Cyanobacterium Synechocystis sp. PCC 6803 lacking adc1 gene produces higher polyhydroxybutyrate accumulation under modified nutrients of acetate supplementation and nitrogen-phosphorus starvation

Utharn, Suthira; Yodsang, Panutda; Incharoensakdi, Aran; Jantaro, Saowarath

doi:10.1016/j.btre.2021.e00661

Cited by 7 publications

(15 citation statements)

References 47 publications

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“…To the best of our knowledge, this is the first evidence of PHA production starting from lipids obtained by microalgae in a cascade approach. Indeed, only carbohydrates from microalgal hydrolysate have been used as carbon source for the production of PHA through bacterial fermentation [ 33 ], or, as an alternative, genetically modified Synechocystis has been used as an eco-friendly alternative in the bioplastic production [ 34 ]. PHAs represent a high added-value product, with their market expected to reach USD 167 million by 2027, at a CAGR of 15.3% from USD 81 million in 2022 ( , accessed on 14 February 2023).…”

Section: Resultsmentioning

confidence: 99%

An Alternative Exploitation of Synechocystis sp. PCC6803: A Cascade Approach for the Recovery of High Added-Value Products

et al. 2023

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Microalgal biomass represents a very interesting biological feedstock to be converted into several high-value products in a biorefinery approach. In this study, the cyanobacterium Synechocystis sp. PCC6803 was used to obtain different classes of molecules: proteins, carotenoids and lipids by using a cascade approach. In particular, the protein extract showed a selective cytotoxicity towards cancer cells, whereas carotenoids were found to be active as antioxidants both in vitro and on a cell-based model. Finally, for the first time, lipids were recovered from Synechocystis biomass as the last class of molecules and were successfully used as an alternative substrate for the production of polyhydroxyalkanoate (PHA) by the native PHA producer Pseudomonas resinovorans. Taken together, our results lead to a significant increase in the valorization of Synechocystis sp. PCC6803 biomass, thus allowing a possible offsetting of the process costs.

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Section: Resultsmentioning

confidence: 99%

An Alternative Exploitation of Synechocystis sp. PCC6803: A Cascade Approach for the Recovery of High Added-Value Products

et al. 2023

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“…After incubation, the calcium acetate (10 mM) was added to the cultures as an organic carbon source and incubated under the same growth conditions for another 10 days. Cultures are then extracted and quantitated for PHB production ( Koch et al, 2019 , 2020 ; Utharn et al, 2021 ). To compare the PHA production rates recorded for isolates, we quantified the PHAs produced by Synechocystis cf.…”

Section: Methodsmentioning

confidence: 99%

“…The most common PHA in prokaryotic cells is poly-β-hydroxybutyrate (PHB), an abundant energy and carbon source storage material ( Balaji et al, 2013 ). Several heterotrophic bacteria, such as Cupriavidus necator and Escherichia coli , can produce PHB by fermentation ( Liebergesell et al, 1994 ; Kichise et al, 1999 ; Abed et al, 2009 ; Wang et al, 2013 ; Ansari and Fatma, 2016 ; Utharn et al, 2021 ). However, these production processes use organic carbon sources mainly derived from crops ( Koch et al, 2020 ).…”

Section: Introductionmentioning

confidence: 99%

Polyhydroxybutyrate-producing cyanobacteria from lampenflora: The case study of the “Stiffe” caves in Italy

et al. 2022

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This study aimed to estimate the green formation lampenflora of “Stiffe” caves in order to evaluate their suitability as an isolation source of cyanobacteria useful for the production of polyhydroxyalkanoates (PHAs). The cave system was chosen as the sampling site due to its touristic use and the presence of high-impact illuminations. The biofilms and the mats of the illuminated walls were sampled. Samples were investigated by 16S rRNA gene analysis and culturable cyanobacteria isolation. The isolated strains were then screened for the production of PHAs under typical culturing and nutritional starvation. Cultures were checked for PHA accumulation, poly-β-hydroxybutyrate (PHB) presence (infrared spectroscopy), and pigment production. The 16S rRNA gene metabarcoding. Highlighted a considerable extent of the pressure exerted by anthropogenic activities. However, the isolation yielded eleven cyanobacteria isolates with good PHA (mainly PHB)-producing abilities and interesting pigment production rates (chlorophyll a and carotenoids). Under normal conditions (BG110), the accumulation abilities ranged from 266 to 1,152 ng mg dry biomass–1. The optimization of bioprocesses through nutritional starvation resulted in a 2.5-fold increase. Fourier transform infrared (FTIR) studies established the occurrence of PHB within PHAs extracted by cyanobacteria isolates. The comparison of results with standard strains underlined good production rates. For C2 and C8 strains, PHA accumulation rates under starvation were higher than Azospirillum brasilense and similar to Synechocystis cf. salina 192. This study broadened the knowledge of the microbial communities of mats and biofilms on the lightened walls of the caves. These findings suggested that these structures, which are common in tourist caves, could be used to isolate valuable strains before remediation measures are adopted.

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“…with a knockout gene (slr512) showed a better high light acclimation compared to the wild-type strain [134]. Metabolic engineering design with target genes involved in the process improved the lipid and ethanol productions [135,136] and proliferative growth and certain contents of intracellular pigments including chlorophyll-a and carotenoids [137,138].…”

Section: Synechocystis Sp: a Model Microorganismmentioning

confidence: 99%

Cyanobacteria: Model Microorganisms and Beyond

Allaf

Peerhossaini

2022

Microorganisms

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In this review, the general background is provided on cyanobacteria, including morphology, cell membrane structure, and their photosynthesis pathway. The presence of cyanobacteria in nature, and their industrial applications are discussed, and their production of secondary metabolites are explained. Biofilm formation, as a common feature of microorganisms, is detailed and the role of cell diffusion in bacterial colonization is described. Then, the discussion is narrowed down to cyanobacterium Synechsystis, as a lab model microorganism. In this relation, the morphology of Synechsystis is discussed and its different elements are detailed. Type IV pili, the complex multi-protein apparatus for motility and cell-cell adhesion in Synechosystis is described and the underlying function of its different elements is detailed. The phototaxis behavior of the cells, in response to homogenous or directional illumination, is reported and its relation to the run and tumble statistics of the cells is emphasized. In Synechsystis suspensions, there may exist a reciprocal interaction between the cell and the carrying fluid. The effects of shear flow on the growth, doubling per day, biomass production, pigments, and lipid production of Synechocystis are reported. Reciprocally, the effects of Synechocystis presence and its motility on the rheological properties of cell suspensions are addressed. This review only takes up the general grounds of cyanobacteria and does not get into the detailed biological aspects per se. Thus, it is substantially more comprehensive in that sense than other reviews that have been published in the last two decades. It is also written not only for the researchers in the field, but for those in physics and engineering, who may find it interesting, useful, and related to their own research.

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Cyanobacterium Synechocystis sp. PCC 6803 lacking adc1 gene produces higher polyhydroxybutyrate accumulation under modified nutrients of acetate supplementation and nitrogen-phosphorus starvation

Cited by 7 publications

References 47 publications

An Alternative Exploitation of Synechocystis sp. PCC6803: A Cascade Approach for the Recovery of High Added-Value Products

An Alternative Exploitation of Synechocystis sp. PCC6803: A Cascade Approach for the Recovery of High Added-Value Products

Polyhydroxybutyrate-producing cyanobacteria from lampenflora: The case study of the “Stiffe” caves in Italy

Cyanobacteria: Model Microorganisms and Beyond

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