Cutaneous photoprotection from ultraviolet injury by green tea polyphenols

Elmets, Craig A.; Singh, Divya; Tubesing, Karen A.; Matsui, Mary S.; Katiyar, Säntosh K.; Mukhtar, Hasan

doi:10.1067/mjd.2001.112919

Cited by 330 publications

(226 citation statements)

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“…Studies have shown that oral feeding as well as topical application of polyphenolic fraction isolated from GTP affords protection against the carcinogenic effects of UVB radiation (Wang et al, 1991;Katiyar et al, 1995). We have also shown that topical application of GTP to humans reduced the number of sunburn cells and protected epidermal Langerhans cells from UV damage (Elmets et al, 2001). We first assessed whether topical application of GTP, after repeated exposure of the mice to UVB, inhibits skin edema and infiltration of leukocytes.…”

Section: Discussionmentioning

confidence: 99%

“…Based on several in vitro and in vivo studies, polyphenols present in green tea, most notably its major constituent epigallocatechin-3-gallate (EGCG), have been shown to exert antioxidant activity and photochemopreventive effects (Elmets et al, 2001;Katiyar and Mukhtar, 2001;Katiyar et al, 2001a, b;Kim et al, 2001;Nomura et al, 2001;Afaq et al, 2003a). Previous cell culture studies from our laboratory have shown that EGCG inhibits UVBmediated phosphorylation of MAPKs (Katiyar et al, 2001a) and activation of NF-kB pathways in normal human epidermal keratinocytes (NHEK) (Afaq et al, 2003a).…”

Section: Introductionmentioning

confidence: 99%

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Suppression of UVB-induced phosphorylation of mitogen-activated protein kinases and nuclear factor kappa B by green tea polyphenol in SKH-1 hairless mice

2003

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Studies from our laboratory have shown that epigallocatechin-3-gallate, the major polyphenol present in green tea, inhibits ultraviolet (UV)B-exposure-mediated phosphorylation of mitogen-activated protein kinases (MAPKs) (Toxicol. Appl. Pharmacol. 176: 110-117, 2001) and activation of nuclear factor kappa B (NF-jB) (Oncogene 22: 1035(Oncogene 22: -1044(Oncogene 22: , 2003 pathways in normal human epidermal keratinocytes. This study was designed to investigate the relevance of these findings to the in vivo situations in SKH-1 hairless mouse model, which is regarded to have relevance to human situations. SKH-1 hairless mice were topically treated with GTP (5 mg/ 0.2 ml acetone/mouse) and were exposed to UVB 30 min later (180 mJ/cm 2 ). These treatments were repeated every alternate day for 2 weeks, for a total of seven treatments. The animals were killed 24 h after the last UVB exposure. Topical application of GTP resulted in significant decrease in UVB-induced bifold-skin thickness, skin edema and infiltration of leukocytes. Employing Western blot analysis and immunohistochemical studies, we found that GTP resulted in inhibition of UVB-induced: (i) phosphorylation of extracellular-signal-regulated kinases (ERK1/2), (ii) c-Jun N-terminal kinases, and (iii) p38 protein expression. Since NF-jB plays a major role in inflammation and cell proliferation, we assessed the effect of GTP on UVB-mediated modulations in the NF-jB pathway. Our data demonstrated that GTP inhibited UVB-induced: (i) activation of NF-jB, (ii) activation of IKKa, and (iii) phosphorylation and degradation of IjBa. Our data suggest that GTP protects against the adverse effects of UV radiation via modulations in MAPK and NF-jB signaling pathways, and provides molecular basis for the photochemopreventive effect of GTP in an in vivo animal model system.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Suppression of UVB-induced phosphorylation of mitogen-activated protein kinases and nuclear factor kappa B by green tea polyphenol in SKH-1 hairless mice

2003

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“…Based on several in vitro and in vivo studies, polyphenols present in green tea, most notably its major constituent epigallocatechin-3-gallate (EGCG) (Figure 1), have demonstrated remarkable antioxidant activity and photochemopreventive effects (Lu et al, 2000;Elmets et al, 2001;Kim et al, 2001;Katiyar et al 2001a, b;Katiyar and Mukhtar, 2001;Nomura et al, 2001). We demonstrated that topical application of EGCG to human skin before a single UV exposure of 4 Â minimal erythema dose markedly decreased UV-induced production of hydrogen peroxide (H 2 O 2 ) and nitric oxide in a time-dependent manner (Katiyar et al, 2001b).…”

