Cutaneous wound healing activity of herbal ointment containing the leaf extract of <i>Acalypha indica L</i>. on mice (<i>Mus musculus</i>)

Laut, Meity Marviana; Ndaong, Nemay; Utami, Tania Surya

doi:10.1088/1742-6596/1146/1/012025

Cited by 9 publications

(4 citation statements)

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“…11,35 A.indica was reported to increase the rate of wound contraction and faster re-epithelialization compared to betadine ointment used as positive control. 36 Bacopa monnieri, Acalypha indica and Calotropis gigantea were selected for investigating their combinatorial efficacy on the architectural changes in the histology of wound tissue post healing in comparison with that of the individual extract treatment groups. Changes with respect to skin tissue histology, epidermal differentiation, epidermal & dermal thickness, cell proliferation tendency, angiogenesis and collagen type I remodeling were assessed using suitable assays.…”

Section: Discussionmentioning

confidence: 99%

Dermal wound healing contribution of aqueous extracts ofAcalypha indica, Calotropis gigantea, Bacopa monnieriand their combination

Tanimu,

Zirmire K,

Jamora

et al. 2024

Preprint

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Wound healing is a complex process that requires a well-orchestrated integration of an array of molecular events such as cell migration and proliferation, deposition and remodeling of extracellular matrix components for restoring the structural and functional integrity of the tissue injured. Ayurveda suggests wound healing herbs can achieve enhanced therapeutic effect with reduced toxicity when they are optimally combined in a specific ratio as polyherbal formulation (PHF). The present study was aimed to evaluate the combinatorial wound healing efficacy (invivowound closure and histological changes) of aqueous extracts of three medicinal plants (Bacopa monnieri,Acalypha indicaandCalotropis gigantea). This study also explored how the combination influenced the overall quality of healed wound. Individual wound closure kinetic performance of aqueous plant extracts in C57B/6J mice was assessed using safe concentrations obtained from human adult dermal fibroblast viability assay. The aqueous plant extract combination optimized using response surface methodology was tested forinvivowound closure effectiveness. Quality of healed wound was assessed via Hematoxylin & Eosin and immunohistochemical staining of markers (K1, K5, Loricrin, Ki67, CD31 and collagen1). The combination treatment (B.monnieri-15μg/ml,A.indica-11.59μg/ml,C.gigantea-1μg/ml) contributed to faster wound closure (11 days), improved collagen type I remodeling and angiogenesis, complete re-epithelialization, similar epidermal differentiation pattern as that of individual and control treatments. Ki67 staining revealed no significant increase in cell proliferation in combination compared to individual and control. Findings from the study validates the polyherbal combination′s impressive capability to promote wound healing.

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Section: Discussionmentioning

confidence: 99%

Dermal wound healing contribution of aqueous extracts ofAcalypha indica, Calotropis gigantea, Bacopa monnieriand their combination

Tanimu,

Zirmire K,

Jamora

et al. 2024

Preprint

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“…Acalypha wilkesiana has been used in various countries for management of different topical conditions. Laut et al, 17 developed medicated ointment containing extract of A. indica leaves obtained from Indonesia which exhibited wound healing process in the incision wound models by enhanced wound contraction and shortened epithelization period. Fawehinmi et al, 18 showed that Acalypha wilkesiana ethanol extract obtained from Nigeria, has high potential as an anti-dermatophyte agent when formulated as cream for topical application.…”

Section: Antimicrobial Activity Screeningmentioning

confidence: 99%

Evaluation of the Antimicrobial Effectiveness of Topical Gels Containing Acalypha wilkesiana

2021

TJNPR

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Staphylococcus aureus. 4.5 It has also been used as a cardioprotective agent as well as in the management of diabetes. 6 Other reported uses of A. wilkesiana include treatment of cold, headache, malaria 4 , inflammation and tumours in the breast. 6 Most publications on A. wilkesiana are based on the investigation of its pharmacological action or the support of its use in traditional health through phytochemical analysis of the aqueous, ethanolic or powder extracts. To the best of our knowledge, apart from Oyelami et al, who investigated the efficacy and safety of Acalypha wilkesiana ointment in the treatment of superficial fungal skin infection 2 , none have investigated the formulation of this medicinally active plant into a dosage form for optimized dosing and therapeutic effect. In this study, the antimicrobial (antibacterial and antifungal) properties of A. wilkesiana were determined, the most appropriate gel dosage form for its therapeutic effectiveness was investigated and the effective strength in the dosage form ascertained through minimum inhibitory and zone of inhibition studies. Materials and Methods Solvents and ReagentsTween 80 was purchased from BDH Chemicals, England. Clotrimazole powder BP (> 98%) purity and Levofloxacin powder BP (>98%) were obtained from Drugfield Pharmaceutical company, Ogun State, Nigeria. Carbopol 940 (Shree organics, India), methylparaben, propyl paraben (Sigma-

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“…For preparing extract formulations, 1.75g (3.5%), 2.5g (5%), 5g (10%) of the extract mixed with 48.25g, 47.5g, 45g white Vaseline, respectively. All ingredients of leaves extract ointment were mixed in the mortar and stirring constantly until homogenous and form an ointment preparation, and put in a clean container for topical application during the experiment (Laut et al, 2019).…”

Section: Ointment Preparationmentioning

confidence: 99%

Wound healing activities of Moringa oleifera leaves extract cultivated in Kurdistan region-Iraq

2021

JJBS

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Medicinal plants have been used for many years as an ancient curative method for treating and healing wounds in different cultures. Therefore, and accordingly this work has been conducted to study wound healing activity of Moringa oleifera leaves extract cultivated in the Kurdistan region in the northern Iraq (KRG). In the current investigation, experimentally-induced wounds in rats have been infected with strains of Staphylococcus aureus and Pseudomonas aeruginosa clinically isolated from the wound site in hospitalized patients. High Performance Liquid Chromatography (HPLC) is used to determine some bioactive compounds within the extract. Plant leaf was extracted by using the maceration method and 70% ethanol as solvent. The HPLC results were dependent on comparison between the extract with standards. Two lacerated wounds were made on each animal at either side of the thoracolumbar spine and inoculated by a 0.4 ml bacterial suspension. The treatment regimen was for 14 days with different formulation of ethanolic leaves extract, and gentamicin ointment as a control positive. At the end point of the experimental trial, animals were euthanized humanly at day 15. Samples from healed-wounded skin was prepared for histological evaluation. Generally speaking, our findings indicated that alcoholic leaf extract showed potential wound healing property in different concentration as a dose-dependent manner of the extracted ointment 3.5%, 5%,10% particularly 10% of the extract formulation which showed better results in comparison to gentamicin ointment. The presented essential constituents for Moringa leaf derivatives were gallic acid (3461 ppm), catechin (1201 ppm), rutin (286 ppm) and quercetin (88 ppm). Last but not least, the extract was able to provide promising evidence to possess a drug formulation material.

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Cutaneous wound healing activity of herbal ointment containing the leaf extract of Acalypha indica L. on mice (Mus musculus)

Cited by 9 publications

References 1 publication

Dermal wound healing contribution of aqueous extracts ofAcalypha indica, Calotropis gigantea, Bacopa monnieriand their combination

Dermal wound healing contribution of aqueous extracts ofAcalypha indica, Calotropis gigantea, Bacopa monnieriand their combination

Evaluation of the Antimicrobial Effectiveness of Topical Gels Containing Acalypha wilkesiana

Wound healing activities of Moringa oleifera leaves extract cultivated in Kurdistan region-Iraq

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