Cyanidin-3-glucoside from Black Rice Ameliorates Diabetic Nephropathy via Reducing Blood Glucose, Suppressing Oxidative Stress and Inflammation, and Regulating Transforming Growth Factor β1/Smad Expression

Zheng, Hong; Qi, Shan; He, Jia; Hu, Ching Yuan; Han, Ho Jae; Jiang, Hai; Li, Xin Shen

doi:10.1021/acs.jafc.0c00680

Cited by 66 publications

(46 citation statements)

References 47 publications

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“…SOD reduces superoxide radicals to hydrogen peroxide by spontaneous dismutation, while GSH-Px and CAT reduces excess hydrogen peroxide to water, thus regulating ROS level in the body. [28,29] Interestingly, NRCE abrogated the oxidative stress-mediated CISP toxicity, and alleviated the impact of CISP on the testis histology. It was observed that the level of NRCE abrogation was consistent with the doses (50, 100 and 400 mg/kg) used.…”

Section: Discussionmentioning

confidence: 94%

“…The physiological functions of these antioxidant enzymes are interconnected. SOD reduces superoxide radicals to hydrogen peroxide by spontaneous dismutation, while GSH‐Px and CAT reduces excess hydrogen peroxide to water, thus regulating ROS level in the body [28,29] . Interestingly, NRCE abrogated the oxidative stress‐mediated CISP toxicity, and alleviated the impact of CISP on the testis histology.…”

Section: Discussionmentioning

confidence: 98%

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Protective Effects of Nucleosides‐Rich Extract from Cordyceps cicadae against Cisplatin Induced Testicular Damage.

Pei

et al. 2020

Chemistry & Biodiversity

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Cisplatin (CISP) is an efficacious anticancer agent used in chemotherapy, however, the constraint to its clinical utility is the stray organ toxicity including testicular damage linked to oxidative and inflammatory cascades. This study aimed to explore the protective effect of nucleosides-rich extract from Cordyceps cicadae (NRCE) against CISP-induced testicular damage in rats. Rats were subjected to prophylactic oral administration of NRCE (50, 100 and 400 mg/kg body weight/day) for 7 days prior to testicular toxicity induced by CISP (10 mg/kg, ip) and were sacrificed after 72 h post-CISP injection. Cisplatin caused significant deficits in sperm count, viability and motility, testosterone and follicle stimulating hormone (FSH) compared to normal control. It depressed testicular activities of glutathione peroxidase (GSH-Px), superoxide dismutase (SOD), catalase (CAT), total antioxidant content (TAC), whereas malondialdehyde (MDA) increased remarkably. CISP considerably increased tumor necrosis factor-alpha (TNF-α) and interleukin-one beta (IL-1β) with alterations in testis histology compared to normal control. Interestingly, NRCE pretreatment inhibited the CISP-induced alterations in reproductive indices, restored the antioxidant activities in testes as well as inflammatory mediators and histology comparable to control. Our findings demonstrate that NRCE could prevent CISP testicular damage via inhibition of oxidative stress and proinflammation in rats.

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Section: Discussionmentioning

confidence: 94%

Section: Discussionmentioning

confidence: 98%

Protective Effects of Nucleosides‐Rich Extract from Cordyceps cicadae against Cisplatin Induced Testicular Damage.

Pei

et al. 2020

Chemistry & Biodiversity

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“…43 Another review elucidates that the activation of NF-κB can aggravate renal fibrosis by promoting the expression of Col-IV and FN. 44 Therefore, the mechanism by which wogonin affects renal inflammation and fibrosis by regulating these molecules is an open question.…”

Section: Discussionmentioning

confidence: 99%

<p>Wogonin Ameliorates Renal Inflammation and Fibrosis by Inhibiting NF-κB and TGF-β1/Smad3 Signaling Pathways in Diabetic Nephropathy</p>

