Cutaneous responses of cattle to extracts from Rhipicephalus appendiculatus larvae

Binta, M.G.; Cunningham, M. P.

doi:10.1016/0304-4017(84)90111-0

Cited by 14 publications

(9 citation statements)

References 6 publications

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“…There have been numerous reports which showed that naturally acquired immunity to ticks is an immune response mediated by antibodies (Willadsen, 1987;Willadsen and Kemp, 1988;Willadsen et al, 1955;Nyindo et aL, 1989;andEssuman et al, 1991, 1994); complement, (Wikel and Allen, 1977) and T-cell-dependent mechanism, associated with cutaneous hypersensitivity reactions leading to increased grooming activity (Binta and Cunnigham, 1984;Smith et al, 1989). Shapiro et al, Downloaded by [Cornell University Library] at 21:30 16 November 2014 Vol.…”

Section: Discussionmentioning

confidence: 95%

“…The expression of the immunity varies greatly depending on the type of host and the tick species involved (Rechav and Dauth, 1987). Binta and Cunnigham (1984), Brown (1985) and Smith et al, (1989), documented that the mechanism reponsible for the acquisition of immunity by zebus, Bos indicus, to Boophilus microplus and Rhipicephalus appendiculatus was partly cell-mediated. Willadsen (1987) reported that the response is mainly due to immediate hypersensitivity.…”

Section: Introductionmentioning

confidence: 97%

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Characterization ofAmblyomma variegatumtick saliva and salivary gland antigens inducing anti-tick immunity in boran cattle

Dossa

Essuman

Kaaya

1998

International Journal of Acarology

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The sequence of proteins synthesized during the feeding of Amblyomma variegatum and humoral resistance acquired by Boran cattle when exposed to A. variegatum infestations were studied. Resistance was induced by repeatedly infested cattle with all stages of the tick. Salivary gland proteins possibly responsible for the acquired immunity were studied by analysing homogenates of salivary glands (SGA) on 5-20% gradient Sodium dodecylsulphate Polyacrylamide gel electrophoresis (SDS-PAGE) and comparing them to those in the tick saliva (SA) and nymph homogenates (NH). The proteins with Mr 18, 49.5, 55, 67.5 and 160 kDs were found unique for the tick saliva. On the other hand the proteins with molecular weights of about 17, 33, 27 and 66 kDs, which seemed to have been synthesized at different times during the feeding process, were only found in SGA. When SDS-PAGE was followed with immunobloting, IgG1 subclass isolated from infestation sera recognized prominently two SGA proteins with Mr of about 58 and 53 kD and a third high molecular weight protein, less prominently. Apart from the high molecular weight protein, the rest were not recognized by the IgG2 also from the same infestation sera.

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Section: Discussionmentioning

confidence: 95%

Section: Introductionmentioning

confidence: 97%

Characterization ofAmblyomma variegatumtick saliva and salivary gland antigens inducing anti-tick immunity in boran cattle

Dossa

Essuman

Kaaya

1998

International Journal of Acarology

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“…15 Intradermal testing has long been used to analyze the antigens responsible for allergic reactions. 7,8,[10][11][12] Our results showed that the intradermal inoculation of 50 µ g of extract prepared from unfed larvae-ULE of the cattle tick Boophilus microplus in the ear of naïve European and Indian calves elicited a non-specific and immediate local inflammatory reaction characterized by swelling, heat, and redness. Even a less intense delayed type reaction cannot be ruled out.…”

Section: Discussionmentioning

confidence: 99%

Skin Test and Tick Immune Status in Susceptible and Resistant Cattle in Brazil

Bechara

Morelli

Szabó

2000

Annals of the New York Academy of Sciences

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A cutaneous hypersensitivity test (CHT) was used to correlate host resistance to ticks and type of reaction elicited to unfed larval extract‐ULE of the cattle tick Boophilus microplus in European and Indian cattle. Twenty calves were separated into four groups of five animals each: naïve or preinfested Indian or European cattle. CHT was induced by intradermal inoculation of 0.1 ml of ULE cattle tick B. microplus (50 μg protein) in the calf ear. Ear thickness was measured using calipers before and 10 min, 1, 2, 6, 18, 24, 48, 72, 96, and 144 h postinoculation (PI). Preinfested European calves showed only an immediate type reaction with maximum response (75% increase in ear thickness) at 10 min PI. On the other hand, preinfested Indian calves presented an immediate response with maximum reaction (70% increase in ear thickness) between 10 min and one hour PI, and a delayed type reaction at 72 h PI (60% increase in ear thickness). These results point out the crucial role of the cellular immune response of cattle in the expression of resistance to cattle tick B. microplus. Skin test might be useful in the ranking of cattle according to the susceptibility/resistance to ticks.

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“…Willadsen et al 21 used purified antigens from whole larvae of Boophilus microplus (Canestrini, 1887) and found that skin reactions correlated well with the levels of host resistance measured by tick feeding. Binta and Cunningham 5 observed that an extract of Rhipicephalus appendiculatus (Neumann, 1901) larvae induced an immediate hypersensitivity reaction in sensitised cattle following intradermal inoculation. Generally, the extent of cutaneous reaction depends upon duration of exposure of the cattle to tick infestation.…”

Section: Discussionmentioning

confidence: 99%

Cutaneous hypersensitivity induced in rabbits by extracts of the tick Amblyomma cajennense (Acari : Ixodidae)

Hlatshwayo¹,

Szabó²,

Bechara³

et al. 2004

J. S. Afr. Vet. Assoc.

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The cutaneous hypersensitivity test was used to correlate host resistance to ticks and type of reactions elicited by Amblyomma cajennense (Fabricius, 1787) tick extract in rabbits. Rabbits were divided into 3 groups of 2 animals each: naive, pre-infested and control. Cutaneous hypersensitivity was induced by intradermal inoculation of 25 mg extract in 0.03 mℓ of phosphate buffered saline (PBS) in rabbit ears. Control rabbits were inoculated with PBS only. The ear thickness was measured with a Mitutoyo (R) device before and 10 min, 1, 2, 4, 18, 24, 48, 72 and 96 h post-inoculation (PI). Pre-infested rabbits showed an immediate type reaction within the 1st 10 min PI (60%increase in ear thickness) and a delayed reaction (18 h) (85%increase), whereas the naive rabbits showed only the immediate reaction within the 1st 4 h (60%increase). PBS induced only mild reactions. These results point out the crucial role of the cellular immune response of rabbits in the expression of resistance to A. cajennense.

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Cutaneous responses of cattle to extracts from Rhipicephalus appendiculatus larvae

Cited by 14 publications

References 6 publications

Characterization ofAmblyomma variegatumtick saliva and salivary gland antigens inducing anti-tick immunity in boran cattle

Characterization ofAmblyomma variegatumtick saliva and salivary gland antigens inducing anti-tick immunity in boran cattle

Skin Test and Tick Immune Status in Susceptible and Resistant Cattle in Brazil

Cutaneous hypersensitivity induced in rabbits by extracts of the tick Amblyomma cajennense (Acari : Ixodidae)

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