Cyanobacteria-based microbial cell factories for production of industrial products

Hamouda, Ragaa A.; El‐Naggar, Noura El‐Ahmady

doi:10.1016/b978-0-12-821477-0.00007-6

Cited by 11 publications

(8 citation statements)

References 280 publications

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“…Not to mention, Indonesia, as an archipelagic country, is rich in marine resources. As the major pigment compound in Spirulina, it is responsible for around 50% of light uptake in photosynthesis (Mishra et al, 2012;Queiroz et al, 2020) and has been shown to have reactive capacity against oxygen species, such as hydroxyl radical, alkoxyl radical, and peroxyl radical, that presents anti-inflammatory (Tripathi et al, 2021), neuroprotective, andhepatoprotective (Fernández-Rojas et al, 2014;Hamouda and El-Naggar, 2021) properties. Previous studies showed that phycocyanin was also valuable in managing diverse types of cancers.…”

Section: Molecular Dynamics Simulation Assessmentmentioning

confidence: 99%

Computational simulations of microalgae-derived bioactive compounds as a novel inhibitor against B-Raf V600E-driven melanoma

Prasetiya¹,

Destiarani²,

Prihastaningtyas³

et al. 2023

J App Pharm Sc

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Melanoma is one of the most aggressive types of cancer, which has shown a tremendous surge in the last 50 years. Therapy for advanced-type melanoma is still a challenge because of the low response rate and 10-year survival. Therefore, drug discovery efforts need to be made to fight this cancer. To date, the development of big data and 3D has made it easier for researchers to understand the structure of proteins or enzymes that play an important role as receptors in melanoma cancer to be used as specific targets for diagnosis and therapy, for instance, the B-Raf V600E. This study examined the potential of active compounds from microalgae for developing melanoma anticancer drugs. The database was constructed using data mining from MarinLit and the related publications from 1970 to 2020. In silico methods such as molecular docking, virtual screening, and molecular dynamic simulations were used to find the most potential candidates. A total of 25 compounds passed the virtual screening stage. The top three compounds based on the binding free energy compared to a natural ligand and commercial drug are cholesta-5,7-dien-3beta-ol, 24-oxocholesterol acetate, lathosterol, and two additional compounds, phycocyanin and phycocyanobilin, were also selected due to their massive production from the most commonly cultured microalgae worldwide, Arthrospira sp. (previously known as Spirulina sp.). Furthermore, ADME analysis and toxicity tests were also carried out. Molecular dynamics simulation showed that phycocyanin was the best potential candidate for melanoma anticancer drugs, with free binding energies ranging from −65 to −80 kcal/mol. This result was also supported by root mean square deviation, root mean square fluctuation, and distance parameter data. This study may accelerate molecular research in producing therapeutic compounds for melanoma cancer, thus allowing it to continue developing pharmaceutical products that benefit human health.

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Section: Molecular Dynamics Simulation Assessmentmentioning

confidence: 99%

Computational simulations of microalgae-derived bioactive compounds as a novel inhibitor against B-Raf V600E-driven melanoma

Prasetiya¹,

Destiarani²,

Prihastaningtyas³

et al. 2023

J App Pharm Sc

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“…The COD removal efficiency using devilfish bone char bioanode was slightly higher (1.09 times) than carbon felt. The reduction in COD suggests that the bacteria used pantoprazole as a carbon source and produced bioenergy from its biochemical reactions (Hamouda and El-Naggar, 2021).…”

Section: Cod Removal E Ciencymentioning

confidence: 99%

“…Microbial fuel cells (MFCs) are bioelectrochemical processes that aim to produce bioenergy (electricity) by using the electrons derived from biochemical reactions catalyzed by bacteria (Hamouda and El-Naggar, 2021). Generally, the MFC contains an anode and a cathode, separated by a proton exchange membrane.…”

Section: Introductionmentioning

confidence: 99%

Devilfish bone char, an alternative material to be used as bioanode in microbial fuel cells

Flores

Vázquez²,

Castillo³

2022

Front. Sustain.

