Cutibacterium acnes molecular typing: time to standardize the method

Dagnelie, Marie‐Ange; Khammari, Amir; Dréno, Brigitte; Corvec, Stéphane

doi:10.1016/j.cmi.2018.03.010

Cited by 32 publications

(22 citation statements)

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“…Cutibacterium acnes is an important member of the human skin microbiota (Christensen & Brüggemann, 2014). Although the taxonomy of this species was recently revised (Scholz & Kilian, 2016), C. acnes remains heterogeneous and has been sub-divided in six principal phylotypes (Dagnelie et al, 2018). In skin, bacteria such as the RT4 HL045PA1/HM-516 (phylotype IA 1 ) appear predominantly associated to acne, whereas strains such as the RT6 HL110PA3/ HM-554 (Phylotype II) are found on healthy skin areas (Fitz-Gibbon et al, 2013).…”

Section: Discussionmentioning

confidence: 99%

“…However, this species remains itself highly heterogeneous with six main phylotypes IA1, IA2, IB, IC, II, and III (Dagnelie, Khammari, Dréno, & Corvec, 2018;McDowell, 2017). Cutibacterium acnes genome is characterized by a large stable core (>80% of chromosomal DNA) whereas 7%-11% of coding sequences should be species or even strain specific (Brzuszkiewicz et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

“…Cutibacterium acnes , usually known as Proprionibacterium acnes , was recently renamed in order to distinguish this high GC content and human skin associated bacterium from other related species (McDowell, Barnard, Liu, Li, & Patrick, ; Sholz & Kilian, ). However, this species remains itself highly heterogeneous with six main phylotypes IA1, IA2, IB, IC, II, and III (Dagnelie, Khammari, Dréno, & Corvec, ; McDowell, ). Cutibacterium acnes genome is characterized by a large stable core (>80% of chromosomal DNA) whereas 7%–11% of coding sequences should be species or even strain specific (Brzuszkiewicz et al, ).…”

Section: Introductionmentioning

confidence: 99%

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Adaptation of acneic and non acneic strains of Cutibacterium acnes to sebum‐like environment

et al. 2019

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Cutibacterium acnes, former Proprionibacterium acnes, is a heterogeneous species including acneic bacteria such as the RT4 strain, and commensal bacteria such as the RT6 strain. These strains have been characterized by metagenomic analysis but their physiology was not investigated until now. Bacteria were grown in different media, brain heart infusion medium (BHI), reinforced clostridial medium (RCM), and in sebum like medium (SLM) specifically designed to reproduce the lipid rich environment of the sebaceous gland. Whereas the RT4 acneic strain showed maximal growth in SLM and lower growth in RCM and BHI, the RT6 non acneic strain was growing preferentially in RCM and marginally in SLM. These differences were correlated with the lipophilic surface of the RT4 strain and to the more polar surface of the RT6 strain. Both strains also showed marked differences in biofilm formation activity which was maximal for the RT4 strain in BHI and for the RT6 strain in SLM. However, cytotoxicity of both strains on HaCaT keratinocytes remained identical and limited. The RT4 acneic strain showed higher inflammatory potential than the RT6 non acneic strain, but the growth medium was without significant influence. Both bacteria were also capable to stimulate β‐defensine 2 secretion by keratinocytes but no influence of the bacterial growth conditions was observed. Comparative proteomics analysis was performed by nano LC‐MS/MS and revealed that whereas the RT4 strain only expressed triacylglycerol lipase, the principal C. acnes virulence factor, when it was grown in SLM, the RT6 strain expressed another virulence factor, the CAMP factor, exclusively when it was grown in BHI and RCM. This study demonstrates the key influence of growth conditions on virulence expression by C. acnesand suggest that acneic and non acneic strains are related to different environmental niches.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Adaptation of acneic and non acneic strains of Cutibacterium acnes to sebum‐like environment

et al. 2019

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“…The reclassification from Propionibacterium acnes to Cutibacterium acnes was proposed in 2016 . A standardized method to perform molecular typing of C. acnes , according to the degree of resolution needed (phylotypes, clonal complexes, single‐locus sequence typing), has been recently suggested …”

mentioning

confidence: 99%

“…1 A standardized method to perform molecular typing of C. acnes, according to the degree of resolution needed (phylotypes, clonal complexes, single-locus sequence typing), has been recently suggested. 2 In the first article of this supplement, the authors review the most recent data on C. acnes and its involvement in etiopathogenesis of acne. 3 New findings on C. acnes have revealed that, contrary to what was previously thought, its proliferation is not the trigger of acne.…”

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Two important novelties in etiopathogenesis and therapy of acne

Veraldi

2018

Acad Dermatol Venereol

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Cutibacterium and Staphylococcus dysbiosis of the skin microbiome in acne and its decline after isotretinoin treatment

Feidenhansl,

Lund,

Poehlein

et al. 2024

JEADV Clinical Practice

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BackgroundAcne vulgaris is a multifactorial disease of the pilosebaceous unit of human skin. Previous studies have identified an acne‐associated dysbiosis of the skin microbiome.ObjectivesThis dysbiosis was mainly determined for Cutibacterium acnes. However, detailed analyses combining qualitative and quantitative aspects are scarce, also regarding the possible contribution of other skin bacteria and the impact of treatment.MethodsWe conducted a culture‐independent study to determine differences between the healthy skin and the acne microbiome before and after isotretinoin treatment. Three amplicon‐based sequencing approaches and digital droplet PCR for quantification were applied.ResultsOur results revealed a 2.2‐fold reduced abundance of C. acnes with a reduced diversity in the acne microbiome. A phylotype switch was found, which was mainly characterized by a significant relative decrease of IB and II strains in the acne microbiome. In contrast, the relative abundance of staphylococci increased significantly and the quantitative ratio of staphylococci to C. acnes strongly increased from 1:34 in the healthy cohort to 1:11 in the acne cohort. The diversity of staphylococci was reduced, mainly due to the decrease of Staphylococcus hominis, and the appearance and predominance of Staphylococcus aureus in some acne patients. Isotretinoin treatment drastically depleted C. acnes (37‐fold) and moderately also staphylococci (3.6‐fold). Isotretinoin treatment resulted in a decrease of Staphylococcus epidermidis and a significant increase of S. aureus on facial skin.ConclusionsThe switch from a C. acnes‐dominated healthy skin microbiome towards an acne microbiome that is relatively enriched in staphylococci could indicate a stronger impact of staphylococci in the pathophysiology of acne than currently acknowledged. Our data further showed that isotretinoin largely eliminated the skin microbiome and in particular C. acnes, but also S. epidermidis. Instead, more harmful bacteria such as S. aureus could expand, suggesting that posttreatment strategies should be considered to accelerate skin microbiome recovery.

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Cutibacterium acnes molecular typing: time to standardize the method

Abstract: There is an obvious need to create a consensus about molecular typing methods for C. acnes. This standardization will facilitate the comparison of results between one article and another, and also the interpretation of clinical data.

Cited by 32 publications

References 45 publications

Adaptation of acneic and non acneic strains of Cutibacterium acnes to sebum‐like environment

Adaptation of acneic and non acneic strains of Cutibacterium acnes to sebum‐like environment

Two important novelties in etiopathogenesis and therapy of acne

Cutibacterium and Staphylococcus dysbiosis of the skin microbiome in acne and its decline after isotretinoin treatment

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