Cyanobacterial cytoskeleton immunostaining: the detection of cyanobacterial cell lysis induced by planktopeptin BL1125

Sedmak, Bojan; Carmeli, Shmuel; Pompe-Novak, Maruša; Tušek‐Žnidarič, Magda; Grach‐Pogrebinsky, Olga; Eleršek, Tina; Žužek, Monika C.; Bubik, Anja; Frangež, Robert

doi:10.1093/plankt/fbp076

Cited by 13 publications

(14 citation statements)

References 32 publications

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“…Cells were prepared and immunostained as reported [13]. The target in M. aeruginosa strains was the Unnamed protein product (accession number CAO86402) [14].…”

Section: Methodsmentioning

confidence: 99%

UVB Radiation as a Potential Selective Factor Favoring Microcystin Producing Bloom Forming Cyanobacteria

2013

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Due to the stratospheric ozone depletion, several organisms will become exposed to increased biologically active UVB (280–320 nm) radiation, not only at polar but also at temperate and tropical latitudes. Bloom forming cyanobacteria are exposed to UVB radiation on a mass scale, particularly during the surface bloom and scum formation that can persist for long periods of time. All buoyant species of cyanobacteria are at least periodically exposed to higher irradiation during their vertical migration to the surface that usually occurs several times a day. The aim of this study is to assess the influence on cyanobacteria of UVB radiation at realistic environmental intensities. The effects of two UVB intensities of 0.5 and 0.99 W/m2 in up to 0.5 cm water depth were studied in vitro on Microcystis aeruginosa strains, two microcystin producing and one non-producing. After UVB exposure their ability to proliferate was estimated by cell counting, while cell fitness and integrity were evaluated using light microscopy, autofluorescence and immunofluorescence. Gene damage was assessed by TUNEL assay and SYBR Green staining of the nucleoide area. We conclude that UVB exposure causes damage to the genetic material, cytoskeletal elements, higher sedimentation rates and consequent cell death. In contrast to microcystin producers (PCC7806 and FACHB905), the microcystin non-producing strain PCC7005 is more susceptible to the deleterious effects of radiation, with weak recovery ability. The ecological relevance of the results is discussed using data from eleven years’ continuous UVB radiation measurements within the area of Ljubljana city (Slovenia, Central Europe). Our results suggest that increased solar radiation in temperate latitudes can have its strongest effect during cyanobacterial bloom formation in spring and early summer. UVB radiation in this period may significantly influence strain composition of cyanobacterial blooms in favor of microcystin producers.

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“…Cells were prepared and immunostained as reported [13]. The target in M. aeruginosa strains was the Unnamed protein product (accession number CAO86402) [14].…”

Section: Methodsmentioning

confidence: 99%

UVB Radiation as a Potential Selective Factor Favoring Microcystin Producing Bloom Forming Cyanobacteria

2013

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“…Since different microcystin variants can sometimes induce quantitatively different responses in phototrophic organisms , the use of such extract may better simulate the situation in the environment, where cyanobacteria are exposed to the mixture of different microcystin variants and also to other metabolites of cyanobacterial origin. Although the recorded HPLC chromatograms show prominent microcystin peaks and there is a lack of any other significant non-microcystin peaks at wavelengths 220 and 280 nm, which are the absorption maxima of some other cyanobacterial oligopeptides like anabaenopeptins or cyanopeptolines (Sedmak et al 2009), we cannot rule out that such chemicals coproduced and co-eluted with microcystins might be also present in the extract and simply not being detected by our HPLC method. Therefore, these chemicals could have also contributed to or been responsible for modulation of cellular differentiation observed in our study.…”

Section: Discussionmentioning

confidence: 83%

“…Planktopeptin BL1125, anabaenopeptin B, and anabaenopeptin F were shown to alter growth and activate lytic cycle in M. aeruginosa (Sedmak et al , 2009. It is evident from these studies that natural function and ecological role of microcystin should not be studied separately from the other non-ribosomally synthesized cyanobacterial oligopeptides, which are probably evolutionary and functionally closely related to microcystins (Rantala et al 2004).…”