Zheng¹,

Zhu²,

Lei³

et al. 2020

DDDT

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Introduction: Diabetic nephropathy (DN) has become an increasing threat to health, and inflammation and fibrosis play important roles in its progression. Wogonin, a flavonoid, has been proven to suppress inflammation and fibrosis in various diseases, including acute kidney injury. This study aimed at investigating the effect of wogonin on diabetes-induced renal inflammation and fibrosis. Materials and Methods: Streptozotocin (STZ)-induced diabetic mouse models received gavage doses of wogonin (10, 20, and 40 mg/kg) for 12 weeks. Metabolic indices from blood and urine and pathological damage of glomerulus in the diabetic model were assessed. Glomerular mesangial cells SV40 were cultured in high glucose (HG) medium containing wogonin at concentrations of 1.5825, 3.125, and 6.25 μg/mL for 24 h. Inflammation and fibrosis indices were evaluated by histopathological, Western blotting, and PCR analyses. Results: Wogonin treatment ameliorated albuminuria and histopathological lesions in diabetic mice. Inflammatory cytokines, such as monocyte chemotactic protein-1 (MCP-1), tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), and related signaling pathway NF-κB were downregulated after the administration of wogonin in vivo and in vitro. Furthermore, wogonin reduced the expression of extracellular matrix (ECM), including fibronectin (FN), collagen IV (Col-IV), α-smooth muscle actin (α-SMA), and transforming growth factor-β1 (TGF-β1) in the kidneys of diabetic mice and HG-induced mesangial cells. Moreover, the inhibition of TGF-β1/Smad3 pathway might be responsible for these changes. Conclusion: Wogonin may ameliorate renal inflammation and fibrosis in diabetic nephropathy by inhibiting the NF-κB and TGF-β1/Smad3 signaling pathways.

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“…The activation of the Smad pathway and its subsequent nuclear transposition are key steps in TGF-β1-mediated renal fibrosis in DN [13]. The phosphorylation of Smad2 and Smad3 is also an important signal transduction process in the TGF-β1/Smads signaling pathway, and their expression indicates TGF-β1/Smads signaling pathway activation [14]. The expression of TGF-β1, Smad2, p-Smad2, Smad3, p-Smad3, and α-SMA in mouse renal tissues was measured via western blotting.…”

Section: Dmix Inhibited the Tgf-β1/smads Signaling Pathway In The Renmentioning

confidence: 99%

Dendrobium Mixture Improved Diabetic Nephropathy in db/db Mice by Regulating TGF-β1/Smads Signal Transduction

Chen

Lin

Zheng

et al. 2021

Preprint

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BackgroundDendrobium mixture (DMix) is an effective treatment for diabetic nephropathy (DN), but the underlying molecular mechanism remains unclear. In this study, we investigated whether DMix regulates the transforming growth factor-β1 (TGF-β1)/Smads signal transduction pathway. MethodsTwenty-four db/db mice were randomly divided into three groups: the model, DMix, and gliquidone groups, while eight db/m mice were selected as the normal control group. The drug was administered by continuous gavage for 8 weeks. Body weight (BW), kidney weight (KW), kidney index, fasting blood glucose (FBG), blood lipid, 24-hour urinary albumin excretion rate, blood urea nitrogen, and serum creatinine levels were measured. Pathological changes in the renal tissue were observed using a light microscope. Real-time quantitative PCR and immunohistochemical staining were used to detect mRNA expression of TGF-β1 and alpha-smooth muscle actin (α-SMA) genes and proteins, respectively, in renal tissues. TGF-β1, Smad2, p-Smad2, Smad3, p-Smad3, and α-SMA expression levels were measured using western blotting. ResultsDMix significantly reduced FBG level, BW, KW, and blood lipid level, and improved renal function in db/db mice. Histopathology showed that DMix alleviated glomerular mesangial cell proliferation and renal interstitial fibrosis in db/db mice. Additionally, DMix reduced protein and mRNA expression of TGF-β1 and α-SMA, and inhibited Smad2 and Smad3 phosphorylation. ConclusionsThe findings suggest that DMix may inhibit renal fibrosis and delay the progression of DN by regulating the TGF-β1/Smads signaling pathway. Key words: Diabetic nephropathy, Dendrobium mixture, TGF-β1/Smads signaling pathway

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Cyanidin-3-glucoside from Black Rice Ameliorates Diabetic Nephropathy via Reducing Blood Glucose, Suppressing Oxidative Stress and Inflammation, and Regulating Transforming Growth Factor β1/Smad Expression

Cited by 66 publications

References 47 publications

Protective Effects of Nucleosides‐Rich Extract from Cordyceps cicadae against Cisplatin Induced Testicular Damage.

Protective Effects of Nucleosides‐Rich Extract from Cordyceps cicadae against Cisplatin Induced Testicular Damage.

<p>Wogonin Ameliorates Renal Inflammation and Fibrosis by Inhibiting NF-κB and TGF-β1/Smad3 Signaling Pathways in Diabetic Nephropathy</p>

Dendrobium Mixture Improved Diabetic Nephropathy in db/db Mice by Regulating TGF-β1/Smads Signal Transduction

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