Cyanobacterial biorefinery: Towards economic feasibility through the maximum valorization of biomass

Prabha, Syama; Vijay, Aravind K.; Paul, Rony Rajan; George, Basil

doi:10.1016/j.scitotenv.2021.152795

Cited by 32 publications

(12 citation statements)

References 186 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Hence, proper choice of harvesting methodology is crucial while aiming for biomass harvest under a scaled-up system. Among the different techniques, pH-induced flocculation using NaOH has been reported as the most economical and eco-friendly harvesting method for large-scale set-ups 33 , 34 . Assuming a scaled-up cultivation system of 10,000 L employing the APL medium, the harvest cost is estimated to be 0.765 $.…”

Section: Resultsmentioning

confidence: 99%

A waste-to-wealth initiative exploiting the potential of Anabaena variabilis for designing an integrated biorefinery

Deb

Mallick

Bhadoria

2022

Sci Rep

View full text Add to dashboard Cite

The current research work was an innovative approach providing dual advantages of waste bioremediation and an effective biorefinery. The study attempted to exploit wastewater like aqua discharge and solid wastes like poultry litter/cow dung for cyanobacterial cultivation. Aqua discharge appended with 7.5 g L−1 poultry litter turned out as the best combination generating 46% higher carbohydrate yield than BG-11 control. A. variabilis cultivation in this waste-utilized medium also revealed its excellent bioremediation ability. While 100% removal was observed for nitrite, nitrate, and orthophosphate, a respective 74% and 81% reduction was noted for ammonium and total organic carbon. Chemical and biological oxygen demands were also reduced by 90%. This work was also novel in developing a sequential design for the production of bioethanol and co-products like exopolysaccharides, sodium copper chlorophyllin, C-phycocyanin, and poly-β-hydroxybutyrate from the same cyanobacterial biomass. The developed biorefinery implementing the waste-utilized medium was one of its kind, enabling biomass valorization of 61%. Therefore, the present study would provide a leading-edge for tackling the high production costs that limit the practical viability of biorefinery projects. The recyclability of the bioremediated wastewater would not only curtail freshwater usage, the waste disposal concerns would also be mitigated to a great extent.

show abstract

Section: Resultsmentioning

confidence: 99%

A waste-to-wealth initiative exploiting the potential of Anabaena variabilis for designing an integrated biorefinery

Deb

Mallick

Bhadoria

2022

Sci Rep

View full text Add to dashboard Cite

show abstract

“…Spirulina ( Artrhospira platensis ) belongs to the phylum Cyanobacteria , the family of Oscillatoriaceae that are known as blue-green algae [ 1 ]; it also contains proteins (phycocyanin), B-group vitamins, natural pigments (chlorophyll and carotenoids) and essential fatty acids. Spirulina have been widely recognized as a food and dietary supplement since the 16th century [ 2 , 3 ]. Recent research studies prove that spirulina has numerous health benefits such as antioxidant [ 4 ], immunomodulatory [ 5 ] and anti-inflammatory activities [ 6 ].…”

Section: Introductionmentioning

confidence: 99%

The Physicochemical Properties and Antioxidant Activity of Spirulina (Artrhospira platensis) Chlorophylls Microencapsulated in Different Ratios of Gum Arabic and Whey Protein Isolate

Zhang

et al. 2022

Foods

View full text Add to dashboard Cite

Spirulina (Artrhospira platensis) is rich in chlorophylls (CH) and is used as a potential natural additive in the food industry. In this study, the CH content was extracted from spirulina powder after ultrasound treatment. Microcapsules were then prepared at different ratios of gum Arabic (GA) and whey protein isolate (WPI) through freeze-drying to improve the chemical stability of CH. As a result, a* and C* values of the microcapsules prepared from GA:WPI ratios (3:7) were −8.94 ± 0.05 and 15.44 ± 0.08, respectively. The GA fraction increased from 1 to 9, and encapsulation efficiency (EE) of microcapsules also increased by 9.62%. Moreover, the absorption peaks of CH at 2927 and 1626 cm−1 in microcapsules emerged as a redshift detected by FT-IR. From SEM images, the morphology of microcapsules changed from broken glassy to irregular porous flake-like structures when the GA ratio increased. In addition, the coated microcapsules (GA:WPI = 3:7) showed the highest DPPH free radical scavenging activity (SADPPH) (56.38 ± 0.19) due to low moisture content and better chemical stability through thermogravimetric analysis (TGA). Conclusively, GA and WPI coacervates as the wall material may improve the stability of CH extracted from spirulina.

