, the clustering of CD123-positive cells was significant in CLE and PLE compared to MF and PR. Conclusions: PDC may have an important role in the aetiology of PLE and CLE cases. CD123 is a useful marker for differentiating CLE and PLE from MF and PR.

show abstract

Section: Discussionmentioning

confidence: 97%

CD123 immunoexpression in cutaneous lupus erythematosus, polymorphous light eruption, pityriasis rosea, and mycosis fungoides

Karagün¹,

Gamsızkan²,

Buyucek³

et al. 2021

pdia

View full text Add to dashboard Cite

show abstract

“…Scarring alopecias lupus erythematosus _associated alopecia: all ≥10% plasmacytoid dendritic cell content, plasmacytoid dendritic cell clusters in all cases and superficial and deeper dermal and perieccrine distribution (100% of cases) with involvement of the dermo-epidermal junction, intense and diffuse myxovirus resistance protein A staining lichen planopilaris and frontal fibrosing alopecia: majority <10% plasmacytoid dendritic cell content, rare plasmacytoid dendritic cell clusters, upper dermal distribution surrounding hair infundibula with rare dermo-epidermal junction involvement, mostly patchy myxovirus resistance protein A staining central centrifugal cicatricial alopecia: majority <10% plasmacytoid dendritic cell content, rare plasmacytoid dendritic cell clusters alopecia areata versus trichotillomania plasmacytoid dendritic cells more abundant and myxovirus resistance protein A expression significantly higher in alopecia areatathan in trichotillomania alopecia areata: plasmacytoid dendritic cells in peribulbar location, intense and diffuse myxovirus resistance protein A staining of hair bulbs and peribulbar infiltrate Trichotillomania: superficial perivascular and/or peri-follicular plasmacytoid dendritic cells , patchy myxovirus resistance protein A staining mainly in the epidermis, upper follicular epithelium and/or inflammatory infiltrate plasmacytoid dendritic cells more abundant, formed clusters and infiltrated neoplastic epithelium significantly more in keratoacanthoma than in squamous cell carcinoma myxovirus resistance protein A expression significantly greater in keratoacanthoma than in squamous cell carcinoma showed either negative findings or controversial results. No significant differences were found when comparing plasmacytoid dendritic cells in lichen planus versus lichen striatus, 40 lichen planus versus pityriasis lichenoides, 41 graft-versus-host disease versus drug eruptions, 42 lupus erythematosus versus polymorphous light eruption, 43,44 and lupus erythematosus versus perniosis. 45…”

Section: Clinical Entities Plasmacytoid Dendritic Cell Parametersmentioning

confidence: 89%

Diagnostic utility of plasmacytoid dendritic cells in dermatopathology

Bardawil¹,

Khalil²,

Kurban³

et al. 2021

IJDVL

View full text Add to dashboard Cite

Differentiating cutaneous diseases that mimic each other clinically and histopathologically can at times be a challenging task for the dermatopathologist. At the same time, differentiation of entities with overlapping features may be crucial for patient management. Although not seen in normal skin, plasmacytoid dendritic cells usually infiltrate the skin in several infectious, inflammatory/autoimmune and neoplastic entities. Plasmacytoid dendritic cells can be identified in tissue using specific markers such as CD123 and/or blood-derived dendritic cell antigen-2. Plasmacytoid dendritic cells are the most potent producers of type I interferons and their activity may therefore be assessed indirectly in tissue using human myxovirus resistance protein A, a surrogate marker for type I interferon production. In recent years, accumulating evidence has established the utility of evaluating for specific plasmacytoid dendritic cell-related parameters (plasmacytoid dendritic cell content, distribution and clustering and/ or human myxovirus resistance protein A expression) as a diagnostic tool in differentiating cutaneous diseases with overlapping features such as the alopecias, lupus and its mimics, and neoplastic entities. In this review, we provide an update on the current evidence on this topic and on the contexts where this can be a useful adjunct to reach the histopathological diagnosis.

show abstract

“…Intriguingly, PLE patients exhibit low regulatory T (Treg) cell numbers early on in the season, and these numbers increase as the season advances (49,50). Moreover, photohardening with narrowband-UVB increased the functional capacity of Tregs in PLE (49,51,52). It is interesting to note that Tregs can affect other T cells, and especially CD8 + T cells, during the effector stage and reduce their functional abilities (53,54).…”

Section: Discussionmentioning

confidence: 99%

Accumulation of Cytotoxic Skin Resident Memory T Cells and Increased Expression of IL-15 in Lesional Skin of Polymorphic Light Eruption

et al. 2022

View full text Add to dashboard Cite

Patients with polymorphic light eruption (PLE) develop lesions upon the first exposure to sun in spring/summer, but lesions usually subside during season due to the natural (or medical) photohardening. However, these lesions tend to reappear the following year and continue to do so in most patients, suggesting the presence of a disease memory. To study the potential role of skin resident memory T cells (Trm), we investigated the functional phenotype of Trm and the expression of IL-15 in PLE. IL-15 is known to drive Trm proliferation and survival. Multiplex immunofluorescence was used to quantify the expression of CD3, CD4, CD8, CD69, CD103, CD49a, CD11b, CD11c, CD68, granzyme B (GzmB), interferon-gamma (IFN-γ), and IL-15 in formalin-fixed, paraffin-embedded lesional skin samples from PLE patients and healthy skin from control subjects. Unlike the constitutive T cell population in healthy skin, a massive infiltration of T cells in the dermis and epidermis was observed in PLE, and the majority of these belonged to CD8+ T cells which express Trm markers (CD69, CD103, CD49a) and produced cytotoxic effector molecules GzmB and IFN-γ. Higher numbers of CD3+ T cells and CD11b+CD68+ macrophages produced IL-15 in the dermis as compared to healthy skin. The dominant accumulation of cytotoxic Trm cells and increased expression of IL-15 in lesional skin of PLE patients strongly indicates the potential role of skin Trm cells in the disease manifestation and recurrence.

show abstract

Cutaneous infiltration of plasmacytoid dendritic cells and T regulatory cells in skin lesions of polymorphic light eruption

Abstract: D-PDCS and Tregs may play a significant role in the development of PLE, and dermal distribution of PDCs in PLE skin biopsies seems to confirm a possible overlap with cutaneous lupus erythematosus (CLE).

Cited by 16 publications

References 37 publications

CD123 immunoexpression in cutaneous lupus erythematosus, polymorphous light eruption, pityriasis rosea, and mycosis fungoides

CD123 immunoexpression in cutaneous lupus erythematosus, polymorphous light eruption, pityriasis rosea, and mycosis fungoides

Diagnostic utility of plasmacytoid dendritic cells in dermatopathology

Accumulation of Cytotoxic Skin Resident Memory T Cells and Increased Expression of IL-15 in Lesional Skin of Polymorphic Light Eruption

Contact Info

Product

Resources

About