Cyanobacterial NOS expression improves nitrogen use efficiency, nitrogen-deficiency tolerance and yield in Arabidopsis

Castello, Fiorella Del; Foresi, Noelia; Nejamkin, Andrés; Lindermayr, Christian; Buegger, Franz; Lamattina, Lorenzo; Correa‐Aragunde, Natalia

doi:10.1016/j.plantsci.2021.110860

Cited by 9 publications

(3 citation statements)

References 73 publications

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“…3), which in turn inhibited C. tinctoria photosynthesis. Appropriate amounts of low N treatment resulted in a chloroplast system similar to that under normal N levels, further demonstrating that the photoassimilation process in plants can proceed relatively easily under low N conditions 25 , which provide evidence for the adaptation of C. tinctoria to a low N environment. Stomatal and nonstomatal limitations in uence the photosynthetic rate.…”

Section: Discussionmentioning

confidence: 74%

Reduced Application of Nitrogen Fertilizer Affects Nitrogen Metabolism of Leaves and Maintains the Production During the Harvesting Stage in Coreopsis tinctoria

Jiang

et al. 2022

Preprint

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Coreopsis tinctoria, as a medicinal plant, has become popular in China in the past decade. However, basic data on NO3− supply related to its production and nitrogen (N) metabolism are lacking. In this study, all plants were grown to four leaves in substrate (vermiculite:perlite = 2:1), which were subsequently transferred to different reducing NO3− solutions. Appropriate N fertilizer application reductions (1/8N-1/2N) maintained the flower number and biomass of Coreopsis tinctoria during 2020–2021. N fertilizer reduction maintained the N assimilation levels of C. tinctoria and increased N use efficiency (NUE), one of the important reasons was that the reduction of N fertilizer did not damage the chlorophyll fluorescence system and photosynthetic system. 1/2N-1/8N treatment also maintained the activities nitrate reductase (NR), nitrite reductase (NIR), glutamine synthase (GS) and glutamate synthase (GOGAT) and the content of total amino acid and soluble protein in leaves. N reduction treatment also increased the levels of tyrosine (Thr), serine (Ser), leucine (Leu), isoleucine (Ile), lysine (Lys) and arginine (Arg) of leaves in C. tinctoria, and further enhances N assimilation ability. Leaf N content was significantly correlated with the activities of NR, NIR, GS, GOGAT and GDH, indicating that N metabolism was effectively regulated by N levels. In conclusion, the 1/8 N level prior to harvest could be one of the strategies for C. tinctoria cultivation because it minimizes planting costs and maintains nitrogen assimilation capacity and productivity.

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Section: Discussionmentioning

confidence: 74%

Reduced Application of Nitrogen Fertilizer Affects Nitrogen Metabolism of Leaves and Maintains the Production During the Harvesting Stage in Coreopsis tinctoria

Jiang

et al. 2022

Preprint

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“…Despite the evidence indicating that NO influences mechanisms underlying nutrient acquisition and utilization, there are scarce measurements of NAE and NUtE in the framework of NO research (e.g., Del Castello et al, 2021 ; Gautam et al, 2021 ). Thus, the precise quantitative influence of NO on them remains mostly unknown.…”

Section: Nue: the Need To Quantify The Impact Of Nomentioning

confidence: 99%

“…The alteration of NO levels could be achieved by the introgression of NO generating/consuming proteins under the control of well-suited promoters, acting under specific nutritional conditions, thus reducing the by-side effects emerging from uncontrolled NO use. Introgression of proteins affecting NO balance, may also result in N metabolism modifications, as shown in Arabidopsis plants expressing SyNOS , from Sinechococcus , which besides to generate NO from arginine, mediates its conversion to nitrate leading to an enhancement of NUE under low N supply (Del Castello et al, 2021 ). In addition, identification of transcription factors and structural proteins acting downstream in the NO signaling network, elicited by specific nutrient stress conditions, may be used for a wide range of approaches to express or edit proteins contributing to traits for enhanced NAE and/or NUtE.…”

Section: Future Directionsmentioning

confidence: 99%

Can NO Signaling and Its Metabolism Be Used to Improve Nutrient Use Efficiency? Toward a Research Agenda

et al. 2022

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Nitric oxide synthase expression in Pseudomonas koreensis MME3 improves plant growth promotion traits

Labarthe,

Maroniche,

Lamattina

et al. 2024

Appl Microbiol Biotechnol

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The development of novel biotechnologies that promote a better use of N to optimize crop yield is a central goal for sustainable agriculture. Phytostimulation, biofertilization, and bioprotection through the use of bio-inputs are promising technologies for this purpose. In this study, the plant growth–promoting rhizobacteria Pseudomonas koreensis MME3 was genetically modified to express a nitric oxide synthase of Synechococcus SyNOS, an atypical enzyme with a globin domain that converts nitric oxide to nitrate. A cassette for constitutive expression of synos was introduced as a single insertion into the genome of P. koreensis MME3 using a miniTn7 system. The resulting recombinant strain MME3:SyNOS showed improved growth, motility, and biofilm formation. The impact of MME3:SyNOS inoculation on Brachypodium distachyon growth and N uptake and use efficiencies under different N availability situations was analyzed, in comparison to the control strain MME3:c. After 35 days of inoculation, plants treated with MME3:SyNOS had a higher root dry weight, both under semi-hydroponic and greenhouse conditions. At harvest, both MME3:SyNOS and MME3:c increased N uptake and use efficiency of plants grown under low N soil. Our results indicate that synos expression is a valid strategy to boost the phytostimulatory capacity of plant-associated bacteria and improve the adaptability of plants to N deficiency. Key points • synos expression improves P. koreensis MME3 traits important for rhizospheric colonization • B. distachyon inoculated with MME3:SyNOS shows improved root growth • MME3 inoculation improves plant N uptake and use efficiencies in N-deficient soil

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Cyanobacterial NOS expression improves nitrogen use efficiency, nitrogen-deficiency tolerance and yield in Arabidopsis

Cited by 9 publications

References 73 publications

Reduced Application of Nitrogen Fertilizer Affects Nitrogen Metabolism of Leaves and Maintains the Production During the Harvesting Stage in Coreopsis tinctoria

Reduced Application of Nitrogen Fertilizer Affects Nitrogen Metabolism of Leaves and Maintains the Production During the Harvesting Stage in Coreopsis tinctoria

Can NO Signaling and Its Metabolism Be Used to Improve Nutrient Use Efficiency? Toward a Research Agenda

Nitric oxide synthase expression in Pseudomonas koreensis MME3 improves plant growth promotion traits

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