Cyanogenic glycoside amygdalin influences functions of human osteoblasts <i>in vitro</i>

Omelka, Radoslav; Kováčová, Veronika; Mondockova, Vladimira; Großkopf, Birgit; Kolesárová, Adriana; Martiniaková, Monika

doi:10.1080/03601234.2020.1852054

Cited by 6 publications

(4 citation statements)

References 61 publications

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“…Amygdalin can decrease cell viability of primary human osteoblast in vitro and negatively affect osteoblasts morphology including mineralization of extracellular matrix and bone resorption. It also modulates the expression of important genes in human osteoblasts associated with osteoblast-specific pathways, oxidative stress, and cell death [ 50 ]. Subacute intramuscular exposure to amygdalin at doses of 0.6 and 3 mg/kg b.w.…”

Section: Physiological and Therapeutic Rolesmentioning

confidence: 99%

The Multiple Actions of Amygdalin on Cellular Processes with an Emphasis on Female Reproduction

2021

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The present review summarizes the current knowledge on the provenance and properties, metabolism and toxicity, mechanism of action, physiological, and therapeutic roles of amygdalin—a molecule present in the seeds of apricot and other plants—with an emphasis on the action of amygdalin on reproductive processes, particularly in the female. Amygdalin influences physiological processes including female reproduction at various regulatory levels via extra- and intracellular signaling pathways regulating secretory activity, cell viability, steroidogenesis, proliferation, and apoptosis. On the other hand, while being metabolized in the body, amygdalin releases significant amounts of cyanide, which may lead to acute health hazard in those individuals who may be at risk. Despite some contradictions in the available data about benefits and toxic effects of amygdalin, its potential applicability at low doses may present a promising tool for regulation of various reproductive and other physiological processes including disease management primarily in cancer phytotherapy, animal production, medicine, and biotechnology. However, further research involving carefully designed dose–response studies is required to overcome the possible side effects of amygdalin and assure its safety as a therapeutic agent.

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Section: Physiological and Therapeutic Rolesmentioning

confidence: 99%

The Multiple Actions of Amygdalin on Cellular Processes with an Emphasis on Female Reproduction

2021

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“…Amygdalin is a natural phyto‐metabolite derived from the aromatic cyanogenic glycosides family found predominantly in the bitter kernels of apricot, almond, apple, and other rosaceous plant seeds (Barakat, 2020; Omelka et al, 2021). It is commonly known as vitamin B17, although it is not a vitamin (Jaswal et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

“…seeds (Barakat, 2020;Omelka et al, 2021). It is commonly known as vitamin B17, although it is not a vitamin (Jaswal et al, 2018).…”

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confidence: 99%

A study on Amygdalin's genotoxicological safety and modulatory activity in human peripheral lymphocytes in vitro

Erikel

Yüzbaşıoğlu

Ünal

2023

Environ and Mol Mutagen

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Amygdalin (AMY), a plant secondary metabolite containing nitrile, is a major component of the seeds of Rosaceae family plants. It is known that this compound has many pharmacological activities such as cancer prevention, antipyretic, and cough suppressant. In this study, the genotoxic and modulatory effects of amygdalin were assessed by chromosomal aberration (CA), sister chromatid exchange (SCE), and cytokinesis‐block micronucleus assay (CBMN) assays using human peripheral lymphocytes (HPLs) in the absence and presence of metabolic activator (S9 mix). Lymphocytes were exposed to various concentrations of amygdalin (0.86, 1.72, 3.43, 6.86, and 13.75 μg/mL) alone and in combination with mitomycin‐C (MMC, 0.20 μg/mL) or cyclophosphamide (CP, 12 μg/mL). The mitotic index (MI), replication index (RI), cytokinesis‐block proliferation index (CBPI), and cytostasis were also evaluated to determine cytotoxicity. Amygdalin alone did not exhibit genotoxic and cytotoxic effects at all the tested concentrations both in the absence and presence of the S9 mix. In contrast, amygdalin significantly reduced the frequencies of CA (especially at 48 h treatments), SCE, and MN (except 0.86 μg/mL in pre‐ and simultaneous treatment) induced by MMC in all the tested concentrations and treatment protocols. It has also considerably decreased CP‐induced CA and SCE frequencies at all the concentrations (except 0.86 μg/mL) in simultaneous treatment. This study demonstrated that amygdalin alone was not genotoxic, on the contrary, it has revealed modulatory effects against chemotherapy agents that induced genomic damage in human lymphocytes, suggesting its chemopreventive potential.

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“…It is reported that the high concentration of AD (10 mg/mL) reduced the viability and size of osteoblasts, decreased extracellular matrix mineralization by down-regulating the COL1A1 and ALPL genes, and enhanced osteoclast formation and bone resorption by up-regulating the TNFSF11 and WNT5A genes while increasing the expression of BGLAP gene. In lower concentrations (0.1 mg/mL), there is almost no effect on functions of human osteoblasts in vitro [ 22 ]. A recent study reported that by using MSC-specific transforming growth factor- (TGF-) β receptor 2 conditional knockout (KO) mice (Tgfbr2Gli1-Cre) and C3H10 T1/2 murine mesenchymal progenitor cells, the migration and differentiation of mesenchymal stem cells (MSCs) accelerated the fracture healing process by AD through the TGF-β/Smad signaling pathway, supporting the use of amygdalin-based therapy for fracture healing [ 23 ].…”

Section: Introductionmentioning

confidence: 99%

Effect on Osteoclast Differentiation and ER Stress Downregulation by Amygdalin and RANKL Binding Interaction

et al. 2022

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Bone diseases such as osteoporosis are the result of osteoclast over-activation. There are many therapeutic agents from natural compounds inhibiting the formation of osteoclast that have been reported and are continuously being interested. Amygdalin (AD) is isolated from seeds of Prunus armeniaca L. which has many pharmaceutical effects; however, the effect of AD on osteoclast formation and function remains unknown. Therefore, the underlying mechanism of AD on RANKL-induced osteoclast in RAW 264.7 cells was investigated. Molecular docking simulation revealed that AD can bind to the active sites of RANKL with negative binding affinities. Through TRAP activity, bone resorption, and migration, AD effectively inhibited osteoclast differentiation and function. Expression of transcription factors, such as NFATc1, c-fos, and osteospecific genes (including dcstamp, acp5, ATP6v0d2, and ctsk results) showed an osteoclast differentiated inhibitory effect by AD treatment. In addition, RANKL-induced activation of MAPK, ER stress, and ROS levels in RANKL-induced osteoclast was significantly inhibited while antioxidant enzymes were recovered in the presence of AD. These results suggest that AD may be a potential candidate derived from natural sources for the treatment of osteoclast bone-related diseases.

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Cyanogenic glycoside amygdalin influences functions of human osteoblasts in vitro

Cited by 6 publications

References 61 publications

The Multiple Actions of Amygdalin on Cellular Processes with an Emphasis on Female Reproduction

The Multiple Actions of Amygdalin on Cellular Processes with an Emphasis on Female Reproduction

A study on Amygdalin's genotoxicological safety and modulatory activity in human peripheral lymphocytes in vitro

Effect on Osteoclast Differentiation and ER Stress Downregulation by Amygdalin and RANKL Binding Interaction

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