Cutinase production by Fusarium oxysporum in liquid medium using central composite design

Abstract

The objective of the present study was to measure the production of cutinase by Fusarium oxysporum in the presence of several carbon and nitrogen sources (glycides, fatty acids and oils, and several organic and inorganic nitrogen sources), trying to find a cost-effective substitute for cutin in the culture medium as an inducer of cutinase production. The results were evaluated by the Tukey test, and flaxseed oil was found to give the best results as a cutinase inducer. The authors optimized the composition of the growth medium employing response surface methodology. The experimental results were fitted to a second-order polynomial model at a 95% level of significance (p < 0.05). The greatest cutinolytic activity was obtained in a liquid mineral medium supplemented with flaxseed oil, showing an increase in enzymatic activity from 11 to 22.68 U/mL after 48 h of fermentation. A CCD study of the fermentation conditions was carried out, and the best production of cutinase was registered with the use of 30 degrees C and 100 rpm. These results support the use of flaxseed oil as a substitute for cutin, which is difficult and expensive to obtain, for the production of cutinase in a larger scale.

Three New Cutinases from the Yeast Arxula adeninivorans That Are Suitable for Biotechnological Applications

“…The products were found to be identical, indicating that these four cutinases have the same cutinolytic activity. In previous work, it has been shown that the major components released by enzymatic hydrolysis of cutin are 9,10,8-trihydroxy-C 18 , 10,16-dihydroxy-C 16 , and -hydroxy-C 16 and C 18 with R f values of 0.34, 0.51, and 0.72, respectively (39,50). Three of the spots visible in Fig.…”

“…The first cutinases were found in fungi of the genus Fusarium (16)(17)(18)(19)(20). Recently, a low-molecular-mass cutinase from the fungus Thielavia terrestris that is able to efficiently hydrolyze polyesters has been reported by Yang et al (5).…”

Enhanced extracellular expression of gene-optimized Thermobifida fusca cutinase in Escherichia coli by optimization of induction strategy

“…The optimization of cutinase production from these wild-type strains has been widely studied in the past decades. Reports have shown that the yield of cutinase is influenced by both the composition of the medium and culture conditions [8][9][10][11]. Certain additives, including cutin, cutin hydrolysis monomers, and some lipids can be used as inducers to improve the production of cutinase [9,12].…”

Modeling and optimization of cutinase production by recombinant Escherichia coli based on statistical experimental designs

“…In this regard, several strategies such as strain development, culture condition optimization, and mathematical modeling have been widely used. For a certain biotechnological process, developing an appropriate culture medium is of crucial importance because the culture medium can significantly affect product yield [23]. The traditional method of optimization involves varying one factor at a time, while keeping the other constant.…”

“…In this respect, fractional factorial design and response surface analysis are important tools to determine optimal process conditions. The statistics-based experimental designs for optimization have been used in many areas of biotechnology such July, 2010 as optimization of a culture medium [23], enzyme production [11], ethanol production [24], and biomass production [25].…”