Cutaneous yeast microflora in patients with atopic dermatitis

Abstract

<jats:title>Abstract</jats:title><jats:p>The skin of persons with atopic dermatitis (AD) is very susceptible to cutaneous infection, and some yeast species may also aggravate AD. The total yeast population of an AD patient’s skin and its relation with individual age and body part remains poorly characterized. The aim of this study was to clarify the differences in cutaneous yeast flora by age and body parts of AD patients.</jats:p><jats:p>By swabbing affected body parts (hands, legs, face, neck or trunk), 241 samples were collected from patients with AD (132 children and 109 adults), and as controls, 40 samples were taken from healthy individuals (20 children, 20 adults).</jats:p><jats:p>In all, 89 (36.9%) of samples were positive; the yeast isolated belonged to three genera: Candida (27.4%), Malassezia (6.6%), and Rhodotorula (2.9%). Cutaneous colonization with yeasts was two-fold higher in the adults than in children (P&lt;0.0001). The distribution of the yeast species was dependent on the body part sampled: Malassezia predominated in the face, neck, and trunk regions (P=0.0047); Candida more frequently colonized hands and legs (P=0.0029).</jats:p><jats:p>Our study showed that cutaneous yeast flora and distribution of yeast species depends significantly on the age of the AD patient and the body part affected by atopic dermatitis.</jats:p>

Pulsed Electromagnetic Field Assisted in vitro Electroporation: A Pilot Study

“…Saccharomyces cerevisiae α’1 was obtained from the collection of microorganisms of Nature Research Centre (Lithuania). C. lusitaniae was isolated from the skin of the patient with clinical diagnosis of atopic dermatitis and identified applying assimilation test api ID32C (bioMerieux sa, France) 65 . Informed written consent was obtained from all subjects.…”

Atypical non-lipid-dependent strains of Malassezia furfur

“…M. furfur has been described as showing high genotypic variability, and these specific genotypes could be related to geographic origin of the isolates, skin disease origin, age groups, body sites from which they come, or the host (human or animal associated subtypes) [43][44][45][46]. In Lithuania, cutaneous colonization with Malassezia species is very low as compared with studies from other countries [9,[47][48][49]. The cutaneous microenvironment is generally considered to be important for Malassezia populations.…”

Growth Inhibition and Membrane Permeabilization ofCandida lusitaniaeUsing Varied Pulse Shape Electroporation

“…The study was performed on Candida lusitaniae strain CL18 isolated from the skin of patients with clinical diagnosis of atopic dermatitis [ 27 , 28 ]. Yeast cells were grown on the rich standard YPD medium (2% glucose, 2% peptone, 1% yeast extract, and 1% agar) for 48 hours at 30°C.…”