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confidence: 99%

Inhibition of ultraviolet B-mediated activation of nuclear factor κB in normal human epidermal keratinocytes by green tea Constituent (-)-epigallocatechin-3-gallate

et al. 2003

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Epigallocatechin-3-gallate (EGCG), the major constituent of green tea, possesses significant anti-inflammatory and cancer chemopreventive properties. Studies have shown the photochemopreventive effects of green tea and EGCG in cell culture, animal models, and human skin. The molecular mechanism(s) of photochemopreventive effects of EGCG are incompletely understood. We recently showed that EGCG treatment of the normal human epidermal keratinocytes (NHEK) inhibits ultraviolet (UV)B-mediated activation of the mitogen-activated protein kinase (MAPK) pathway. In this study, we evaluated the effect of EGCG on UVB-mediated modulation of the nuclear factor kappa B (NF-jB) pathway, which is known to play a critical role in a variety of physiological functions and is involved in inflammation and development of cancer. Immunoblot analysis demonstrated that the treatment of NHEK with EGCG (10-40 lm) for 24 h resulted in a significant inhibition of UVB (40 mJ/cm 2 )-mediated degradation and phosphorylation of IjBa and activation of IKKa, in a dose-dependent manner. UVB-mediated degradation and phosphorylation of IjBa and activation of IKKa was also observed in a time-dependent protocol (15 and 30 min, 1, 2, 3, 6, 12 h post-UVB exposure). Employing immunoblot analysis, enzyme-linked immunosorbent assay, and gel shift assay, we demonstrate that EGCG treatment of the cells resulted in a significant dose-and time-dependent inhibition of UVB-mediated activation and nuclear translocation of a NF-jB/p65. Our data suggest that EGCG protects against the adverse effects of UV radiation via modulations in NF-jB pathway, and provide a molecular basis for the photochemopreventive effect of EGCG.

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“…Matrikinler, ekstraselüler matrikste mikromoleküllerin sentezini aktive ederek, kırışıklık oluşumuna karşı etkilidirler. Kırışıklık önleyici ve yüz gerdirici aktif bileşenlerdendirler 58 . Bileşimindeki palmitoil oligopeptit tek başına kozmetik bileşeni olarak temizleyici ve cilt düzenleyici iken palmitoil oligopeptit-70 emoliyan, tırnak ve cilt düzenleyici olarak belirtilmektedir 28 .…”

Section: Doğal Veya Sentetik Kökenli Peptitler Ve Türevleriunclassified

“…UV hasara karşı antieflamatuar etki gösterdikleri bu nedenle foto koruyucu etkili olarak da kullanıldıkları bilinmektedir 10,36,63 . Yapılan bir çalışmada, yeşil çay polifenollerinin eritemi azalttığı, Langerans hücrelerini sayısını düşürdüğü ve DNA hasarını azalttığı görülmüştür 58 . Sarkık sofora (Sophora japonica) ve beyaz dut (Morus alba) ağaçlarının çeşitli kısım ekstrelerinin anti-tironinaz aktiviteleri nedeniyle cilt lekelerine yönelik kozmetik olarak kullanımı belirtilmektedir 44,64 .…”

Section: Bitki Ekstreleri Ve Bileşenleriunclassified

Anti-Aging Cosmetics Approaches and Components in Products

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2016

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Cilt yaşlanması; iç veya dış faktörlerin veya bunların birlikteliğinin neden olduğu karmaşık biyolojik bir süreçtir. Gelişen üretim teknolojileri, farmasötik taşıyıcı sistemlerin kozmetik formüllerinde kullanılması ve yenilikçi bileşenlere dayalı yeni nesil kozmetik ürünler, temel kozmetik bakımdan ileri alternatifler sunmaktadır. İleri teknoloji kullanılarak üretilen kozmetik aktif bileşenleri, doğal kaynaklı hammaddelerin kozmetik bileşenleri olarak kullanımının artması ve farmasötik taşıyıcı sistemlerin kozmetik formüllerinde kullanılması ile geliştirilen kozmetik ürünler toplumların artan kozmetik beklentilerini karşılamayı hedeflemektedir. Bu derlemede, günümüzde bireylerin dış ve iç faktörler ile artan cilt yaşlanmasının, kozmetik ürün odaklı çözümleri özellikle bileşenler açısından irdelenmiş, yaşlanma karşıtı farklı yaklaşımlar özetlenmiş ve kullanılan taşıyıcı sistemler belirtilmiştir.

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Cutaneous photoprotection from ultraviolet injury by green tea polyphenols

Cited by 330 publications

References 31 publications

Suppression of UVB-induced phosphorylation of mitogen-activated protein kinases and nuclear factor kappa B by green tea polyphenol in SKH-1 hairless mice

Suppression of UVB-induced phosphorylation of mitogen-activated protein kinases and nuclear factor kappa B by green tea polyphenol in SKH-1 hairless mice

Inhibition of ultraviolet B-mediated activation of nuclear factor κB in normal human epidermal keratinocytes by green tea Constituent (-)-epigallocatechin-3-gallate

Anti-Aging Cosmetics Approaches and Components in Products

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