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This work aimed to evaluate the devilfish bone char as an alternative material to be used as a bioanode in a microbial fuel cell (MFC) applied in bioenergy production from the pantoprazole biodegradation. The devilfish is an invasive species in Mexico and other countries, so its control and eradication are based on the integral use of its biomass. In addition, the bones are unusable waste and could be used as a raw material to manufacture the bioanode. Two MFCs with an anaerobic anode single chamber of 125 mL were operated independently using the fabricated bioanode and the carbon felt (conventional anode). Both MFCs were fed with 1 g/L of pantoprazole as substrate with a chemical oxygen demand (COD) of 210 ± 1.3 mg/L and 1.2 g of soil as inoculum. The two MFCs were characterized electrochemically, and the COD removal percentage was measured as an indicator of the pantoprazole biodegradation. The results showed that the MFC operated with the bioanode had a better performance than the conventional anode, since it reached a maximum power density of 1.22 mW/m2, being 156% higher than carbon felt. These values were consistent with the COD removal percentages reached in 10 days with values of 47.17 ± 0.23 and 43.34 ± 0.41%, respectively. Therefore, these findings provide a low-cost alternative carbonaceous material for the sustainable construction of MFC applied to produce bioenergy from the biodegradation of pharmaceutical products.

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“…Third, cyanobacteria maintain a relatively low percentage of carbohydrates and a high level of proteins (10–30% carbohydrate, 40–79% protein) as compared to plants (70–80% carbohydrate, 1–10% protein). , This attribute makes cyanobacteria an ideal chassis for flavonoid biosynthesis, using AAAs as substrates. Finally, based on endosymbiotic theory, unicellular cyanobacteria are ancestors of modern chloroplasts, , and the composition of their cell wall and membrane do not exert any meaningful pathologic immune response when used in pharmaceutical applications. , Consequently, cyanobacteria can be quickly developed as a “green” chassis for the synthesis of various high-value bioproducts. − …”

Section: Benefits Of Photosynthetic Cyanobacteria As a Sustainable Ch...mentioning

confidence: 99%

Potential of Producing Flavonoids Using Cyanobacteria As a Sustainable Chassis

Jin

Wang

Zhao

et al. 2021

J. Agric. Food Chem.

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Numerous plant secondary metabolites have remarkable impacts on both food supplements and pharmaceuticals for human health improvement. However, higher plants can only generate small amounts of these chemicals with specific temporal and spatial arrangements, which are unable to satisfy the expanding market demands. Cyanobacteria can directly utilize CO 2 , light energy, and inorganic nutrients to synthesize versatile plant-specific photosynthetic intermediates and organic compounds in largescale photobioreactors with outstanding economic merit. Thus, they have been rapidly developed as a "green" chassis for the synthesis of bioproducts. Flavonoids, chemical compounds based on aromatic amino acids, are considered to be indispensable components in a variety of nutraceutical, pharmaceutical, and cosmetic applications. In contrast to heterotrophic metabolic engineering pioneers, such as yeast and Escherichia coli, information about the biosynthesis flavonoids and their derivatives is less comprehensive than that of their photosynthetic counterparts. Here, we review both benefits and challenges to promote cyanobacterial cell factories for flavonoid biosynthesis. With increasing concerns about global environmental issues and food security, we are confident that energy self-supporting cyanobacteria will attract increasing attention for the generation of different kinds of bioproducts. We hope that the work presented here will serve as an index and encourage more scientists to join in the relevant research area.

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Cyanobacteria-based microbial cell factories for production of industrial products

Cited by 11 publications

References 280 publications

Computational simulations of microalgae-derived bioactive compounds as a novel inhibitor against B-Raf V600E-driven melanoma

Computational simulations of microalgae-derived bioactive compounds as a novel inhibitor against B-Raf V600E-driven melanoma

Devilfish bone char, an alternative material to be used as bioanode in microbial fuel cells

Potential of Producing Flavonoids Using Cyanobacteria As a Sustainable Chassis

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