Cutaneous Malassezia flora in atopic dermatitis differs between adults and children

Takahata, Yumiko; Sugita, Takashi; Katô, Hiroshi; Nishikawa, Akemi; Hiruma, Masataro; Murakami, Masahiko

doi:10.1111/j.1365-2133.2007.08193.x

Cited by 49 publications

(28 citation statements)

References 33 publications

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“…The assessment of additional types of clinical samples is indicated to determine the broad applicability of the approach. Recently, non-culture based techniques which avoided the culture process and directly extracted fungal DNA and differentiated Malassezia species was introduced 22,23 . Further studies are required to evaluate this new technique and compare the differences in the rate of successful identification on the scalp in seborrheic dermatitis patients.…”

Section: Discussionmentioning

confidence: 99%

Distribution ofMalasseziaSpecies on the Scalp in Korean Seborrheic Dermatitis Patients

et al. 2011

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Background:Malassezia species play an important role in the pathogenesis of seborrheic dermatitis. In particular, M. restricta and M. globosa are considered to be the predominant organisms in seborrheic dermatitis of Western countries. However, species distribution of Malassezia in seborrheic dermatitis has not been clearly determined yet in Asia. Objective: To identify the distribution of Malassezia species on the scalp of seborrheic dermatitis patients in Korea using 26S rDNA PCR-RFLP analysis. Methods: A total of 40 seborrheic dermatitis patients and 100 normal healthy volunteers were included in this study. For the identification of Malassezia species, the scalp scales of the subjects were analyzed by 26S rDNA PCR-RFLP analysis. Results: The most commonly identified Malassezia species were M. restricta in the seborrheic dermatitis patients, and M. globosa in the normal controls. In the seborrheic dermatitis group, M. restricta was identified in 47.5%, M. globosa in 27.5%, M. furfur in 7.5%, and M. sympodialis in 2.5% of patients. In the healthy control group, M. globosa was identified in 32.0%, M. restricta in 25.0%, M. furfur in 8.0%, M. obtusa in 6.0%, M. slooffiae in 6.0%, and M. sympodialis in 4.0% of subjects. Conclusion: M. restricta is considered to be the most important Malassezia species in Korean seborrheic dermatitis patients. (Ann Dermatol 23(2) 156∼161, 2011)

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Section: Discussionmentioning

confidence: 99%

Distribution ofMalasseziaSpecies on the Scalp in Korean Seborrheic Dermatitis Patients

et al. 2011

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“…Atopic dermatitis is a chronic, fluctuating, inflammatory skin disease that rarely presents in adulthood; it commonly occurs within the first 2 years after the birth. The worldwide prevalence of AD is 10∼ 20% in children and 1∼3% in adults 23 . So in this study, we enrolled AD patients from the 0∼30 age group, and not from the AD patients of all ages.…”

Section: Discussionmentioning

confidence: 99%

Molecular Analysis of Malassezia Microflora on the Skin of the Patients with Atopic Dermatitis

Yim

Kim

et al. 2010

Ann Dermatol

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Background:The yeasts of the genus Malassezia are members of the normal flora on human skin and they are found in 75∼80% of healthy adults. Since its association with various skin disorders have been known, there have been a growing number of reports that have implicated Malassezia yeast in atopic dermatitis (AD). Objective: The aim of the present study is to isolate the various Malassezia species from AD patients by using 26S rDNA (ribosomal Deoxyribonucleic acid) PCR-RFLP and to investigate the relationship between a positive Malassezia culture and the severity of AD. Methods: Cultures for Malassezia yeasts were taken from the scalp, cheek, chest, arm and thigh of 60 patients with atopic dermatitis. We used a rapid and accurate molecular biological method 26S rDNA PCR-RFLP, and this method can overcome the limits of the morphological and biochemical methods. Results: Positive Malassezia growth was noted on 51.7% of the patients with atopic dermatitis by 26S rDNA PCR-RFLP analysis. The overall dominant species was M. sympodialis (16.3%). M. restricta was the most common species on the scalp (30.0%) and cheek (16.7%). M. sympodialis (28.3%) was the most common species on the chest. The positive culture rate was the highest for the 11 ∼20 age group (59.0%) and the scalp showed the highest rate at 66.7%. There was no significant relationship between the Malassezia species and SCORing for Atopic Dermatitis (SCORAD). Conclusion: The fact that the cultured species was different for the atopic dermatitis lesion skin from that of the normal skin may be due to the disrupted skin barrier function and sensitization of the organism induced by scratching in the AD lesion-skin. But there was no relationship between the Malassezia type and the severity score. The severity score is thought to depend not on the type, but also on the quantity of the yeast.

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“…Japonya'da yapılan bu çalışmalarda AD olgularında Malassezia yükü-nün sağlıklılardan çok fazla olduğu gösterilmiştir. [10][11][12][13] Fakat bu bulgular bizim çalışmamıza dahil ettiğimiz Türk hastalar ile doğrulanmamıştır. Bu birbirinden farklı iki sonuç derideki Malassezia kolonizasyonunun coğra-fik ve etnik faktörlerden etkilenebileceğini göstermektedir.…”

Section: Discussionunclassified

Atopik Dermatitli Hastalarda Malassezia Yükünün Moleküler Yöntemler ile Gösterilmesi

Önder¹,

Kalkancı²,

Kevlekçi³

et al. 2011

Turkderm

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Cutaneous Malassezia flora in atopic dermatitis differs between adults and children

Abstract: The cutaneous Malassezia flora differs significantly between the two age groups.

Cited by 49 publications

References 33 publications

Distribution ofMalasseziaSpecies on the Scalp in Korean Seborrheic Dermatitis Patients

Distribution ofMalasseziaSpecies on the Scalp in Korean Seborrheic Dermatitis Patients

Molecular Analysis of Malassezia Microflora on the Skin of the Patients with Atopic Dermatitis

Atopik Dermatitli Hastalarda Malassezia Yükünün Moleküler Yöntemler ile Gösterilmesi

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