CY5 fluorescence measured with silicon photomultipliers

Santangelo, Maria Francesca; Cono, S. La; Vasquez, Pedro; Fallica, Giorgio; Conoci, Sabrina; Busacca, Alessandro; Pagano, Roberto; Sciuto, Emanuele Luigi; Lombardo, S.; Libertino, Sebania

doi:10.1109/biocas.2014.6981718

Cited by 6 publications

(5 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Aim of our work was to define if the SiPMs used were able to detect the florescence signal of a common dye, we used the CY5, and if a different dye could be used as well. The experimental set-up we used, the components are described before, is schematically shown in Figure 1 [23]. We tested the emission signal as a function of both the detection angle and the fluorophore concentration in the sample.…”

Section: Optical Transduction Methodsmentioning

confidence: 99%

“…The set up for optical measurements [23], schematically reported in Figure 1, consisted of a light source provided by a Coherent Laser Cube at 660 nm impinging normally the sample, an optical filter (-30dB), a Keithley 236 source meter unit (SMU) to bias the SiPM and collect the signal through an electrical filter (when the measurement was performed in continuous mode), a goniometer, to change the detector/ sample angle (we used 60° mostly), a digital oscilloscope (Tektronix DPO7104, BW 1GHz) to detect the signal when the device operation was pulsed. A pulse generator (Agilent 81110A) was used to electrically modulate the excitation laser using a square waveform with period of 10 ms and width of 3.9 ns.…”

Section: Measurements Set Upmentioning

confidence: 99%

See 1 more Smart Citation

Optical and Electrical Si-Based Biosensors: Fabrication and Trasduction Issues

MF¹

2014

J Anal Bioanal Tech

View full text Add to dashboard Cite

In this work we discussed feasibility studies and examples of biological molecules integration in Si-based miniaturized devices. We investigated three main issues: (i) device surface functionalization, (ii) biological molecule functionality after immobilization and (iii) biosensor working principle using both electrical and optical transduction mechanisms. In the first case the idea is to fabricate electrolyte-insulator-semiconductor (EIS) and, in the near future, ion-sensitive field effect transistor (ISFET) biosensors. The electrical characterization of MOS-like capacitors with ssDNA anchored on the SiO 2 dielectric, allowed us to conclude that the structures tested are sensitive to DNA immobilization and hybridization, as demonstrated by a positive shift in the flat band voltage after ssDNA immobilization and by a further shift after prefect match hybridization. The optical transduction mechanism, using an approach closer to commercial devices (e.g. DNA-chip) is based on the use of pixelated solid state photon-detectors (Silicon Photomultipliers, SiPM). We showed this new device can be used to replace traditional optical detector in DNA-chip applications and allows designing new optical detection systems based on photon counting operation.

show abstract

Section: Optical Transduction Methodsmentioning

confidence: 99%

Section: Measurements Set Upmentioning

confidence: 99%

Optical and Electrical Si-Based Biosensors: Fabrication and Trasduction Issues

MF¹

2014

J Anal Bioanal Tech

View full text Add to dashboard Cite

show abstract

“…A SiPM can be integrated in a biosensor serving as the optical transducer and detecting small changes in absorbance, luminescence, polarization, or refractive index between reactants and products of a biological process [47,48]. Bio-luminescence and light scattering are optical phenomena used by biosensors that have proven most effective in the field of bioimaging research [49,50]; on the other hand, the collection of photoluminescence is very difficult due its extremely low intensity, typically in the order of a micro-lux [51,52]. Consequently, the challenge associated with collecting these optical signals with sufficient signal-to-noise ratio is enormous.…”

Section: Optical Biosensorsmentioning

confidence: 99%

Silicon Photomultiplier Current and Prospective Applications in Biological and Radiological Photonics

Stagliano

Chierici

Abegão

et al. 2018

EPHIJSE

View full text Add to dashboard Cite

The detection of low-intensity light is a crucial issue in many aspects of science and technology. So far, the solution has involved extremely delicate and somewhat bulky devices, the photomultiplier tubes, providing high sensitivity but suffering from fragility and susceptibility to interference. The silicon photomultiplier (SiPM) is a solid-state photon detector that provides a new solution for a wide range of photometry applications in fields as diverse as medicine, biology, environmental science, chemistry, physics, and nuclear physics. SiPMs are on a par with conventional photomultiplier tubes (PMT) in terms of internal gain and photon detection efficiency, while they are undoubtedly superior in terms of mechanical robustness, compact size, electronic stability, low power consumption, and affordability. Our group has long been involved in ionizing radiation measurements based on light emitting sensors both for industrial nuclear technology applications and for hybrid diagnostic imaging techniques. In both cases, SiPMs offer the unquestionable advantages described in this review.

show abstract

“…Silicon photomultipliers (SiPM) are significantly less expensive than PMTs but have similar gains (10 5–6 ) and have been suggested for use as fluorescence detectors for low-cost, CE instruments. , However, applications using SiPMs as a fluorescence detector in analytical instruments have only recently been explored. − SiPMs consist of arrays of micron-sized photodiodes (i.e., 50 × 50 μm dimensions) that are biased as single-photon avalanche diodes (SPAD) . Each microcell produces a digital current signal when struck by a photon, with the entire array producing an analog signal at higher light densities .…”

mentioning

confidence: 99%

Silicon Photomultipliers as a Low-Cost Fluorescence Detector for Capillary Electrophoresis

2020

View full text Add to dashboard Cite

Capillary electrophoresis (CE) is a highly efficient separation method capable of handling small sample volumes (~pL) and low (~yoctomole) detection limits, and as such is ideal for applications that require high sensitivity such as single-cell analysis. Low-cost CE instrumentation is quickly expanding but low-cost, open-source fluorescence detectors with ultra-sensitive detection limits are lacking. Silicon photomultipliers (SiPM) are inexpensive, low-footprint detectors with the potential to fill the role as a detector when cost, size, and customization are important. In this work we demonstrate the use of a SiPM in CE with zeptomolar detection limits and a dynamic range spanning five orders of magnitude, comparable to photomultiplier detectors. We characterize the performance of the SiPM as a highly sensitive detector by measuring enzyme activity in single cells. This simple, small footprint, and low-cost (<$130) light detection circuit will be beneficial for open-source, portable, and budget friendly instrumentation requiring high sensitivity. File list (2) download file view on ChemRxiv SiPM_CE_Detector.pdf (342.26 KiB) download file view on ChemRxiv Supplemental_Information.pdf (308.35 KiB)

show abstract

CY5 fluorescence measured with silicon photomultipliers

Cited by 6 publications

References 13 publications

Optical and Electrical Si-Based Biosensors: Fabrication and Trasduction Issues

Optical and Electrical Si-Based Biosensors: Fabrication and Trasduction Issues

Silicon Photomultiplier Current and Prospective Applications in Biological and Radiological Photonics

Silicon Photomultipliers as a Low-Cost Fluorescence Detector for Capillary Electrophoresis

Contact Info

Product

Resources

About