show abstract

“…Microalgae and cyanobacteria are promising candidates for omega-3 FA production due to their ability to convert atmospheric carbon dioxide into FAs [ 10 ]. Moreover, the culture of these photosynthetic organisms does not compete with arable land use [ 20 ] and can use wastewater as a culture medium [ 21 ]. A series of advantages make cyanobacteria a preferred organism to produce FAs rather than eukaryotic microalgae, including a simpler genetic background that eases manipulation and the availability of genetic tools that allow cyanobacterial genetic engineering [ 20 ].…”

Section: Introductionmentioning

confidence: 99%

“…Thus, different approaches have been used to boost the production of omega-3 FAs in cyanobacteria. The main strategy consists of the overexpression of endogenous and exogenous desaturases and enzymes involved in the introduction of a double bond in a specific position of long-chain FAs ( Figure 1 ) [ 21 , 25 , 26 , 27 , 28 ]. Modification of culture conditions, such as increasing the light intensity or/and decreasing the temperature during cyanobacterial culture, have been successfully used to improve omega-3 FA yields [ 29 , 30 , 31 , 32 ].…”

Section: Introductionmentioning

confidence: 99%

Synechococcus elongatus PCC 7942 as a Platform for Bioproduction of Omega-3 Fatty Acids

Santos-Merino

Gutiérrez-Lanza

Nogales

et al. 2022

Life

View full text Add to dashboard Cite

Alpha-linolenic acid and stearidonic acid are precursors of omega-3 polyunsaturated fatty acids, essential nutrients in the human diet. The ability of cyanobacteria to directly convert atmospheric carbon dioxide into bio-based compounds makes them promising microbial chassis to sustainably produce omega-3 fatty acids. However, their potential in this area remains unexploited, mainly due to important gaps in our knowledge of fatty acid synthesis pathways. To gain insight into the cyanobacterial fatty acid biosynthesis pathways, we analyzed two enzymes involved in the elongation cycle, FabG and FabZ, in Synechococcus elongatus PCC 7942. Overexpression of these two enzymes led to an increase in C18 fatty acids, key intermediates in omega-3 fatty acid production. Nevertheless, coexpression of these enzymes with desaturases DesA and DesB from Synechococcus sp. PCC 7002 did not improve alpha-linolenic acid production, possibly due to their limited role in fatty acid synthesis. In any case, efficient production of stearidonic acid was not achieved by cloning DesD from Synechocystis sp. PCC 6803 in combination with the aforementioned DesA and DesB, reaching maximum production at 48 h post induction. According to current knowledge, this is the first report demonstrating that S. elongatus PCC 7942 can be used as an autotrophic chassis to produce stearidonic acid.

show abstract

Cyanobacterial biorefinery: Towards economic feasibility through the maximum valorization of biomass

Cited by 32 publications

References 186 publications

A waste-to-wealth initiative exploiting the potential of Anabaena variabilis for designing an integrated biorefinery

A waste-to-wealth initiative exploiting the potential of Anabaena variabilis for designing an integrated biorefinery

The Physicochemical Properties and Antioxidant Activity of Spirulina (Artrhospira platensis) Chlorophylls Microencapsulated in Different Ratios of Gum Arabic and Whey Protein Isolate

Synechococcus elongatus PCC 7942 as a Platform for Bioproduction of Omega-3 Fatty Acids

Contact Info

Product

Resources

About