Cyanidin-3-glucoside attenuates angiotensin II-induced oxidative stress and inflammation in vascular endothelial cells

Sivasinprasasn, Sivanan; Pantan, Rungusa; Thummayot, Sarinthorn; Tocharus, Jiraporn; Suksamrarn, Apichart; Tocharus, Chainarong

doi:10.1016/j.cbi.2016.10.022

Cited by 45 publications

(27 citation statements)

References 42 publications

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“…In this study, we used Ang II to construct an EC oxidative stress model. As agreed with previous reports [27, 34], Ang II resulted in increasing of apoptosis, overproduction of ROS, and impairment of angiogenic function (tube formation ability and VEGF secretion), along with the upregulation of Nox2 level and downregulation of the ratio of p-VEGFR2/VEGFR2 in ECs.…”

Section: Discussionsupporting

confidence: 93%

NPC‐EXs Alleviate Endothelial Oxidative Stress and Dysfunction through the miR‐210 Downstream Nox2 and VEGFR2 Pathways

Liu

Wang

Chen

et al. 2017

Oxidative Medicine and Cellular Longevity

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We have demonstrated that neural progenitor cells (NPCs) protect endothelial cells (ECs) from oxidative stress. Since exosomes (EXs) can convey the benefit of parent cells through their carried microRNAs (miRs) and miR-210 is ubiquitously expressed with versatile functions, we investigated the role of miR-210 in the effects of NPC-EXs on oxidative stress and dysfunction in ECs. NPCs were transfected with control and miR-210 scramble/inhibitor/mimic to generate NPC-EXscon, NPC-EXssc, NPC-EXsanti-miR-210, and NPC-EXsmiR-210. The effects of various NPC-EXs on angiotensin II- (Ang II-) induced reactive oxygen species (ROS) overproduction, apoptosis, and dysfunction, as well as dysregulation of Nox2, ephrin A3, VEGF, and p-VEGFR2/VEGFR2 in ECs were evaluated. Results showed (1) Ang II-induced ROS overproduction, increase in apoptosis, and decrease in tube formation ability, accompanied with Nox2 upregulation and reduction of p-VEGFR2/VEGFR2 in ECs. (2) Compared to NPC-EXscon or NPC-EXssc, NPC-EXsanti-miR-210 were less whereas NPC-EXsmiR-210 were more effective on attenuating these detrimental effects induced by Ang II in ECs. (3) These effects of NPC-EXsanti-miR-210 and NPC-EXsmiR-210 were associated with the changes of miR-210, ephrin A3, VEGF, and p-VEGFR2/VEGFR2 ratio in ECs. Altogether, the protective effects of NPC-EXs on Ang II-induced endothelial injury through miR-210 which controls Nox2/ROS and VEGF/VEGFR2 signals were studied.

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Section: Discussionsupporting

confidence: 93%

NPC‐EXs Alleviate Endothelial Oxidative Stress and Dysfunction through the miR‐210 Downstream Nox2 and VEGFR2 Pathways

Liu

Wang

Chen

et al. 2017

Oxidative Medicine and Cellular Longevity

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“…C-3-Glu is one of the most extensively studied anthocyanins shown to possess various potential beneficial effects against oxidative stress and inflammation (50), insulin resistance (51), and dyslipidemia (52). Although C-3-Glu could be one of the potential bioactive compounds account for renal protective effect of lingonberry, future studies are warranted to evaluate the impact of other bioactive compounds in lingonberry.…”

Section: Discussionmentioning

confidence: 99%

Inhibition of Inflammatory Cytokine Expression Prevents High-Fat Diet-Induced Kidney Injury: Role of Lingonberry Supplementation

