Cyanidin-3-glucoside suppresses the progression of lung adenocarcinoma by downregulating TP53I3 and inhibiting PI3K/AKT/mTOR pathway

Abstract

Background The aim of this study is to unravel the role of Cyanidin-3-glucoside (C3G) and its potential mechanisms in lung adenocarcinoma (LUAD). Methods The cell clones, proliferation, apoptosis, migration, and invasion in H1299 and A549 cells were determined by colony formation assay, 5-ethynyl-20 deoxyuridine (EdU) assay, flow cytometry, and transwell assay, respectively. The expression of p53-induced gene 3 (TP53I3) was assessed and the prognostic values of TP53I3 in LUAD via the dataset from the Cancer Genome Atlas (TCGA). In addition, the mRNA and protein expressions were detected by quantitative real-time PCR (qRT-PCR) and western blot. Results C3G inhibited the proliferation, migration, and invasion of, and also promoted the apoptosis in H1299 and A549 cells. The database of TCGA showed TP53I3 was highly expressed in LUAD tissues and correlated with the poor prognosis of LUAD patients. Moreover, we also found that C3G inhibited the proliferation, migration and invasion, and promoted apoptosis in H1299 and A549 cells by downregulating TP53I3. Additionally, C3G could inhibit the activation of phosphatidylinositol 3′-kinase (PI3K)/protein kinase B (AKT)/mammalian target of rapamycin (mTOR) pathway in H1299 and A549 cells by downregulating TP53I3. Conclusion This study demonstrated that C3G could inhibit the proliferation, migration and invasion, and also facilitate the apoptosis through downregulating TP53I3 and inhibiting PI3K/AKT/mTOR pathway in LUAD.

Behind the Scenes of Anthocyanins—From the Health Benefits to Potential Applications in Food, Pharmaceutical and Cosmetic Fields

“…Lu et al [ 158 ], for instance, showed that the ACNs extracted from the fruits of Vitis coignetiae Pulliat, widely used in Korean folk medicine to treat inflammatory diseases and cancers, inhibit the expression of several transcription and growth factors that promote proliferation, angiogenesis, invasion, and migration of non-small cell LC (NSCLC) cells. Similar results were obtained when LC model cells were assayed with Cy3glu and Dp solutions, unveiling the downregulation of specific pathways [ 159 ] and transcription factors [ 160 ].…”

Cyanidin as potential anticancer agent targeting various proliferative pathways

“…Besides these C3G also facilitates apoptosis by inhibiting the activation of the mammalian target of rapamycin (mTOR) as well as the protein kinase B (AKT) pathway and suppression of TP53I3 in LC cells. This data suggest that C3G is an effective compound for treating LC (Chen et al, 2021).…”

Pan-cancer landscape of CENPO and its underlying mechanism in LUAD

“…Previous studies had shown that activation of mTOR promotes tumor growth and metastasis [ 17 ]. The PIK3CA mutation is also a common feature of LUAD, which is related to a poor prognosis [ 18 ]. Alterations in the cell cycle and PI3K pathways were associated with recurrence of LUAD [ 19 ].…”