Cuticular protein gene LmACP8 is involved in wing morphogenesis in the migratory locust, Locusta migratoria

Zhao, Xiaoming; Yang, Jiapeng; Gou, Xin; Liu, Weimin; Zhang, Jianzhen

doi:10.1016/s2095-3119(20)63248-8

Cited by 7 publications

(8 citation statements)

References 38 publications

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“…In this study, we found that LmACP19 was mainly expressed in the pre-ecdysis based on our transcriptome data and RT-qPCR analysis ( Figure 1 A–A′ and Figure 2 B), which is similar to that of the other two wing cuticle protein genes LmACP7 and LmACP8 that also encoded a member of subgroup RR-2 of the CPR family. We previously confirmed that LmACP7 protein accumulates in the exocuticle, while LmACP8 is located in the exocuticle and endocuticle of wing pads and adult wings [ 19 , 20 ]. Compared to LmACP7 and LmACP8, immunohistochemistry revealed that LmACP19 only was expressed in the epidermal cells of wing pads during wing development ( Figure 2 C), implying that LmACP19 may play different physiological functions in wing cuticle development than LmACP7 and LmACP8.…”

Section: Discussionmentioning

confidence: 59%

“…Compared to LmACP7 and LmACP8, immunohistochemistry revealed that LmACP19 only was expressed in the epidermal cells of wing pads during wing development ( Figure 2 C), implying that LmACP19 may play different physiological functions in wing cuticle development than LmACP7 and LmACP8. In our previous work, suppressing LmACP7 or LmACP8 expression by RNAi only resulted in wing defects during nymph–adult transition, and the resulting adult wings were not fully elongated but wrinkled [ 19 , 20 ]. Here, injection of ds LmACP19 into fourth- and fifth-instar nymphs resulted in wing pads and wing defects during nymph–nymph transition and nymph–adult transition, respectively.…”

Section: Discussionmentioning

confidence: 99%

“…We previously demonstrated that dysfunction of LmACP7 or LmACP8 resulted in disordered lamellae of the exocuticle and the thickness of the endocuticle was dramatically decreased during wing development [ 19 , 20 ]. Similarly, we also found that the thickness of the endocuticle was dramatically decreased during wing development after suppressing LmACP19 from the ultrastructure level, but not the exocuticle ( Figure 5 ), suggesting that LmACP19 is required for structural integrity of the wing endocuticle during molting.…”

Section: Discussionmentioning

confidence: 99%

“…Among them, LmACP7 is an indispensable structural component needed for wing morphological and structural integrity of wing cuticle in the migratory locust [ 19 ]. LmACP8 is negatively regulated by the hormone receptor LmHR39-mediated 20-hydroxyecdysone signaling pathway and is involved in wing development during nymph to adult transition [ 20 ]. It is noteworthy that LmACP7 accumulates in the exocuticle, while LmACP8 is located in the exocuticle and endocuticle of wing pads and adult wings and co-locates with chitin.…”

Section: Introductionmentioning

confidence: 99%

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Cuticle Protein LmACP19 Is Required for the Stability of Epidermal Cells in Wing Development and Morphogenesis of Locusta migratoria

Zhao

Shao

et al. 2022

IJMS

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Insect wing consists of a double layer of epidermal cells that produce and secrete the dorsal and ventral cuticular components. It is important for the stability of epidermal cells during wing development and morphogenesis, but its specific gene expression and physiological function during this process remain unclear. In our previous work, a wing cuticle protein gene LmACP19 was identified in Locusta migratoria based on transcriptomic data. Here, we report on its roles in wing development and morphogenesis. LmACP19 encodes a chitin-binding protein belonging to RR-2 subfamily of CPR family, which is highly homologous to CP19-like proteins in other insect species. RT-qPCR analysis revealed that LmACP19 is highly expressed in wing pads of fifth-instar nymphs, and its encoded protein is located in two layers of epidermal cells but not in the cuticle. Suppression of LmACP19 by RNA interference led to abnormal wing pad and wing morphogenesis with curved, unclosed, and wrinkled phenotypes during nymph-to-nymph and nymph-to-adult transition, respectively. Furthermore, deficiency of LmACP19 affected arrangement of epidermal cells, resulting in apoptosis. Our results indicate that LmACP19 is indispensable for wing development and normal morphological structure by maintaining the stability of epidermal cells during L. migratoria molting.

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Section: Discussionmentioning

confidence: 59%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Cuticle Protein LmACP19 Is Required for the Stability of Epidermal Cells in Wing Development and Morphogenesis of Locusta migratoria

Zhao

Shao

et al. 2022

IJMS

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“…P450, CCE, ecdysteroid kinase-like), ANK, histone-related genes and cuticle protein which are major components of the insect cuticle-associated organs such as integument and wings. 94 …”

Section: Resultsmentioning

confidence: 99%

A chromosome-level genome assembly of the pollinating fig wasp Valisia javana

et al. 2022

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Fig wasp has always been thought the species-specific pollinator for their host fig (Moraceae, Ficus) and constitute a model system with its host to study co-evolution and co-speciation. The availability of a high-quality genome will help to further reveal the mechanisms underlying these characteristics. Here, we present a high-quality chromosome-level genome for Valisa javana developed by a combination of PacBio long-read and Illumina short-read. The assembled genome size is 296.34 Mb from 13 contigs with a contig N50 length of 26.76 kb. Comparative genomic analysis revealed expanded and positively selected genes related to biological features that aid fig wasps living in syconium of its highly specific host. Protein-coding genes associated with chemosensory, detoxification and venom genes were identified. Several differentially expressed genes in transcriptome data of V. javana between odor-stimulated samples and the controls have been identified in some olfactory signal transduction pathways, e.g. olfactory transduction, cAMP, cGMP-PKG, Calcim, Ras and Rap1. This study provides a valuable genomic resource for a fig wasp, and sheds insight into further revealing the mechanisms underlying their adaptive traits to their hosts in different places and co-speciation with their host.

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Development and formation of wing cuticle based on transcriptomic analysis in Locusta migratoria during metamorphosis

Zhang,

Wu,

et al. 2024

Journal of Integrative Agriculture

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Cuticular protein gene LmACP8 is involved in wing morphogenesis in the migratory locust, Locusta migratoria

Cited by 7 publications

References 38 publications

Cuticle Protein LmACP19 Is Required for the Stability of Epidermal Cells in Wing Development and Morphogenesis of Locusta migratoria

Cuticle Protein LmACP19 Is Required for the Stability of Epidermal Cells in Wing Development and Morphogenesis of Locusta migratoria

A chromosome-level genome assembly of the pollinating fig wasp Valisia javana

Development and formation of wing cuticle based on transcriptomic analysis in Locusta migratoria during metamorphosis

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