et al. 2020

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Chronic low-grade inflammation is a major stimulus for progression of chronic kidney disease (CKD) in individuals consuming high-fat diet. Currently, there are limited treatment options for CKD other than controlling the progression rate and its associated complications. Lingonberry (Vaccinium vitis-idaea L.) is rich in anthocyanins with demonstrated anti-inflammatory effect. In the current study, we investigated the potential renal protective effect of lingonberry and its anthocyanin (cyanidin-3-glucoside) in high-fat diet fed obese mice and in human proximal tubular cells. Prolonged consumption of high-fat diets is strongly associated with obesity, abnormal lipid and glucose metabolism. Mice (C57BL/6J) fed a high-fat diet (62% kcal fat) for 12 weeks developed renal injury as indicated by an elevation of blood urea nitrogen (BUN) level as well as an increase in renal kidney injury molecule-1 (KIM-1), neutrophil gelatinase-associated lipocalin (NGAL) and renin expression. Those mice displayed an activation of nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) and increased expression of inflammatory cytokines-monocyte chemoattractant-1 (MCP-1), tumor necrosis factor alpha (TNF-α), interleukin-6 (IL-6) in the kidneys. Mice fed a high-fat diet also had a significant elevation of inflammatory cytokine levels in the plasma. Dietary supplementation of lingonberry for 12 weeks not only attenuated high-fat diet-induced renal inflammatory response but also reduced kidney injury. Such a treatment improved plasma lipid and glucose profiles, reduced plasma inflammatory cytokine levels but did not affect body weight gain induced by high-fat diet feeding. Lingonberry extract or its active component cyanidin-3-glucoside effectively inhibited palmitic acid-induced NF-κB activation and inflammatory cytokine expression in proximal tubular cells. These results suggest that lingonberry supplementation can reduce inflammatory response and prevent chronic kidney injury. Such a renal protective effect by lingonberry and its active component may be mediated, in part, through NF-κB signaling pathway.

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“…These results suggest that ordinary and catalytic antioxidants may exhibit protective properties against, or suppress the morbidity of, DN, and a combination of methods to suppress excessive production of ROS, and thus increase the elimination of preformed ROS, may serve as a potential therapeutic agent against the development and progression of DN. C3G has been suggested to possess vigorous antioxidant and anti-inflammatory properties ( 23 , 24 ). Recent studies have revealed that C3G is able to suppress 1,3-dichloro-2-propanol-induced ROS generation and suppress the apoptosis of R2C leydig cells ( 25 ).…”

Section: Discussionmentioning

confidence: 99%

Anthocyanins inhibit high glucose-induced renal tubular cell apoptosis caused by oxidative stress in db/db mice

Wei

Zhang

et al. 2018

Int J Mol Med

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Oxidative stress is an important contributory factor resulting the development of kidney injury in patients with diabetes. Numerous in vitro and in vivo studies have suggested that anthocyanins, natural phenols commonly existing in numerous fruits and vegetables, exhibit important anti-oxidative, anti-inflammatory and antihyperlipidemic effects; however, their effects and underlying mechanisms on diabetic nephropathy (DN) have not yet been fully determined. In the present study, the regulation of apoptosis metabolism and antioxidative effects exhibited by anthocyanins [grape seed procyanidin (GSPE) and cyanidin-3-O-β-glucoside chloride (C3G)] were investigated, and the molecular mechanism underlying this process was investigated in vivo and in vitro. GSPE administration was revealed to suppress renal cell apoptosis, as well as suppress the expression of Bcl-2 in diabetic mouse kidneys. Furthermore, GSPE administration was demonstrated to suppress the expression of thioredoxin interacting protein (TXNIP), in addition to enhancing p38 mitogen-activation protein kinase (MAPK) and extracellular signal-regulated kinase 1/2 (ERK1/2) oxidase activity in diabetic mouse kidneys. In vitro experiments using HK-2 cells revealed that C3G suppressed the generation of HG-mediated reactive oxygen species, cellular apoptosis, the expression of cleaved caspase-3 and the Bax/Bcl-2 ratio; and enhanced the expression of cytochrome c released from mitochondria. Furthermore, treatment with C3G was revealed to suppress the expression of TXNIP, in addition to the phosphorylation of p38 MAPK and ERK1/2 oxidase activity in HK-2 cells under HG conditions. In addition, treatment with C3G was revealed to attenuate the HG-induced suppression of the biological activity of thioredoxin, and to enhance the expression of thioredoxin 2 in HK-2 cells under HG conditions. In conclusion, the present study demonstrated that anthocyanins may exhibit protective effects against HG-induced renal injury in DN via antioxidant activity.

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Cyanidin-3-glucoside attenuates angiotensin II-induced oxidative stress and inflammation in vascular endothelial cells

Cited by 45 publications

References 42 publications

NPC‐EXs Alleviate Endothelial Oxidative Stress and Dysfunction through the miR‐210 Downstream Nox2 and VEGFR2 Pathways

NPC‐EXs Alleviate Endothelial Oxidative Stress and Dysfunction through the miR‐210 Downstream Nox2 and VEGFR2 Pathways

Inhibition of Inflammatory Cytokine Expression Prevents High-Fat Diet-Induced Kidney Injury: Role of Lingonberry Supplementation

Anthocyanins inhibit high glucose-induced renal tubular cell apoptosis caused by oxidative stress in db/db mice

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