CXCL10 treatment promotes reduction of IL-10+ regulatory T (Foxp3+ and Tr1) cells in the spleen of BALB/c mice infected by Leishmania infantum

Figueirêdo, Webertty Mayk Eufrásio de; Heredia, Fabiola Fernandes; Santos, Aline Sombra; Braga, Roberta da Rocha; Fonseca, Francisco Rafael Marciano; Rodrigues, Naya Lúcia de Castro; Abreu, Ticiana Monteiro; Pompeu, Margarida Maria de Lima; Barbosa, Helene Santos; Teixeira, Maria Jânia

doi:10.1016/j.exppara.2019.107789

Cited by 19 publications

(13 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Early reports identified a requirement for IFNγ-priming of macrophages and for their ability to produce TNF to control parasite growth [60] . In line with these reports, c-x-c motif chemokine ligand (CXCL)10 treatment was associated with a negative correlation between IL-10 and Tr1 cells [61] . Consequently, this resulted in reduced parasite burdens and splenomegaly but did not affect the ratio of white pulp area to total spleen area [61] .…”

Section: Introductionsupporting

confidence: 61%

“…In line with these reports, c-x-c motif chemokine ligand (CXCL)10 treatment was associated with a negative correlation between IL-10 and Tr1 cells [61] . Consequently, this resulted in reduced parasite burdens and splenomegaly but did not affect the ratio of white pulp area to total spleen area [61] . Since CXCL10 induces IFNγ secretion, it was suggested that improved macrophage activation contributed to parasite control as well as maintenance of the splenic architecture [61] .…”

Section: Introductionsupporting

confidence: 61%

“…Consequently, this resulted in reduced parasite burdens and splenomegaly but did not affect the ratio of white pulp area to total spleen area [61] . Since CXCL10 induces IFNγ secretion, it was suggested that improved macrophage activation contributed to parasite control as well as maintenance of the splenic architecture [61] . A transcriptional profiling study of the spleen during VL in the hamster model revealed that the IFNγ response did not restrain parasite growth or disease, supporting the accumulating evidence that macrophages are ineffectively activated to kill the parasite [62] .…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Cytokines and splenic remodelling during Leishmania donovani infection

Engwerda

Kaye

2020

Cytokine: X

View full text Add to dashboard Cite

Highlights Visceral leishmaniasis leads to extensive remodelling of splenic microarchitecture. Splenomegaly is associated with compartmentalised vascular remodelling. Alterations in white pulp stromal cells affects leucocyte segregation. Splenic remodelling involves multiple cytokines from diverse cellular sources. Understanding splenic remodelling may lead to new therapeutic interventions.

show abstract

Section: Introductionsupporting

confidence: 61%

Section: Introductionsupporting

confidence: 61%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Cytokines and splenic remodelling during Leishmania donovani infection

Engwerda

Kaye

2020

Cytokine: X

View full text Add to dashboard Cite

show abstract

“…These two chemokines were strongly upregulated at 1 dpi (Figure 11-figure supplement 1) and they are critical in both innate and adaptive immune responses to Leishmania infection (27). CXCL10 has been studied as a treatment in BALB/c mice infected by L. infantum, since there is evidence that CXCL10-treatment reduces IL-10 + Treg cell populations (CD4 + CD25 + Foxp3 + and Tr1) and induces morphological changes in the spleen (28). This chemokine also induces a reduction in parasite burden in the spleen and a decrease in IL-10 and TGF-β production in L. infantum-infected BALB/c mice (29).…”

Section: Discussionmentioning

confidence: 99%

Integrated multivariate analysis of transcriptomic data reveals immunological mechanisms in mice afterLeishmania infantuminfection

Palacios

Diaz-Solano²,

Valladares³

et al. 2020

Preprint

View full text Add to dashboard Cite

Transcriptional analysis of complex biological scenarios has been extensively used, even though sometimes results may prove imprecise or difficult-to interpret due to an overwhelming amount of information. In this study, a large-scale Real-time qPCR experiment was coupled to multivariate statistical analysis to describe the main immunological events underlying the early L. infantum infection in livers of BALB/c mice. High-throughput qPCR was used to evaluate the expression of 223 genes related to immunometabolism 1-, 3-, 5- and 10-days post infection. This integrative analysis showed strikingly different gene signatures at 1- and 10-days post infection, revealing progression of infection in the experimental model based on the upregulation of particular immunological response patterns and mediators. This approach addresses the challenge of integrating large collections of transcriptional data for the identification of candidate biomarkers in experimental models.

show abstract

“…3 for qRT-PCR (i.e., E19, PN0, PN7, PN14, PN21, PN28, PN48, PN65). To test the efficacy of the primary antibody, we compared the molecular weight from a tissue sample known to have high levels of Foxp3 protein, the spleen, to the three brain regions we quantified (Eufrasio de Figueiredo et al, 2019). The observed molecular weight in the spleen was identical to the protein levels from the brain, (Fig.…”

Section: Foxp3 Protein Measurements By Western Blotmentioning

confidence: 99%

Age, but Not Sex, Modulates Foxp3 Expression in the Rat Brain across Development

et al. 2020

View full text Add to dashboard Cite

The interconnectivity between brain development and the immune system has become an area of interest for many neuroscientists. However, to date, a limited number of known immune mediators of the peripheral nervous system (PNS) have been found to influence the development of the central nervous system (CNS). FOXP3 is a well-established mediator of regulatory T-cells in the PNS. However, the expression pattern of FOXP3 in the CNS and the PNS throughout development is unknown. To fill this void, we have characterized, in several brain regions, the developmental profile of Foxp3 for both sexes using rats. We found different patterns of Foxp3 in the CNS and PNS. In the CNS, we found Foxp3 was ubiquitously expressed, with the levels of Foxp3 varying by brain region. We also found both Foxp3 mRNA and protein levels peak during embryonic development and then steadily decrease with a peak increase during adulthood. In adulthood, the protein but not mRNA increases to the equivalent levels found at the embryonic stage of life. In the PNS, Foxp3 protein levels were low embryonically and increased steadily over the life of the animal with maximal levels reached in adulthood. Patterns observed for both the PNS and CNS were similar in males and females across all developmental timepoints. Our novel findings have implications for understanding how the neural immune system impacts neurodevelopmental disorders such as autism and schizophrenia.

show abstract

CXCL10 treatment promotes reduction of IL-10+ regulatory T (Foxp3+ and Tr1) cells in the spleen of BALB/c mice infected by Leishmania infantum

Cited by 19 publications

References 27 publications

Cytokines and splenic remodelling during Leishmania donovani infection

Cytokines and splenic remodelling during Leishmania donovani infection

Integrated multivariate analysis of transcriptomic data reveals immunological mechanisms in mice afterLeishmania infantuminfection

Age, but Not Sex, Modulates Foxp3 Expression in the Rat Brain across Development

Contact Info

Product

Resources

About