Section: Discussionmentioning

confidence: 99%

Extract of Microcystis water bloom affects cellular differentiation in filamentous cyanobacterium Trichormus variabilis (Nostocales, Cyanobacteria)

et al. 2010

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Cyanobacteria produce a wide spectrum of biologically active compounds such as microcystins, whose effects on photoautotrophic organisms and role in the aquatic ecosystems have been most intensively discussed and studied. In our study, we examined effects of semipurified Microcystis extract containing microcystins (0.2-20 nM corresponding to 0.2-20 μg L −1 ) on age-induced cell differentiation of filamentous cyanobacterium Trichormus variabilis. The heterocyst and akinete formation was significantly decreased after exposure to extract containing 2 or 20 nM of microcystins within 10 days of exposure. To our knowledge, this is the first information about effects of metabolites produced by planktonic cyanobacteria on cell differentiation in filamentous cyanobacteria. Since the effects were induced by environmentally relevant concentrations of microcystins, our observations may indicate not only that microcystins or other bioactive peptides could affect conservation, overwintering, and nitrogen-fixation in filamentous cyanobacteria under environmental conditions but also contribute to the understanding of possible signaling function of cyanobacterial metabolites.

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“…Plectonema boryanum, 10. Control; One population t test: t = 22.19803, P = 3.61309E-9, at 0.05 level, the two means are significantly different produced and contained within or confined to the interior of the cells and are released in the environment (Sedmak et al 2009). It is established that inoculated PGPRs release various kinds of secondary metabolites as growth promoting substances (Khalid et al 2006;Ahmad et al 2008) and signaling molecules in the rhizosphere to promote plant growth (Walker et al 2003a;Nelson 2004).…”

Section: Discussionmentioning

confidence: 96%

Cyanobacteria-mediated phenylpropanoids and phytohormones in rice (Oryza sativa) enhance plant growth and stress tolerance

Singh

Prabha

Yandigeri

et al. 2011

Antonie van Leeuwenhoek

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Phenylpropanoids, flavonoids and plant growth regulators in rice (Oryza sativa) variety (UPR 1823) inoculated with different cyanobacterial strains namely Anabaena oryzae, Anabaena doliolum, Phormidium fragile, Calothrix geitonos, Hapalosiphon intricatus, Aulosira fertilissima, Tolypothrix tenuis, Oscillatoria acuta and Plectonema boryanum were quantified using HPLC in pot conditions after 15 and 30 days. Qualitative analysis of the induced compounds using reverse phase HPLC and further confirmation with LC-MS/MS showed consistent accumulation of phenolic acids (gallic, gentisic, caffeic, chlorogenic and ferulic acids), flavonoids (rutin and quercetin) and phytohormones (indole acetic acid and indole butyric acid) in rice leaves. Plant growth promotion (shoot, root length and biomass) was positively correlated with total protein and chlorophyll content of leaves. Enzyme activity of peroxidase and phenylalanine ammonia lyase and total phenolic content was fairly high in rice leaves inoculated with O. acuta and P. boryanum after 30 days. Differential systemic accumulation of phenylpropanoids in plant leaves led us to conclude that cyanobacterial inoculation correlates positively with plant growth promotion and stress tolerance in rice. Furthermore, the study helped in deciphering possible mechanisms underlying plant growth promotion and stress tolerance in rice following cyanobacterial inoculation and indicated the less explored avenue of cyanobacterial colonization in stress tolerance against abiotic stress.

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Cyanobacterial cytoskeleton immunostaining: the detection of cyanobacterial cell lysis induced by planktopeptin BL1125

Cited by 13 publications

References 32 publications

UVB Radiation as a Potential Selective Factor Favoring Microcystin Producing Bloom Forming Cyanobacteria

UVB Radiation as a Potential Selective Factor Favoring Microcystin Producing Bloom Forming Cyanobacteria

Extract of Microcystis water bloom affects cellular differentiation in filamentous cyanobacterium Trichormus variabilis (Nostocales, Cyanobacteria)

Cyanobacteria-mediated phenylpropanoids and phytohormones in rice (Oryza sativa) enhance plant growth and stress tolerance

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