Cutaneous Sarcoma-like Lesions of the Horse Caused by the Agent of Bovine Papilloma.

Olson, Carl; Cook, R. H.

doi:10.3181/00379727-77-18750

Cited by 120 publications

(75 citation statements)

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“…Subsequently, more substantial evidence was provided by transmission studies in which inoculation with either sarcoid tissue or cell-free supernatant from minced tumours onto the scarified skin of sarcoid-free horses resulted in the appearance of tumours at the inoculation site; these sarcoids were morphologically indistinguishable from naturally occurring sarcoids (Voss, 1969). Similarly, inoculation with bovine papillomavirus (BPV) in nonaffected horses resulted in the growth of sarcoid-like tumours (Olson & Cook, 1951;Ragland & Spencer, 1969). However, the artificially induced sarcoids showed spontaneous regression, which is rarely encountered in naturally occurring sarcoid tumours.…”

Section: Evidence For Virus Aetiologymentioning

confidence: 99%

Association of bovine papillomavirus with the equine sarcoid

Chambers

Ellsmore

O’Brien

et al. 2003

Journal of General Virology

187

180

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The equine sarcoid, a locally aggressive, fibroblastic skin tumour, is the most common dermatological neoplasm reported in horses; there is no consistently effective therapy. It is widely accepted that bovine papillomavirus (BPV) types 1 and 2 are associated with the pathogenesis of sarcoid disease. Most sarcoids appear to contain detectable viral DNA and RNA and are also known to express the BPV types 1 and 2 major transforming protein, E5, but appear not to produce infectious virions. While the mode of transmission of infection has not been elucidated, viral gene expression, in particular of E5, may contribute to virus persistence and disease pathogenesis by downregulating MHC class I expression. Here, the pathology and epidemiology of the sarcoid and its association with BPV is reviewed; the transforming functions of the BPV oncoproteins and their possible role in sarcoid pathogenesis are discussed; and the practical implications of BPV infection for diagnostic and therapeutic purposes are considered.

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Section: Evidence For Virus Aetiologymentioning

confidence: 99%

Association of bovine papillomavirus with the equine sarcoid

Chambers

Ellsmore

O’Brien

et al. 2003

Journal of General Virology

187

180

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“…Inoculation of BPV into horses and hamsters can lead to the formation of turnouts. In hamsters the tumours often metastasize (Robl & Olson, 1968), while in horses BPV induces a connective tissue turnout histologically similar to equine sarcoid (Olson & Cook, 1951). Virus particles are not found in BPV-induced tumours but multiple copies of BPV-specific DNA sequences can be detected by DNA-DNA hybridization analysis (Lancaster et aL, 1976(Lancaster et aL, , 1977.…”

Section: Introductionmentioning

confidence: 99%

Isolation and Characterization of the Major Capsid Protein of Bovine Papilloma Virus Type 1

Meinke

1981

Journal of General Virology

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SUMMARYThe major capsid protein of bovine papilloma virus type 1 (BPV-1) was isolated by gel filtration following disruption of purified virus particles with guanidine hydrochloride. The capsid protein, VP1, has a mol. wt. of about 53 500. Amino acid composition studies of VP1 showed that it is a highly acidic protein containing almost twice the average number of acidic residues than basic residues. Relatedness was observed between VP1 and the major capsid proteins of simian virus 40 (SV40) and polyoma virus.

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“…26,29,34 In subsequent work, Southern blots were used to identify BPV1 or BPV2 genome(s) within tumor tissue, 1,2,19,38 and polymerase chain reaction (PCR)-based surveys routinely demonstrate that up to 100% of sarcoids contain BPV DNA. [12][13][14]23,24,27,38,39 A limited number of studies have been designed to investigate the role of BPV in cellular transformation in equine tissue.…”

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Localization of Bovine Papillomavirus Nucleic Acid in Equine Sarcoids

et al. 2015

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Bovine papillomaviruses (BPV1/BPV2) have long been associated with equine sarcoids; deciphering their contribution has been difficult due to their ubiquitous presence on skin and in the environment, as well as the lack of decent techniques to interrogate their role in pathogenesis. We have developed and characterized an in situ hybridization (ISH) assay that uses a pool of probes complementary to portions of the E5, E6, and E7 genes. This assay is highly sensitive for direct visualization of viral transcript and nucleic acid in routinely processed histopathologic samples. We demonstrate here the visualization of BPV nucleic acid in 18 of 18 equine sarcoids, whereas no detectable viral DNA was present in 15 of 15 nonsarcoid controls by this technique. In nearly 90% (16/18) of the sarcoids, 50% or more of the fibroblastic cell nuclei distributed throughout the neoplasm had detectable hybridization. In the remaining 2 cases, fewer than half of the fibroblastic cells contained detectable hybridization, but viral nucleic acid was also detected in epithelial cells of the sebaceous glands, hair follicles and epidermis. A sensitive ISH assay is an indispensable addition to the molecular methods used to detect viral nucleic acid in tissue. We have used this technique to determine the specific cellular localization and distribution of BPV in a subset of equine sarcoids.

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Cutaneous Sarcoma-like Lesions of the Horse Caused by the Agent of Bovine Papilloma.

Cited by 120 publications

References 0 publications

Association of bovine papillomavirus with the equine sarcoid

Association of bovine papillomavirus with the equine sarcoid

Isolation and Characterization of the Major Capsid Protein of Bovine Papilloma Virus Type 1

Localization of Bovine Papillomavirus Nucleic Acid in Equine Sarcoids

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