Cyathane diterpenes from Chinese mushroom Sarcodon scabrosus and their neurite outgrowth-promoting activity

Synthesis and Protective Effect of New Ligustrazine-Benzoic Acid Derivatives against CoCl2-Induced Neurotoxicity in Differentiated PC12 Cells

“…After drying the organic layer over anhydrous Na 2 SO 4 and evaporating the solvent under vacuum, the crude products were purified by flash chromatography and the target compound was recrystallized from acetone. (3,5,6- [9,[24][25][26] PC12 cells were cultured in RPMI 1640 medium supplemented with 5% (v/v) fetal bovine serum, 10% (v/v) horse serum and 100 U/mL penicillin-streptomycin (Thermo Technologies, New York, NY, USA) at 37 °C in a humidified atmosphere of 5% CO 2 . When cells achieved the desired density of >80% confluency original medium was removed and cells were cultured with the serum-free medium for 14 h. Then the cells were suspended in 1640 medium supplemented with 10% (v/v) fetal bovine serum, and seeded into poly-L-lysine-coated 96-well culture plates at 7 × 10 3 cells/well, differentiated by treated with 50 ng/mL NGF for 48 h. After these, the differentiated PC12 cells were pretreated with various concentrations (60, 30, 15 50 values were defined as the concentration of compounds that produced a 50% proliferation of surviving cells and calculated using the following equation: −pEC 50 = log C max − log 2 × (∑P − 0.75 + 0.25P max + 0.25P min ), Where C max = maximum concentration, ∑P = sum of proliferation rates, P max = maximum value of proliferation rate and P min = minimum value of proliferation rate.…”

“…When cells achieved the desired density of >80% confluency original medium was removed and cells were cultured with the serum-free medium for 14 h. Then the cells were suspended in 1640 medium supplemented with 10% (v/v) fetal bovine serum, and seeded into poly-L-lysine-coated 96-well culture plates at 7 × 10 3 cells/well, differentiated by treated with 50 ng/mL NGF for 48 h. After these, the differentiated PC12 cells were pretreated with various concentrations (60, 30, 15 50 values were defined as the concentration of compounds that produced a 50% proliferation of surviving cells and calculated using the following equation: −pEC 50 = log C max − log 2 × (∑P − 0.75 + 0.25P max + 0.25P min ), Where C max = maximum concentration, ∑P = sum of proliferation rates, P max = maximum value of proliferation rate and P min = minimum value of proliferation rate. [26,27] The PC12 cells culture procedure was similar to that described in section 3.2.1. After pretreatment with the serum-free medium for 14 h, cells were seeded at a concentration of 7 × 10 4 cell/mL in a volume of 0.8 mL on a poly-L-lysine-coated sterile cover slip in 6-well tissue culture plates, and differentiated by treating with NGF (50 ng/mL) for 48 h. Then the differentiated PC12 cells were pretreated with compound 4a (60 µM) for 36 h prior to exposure to CoCl 2 .…”

“…The solvent was removed by distillation under vacuum, and the residue was recrystallized from acetone to produce a light yellow solid (2.39 g, yield: 47.8%), m.p. : 162-163 °C [26].…”

Picrotoxane Sesquiterpene Glycosides and a Coumarin Derivative from Coriaria nepalensis and Their Neurotrophic Activity

“…Compounds (1-3, and 1a-1e) were evaluated for the effect on NGF-induced neurite outgrowth in rat pheochromocytoma PC12 cells according to our previously reported procedures [17,18].…”

“…In our recent effort to search for neurotrophic natural products [13][14][15][16], we employed rat dopaminergic PC12 cells as an in vitro cell model to screen various natural compounds for the effect on nerve growth factor (NGF)-induced neurite outgrowth. The active compounds are expected to be potentially useful for the treatment of Alzheimer's disease and other neurological disorders [17,18]. Here, two new picrotoxane-type sesquiterpene glycosides, nepalactones A (1) and B (2), and one new polyprenyled coumarin, nepalarin (3) (Figure 1), were isolated from the root barks of this plant.…”