Cyanidin-3-glucoside isolated from mulberry fruits protects pancreatic β-cells against glucotoxicity-induced apoptosis

Abstract

The present study investigated the cytoprotective effects of cyanidin‑3‑glucoside (C3G), isolated from mulberry fruits, on the glucotoxicity‑induced apoptosis of pancreatic β‑cells to evaluate the antidiabetic effects of this compound. MIN6N pancreatic β‑cells were used to investigate the cytoprotective effects of C3G. In addition, the effects of C3G on the glucotoxicity‑induced apoptosis of pancreatic β‑cells was evaluated using MTT assay, immunofluorescent staining, flow cytometric and western blot analyses. The pancreatic β‑cells cultured under high glucose conditions exhibited distinct apoptotic features. C3G decreased the generation of intracellular reactive oxygen species, DNA fragmentation and the rate of apoptosis. C3G also prevented pancreatic β‑cell apoptosis induced by high glucose conditions by interfering with the intrinsic apoptotic pathways. In addition, C3G treatment resulted in increased insulin secretion compared with treatment with high glucose only. In conclusion, the results of the present study suggested that C3G obtained from mulberry fruits may be a potential phytotherapeutic agent for the prevention of diabetes.

Mulberry Anthocyanin Extract Ameliorates Oxidative Damage in HepG2 Cells and Prolongs the Lifespan of Caenorhabditis elegans through MAPK and Nrf2 Pathways

“…Studies have shown that cyanidin-3-glucoside isolated from mulberry fruit restrains high-glucose/H 2 O 2 -induced pancreatic β -cell death [30, 31]. In concurrence with previous studies, decreases in glucose consumption and uptake were observed in H 2 O 2 -induced HepG2 cells, meaning insulin resistance was formed.…”

Comparison of anticancer activity and antioxidant activity between cyanidin-3-O-glucoside liposomes and cyanidin-3-O-glucoside in Caco-2 cells in vitro

“…1,[5][6][7][8][9] Several studies reported that C3G protects against the adverse effects of UV-B radiation, controls the key aspects of tumorigenesis, inhibits the proliferation and induces the apoptosis of cancer cells, reduces oxidative stress, ghts H 2 O 2induced oxidative stress in human embryonic kidney (HEK-293) cells, and induces cell apoptosis and inhibits cell migration in TNF-a-challenged RASMCs in vitro. [10][11][12][13][14][15][16] Fratantonio found that C3G pretreatment effectively reverses the effects of PA on the PI3K/Akt axis and restores the eNOS expression and NO release. 17 Sivasinprasasn revealed that pretreatment with C3G inhibits the NF-kB signaling pathway.…”

Cyanidin Protects SH-SY5Y Human Neuroblastoma Cells from 1-Methyl-4-Phenylpyridinium-Induced Neurotoxicity

“…Cyanidin is demonstrated to be beneficial against diabetes [4,5], cancer [6][7][8], and atherosclerosis [9]. In the central nervous system, recent investigations indicate that cyanidin showed potential protections against Alzheimer's disease and multiple sclerosis [10,11].…”