Cycle- and function-related changes in lectin binding to human endometrium: a histochemical study with pronase treatment

Kupryjańczyk, Jolanta

doi:10.1007/bf00934521

Cited by 8 publications

(11 citation statements)

References 27 publications

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“…Finally, it should be noted that lectin binding to normal endometrium remained largely unaffected by the cycle-related changes reported earlier [6,16], although ConA did express intraluminal reactivity more frequently during the secretory, rather than during the proliferative, phase of the menstrual cycle.…”

Section: Discussionmentioning

confidence: 60%

Lectin-binding patterns in normal, hyperplastic and neoplastic endometrium: the prognostic value of concanavalin A

et al. 2000

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Lectins are proteins and glycoproteins of non-immune origin which bind specifically to carbohydrate residues, agglutinate cells and/or precipitate complex carbohydrates. Lectin-binding patterns in normal, hyperplastic and neoplastic endometria were studied using four biotinylated lectins (Con A, LCA, e-PHA, l-PHA) and the avidin-biotin-peroxidase technique. Canavalia ensiformis agglutinin (ConA) and Lens culinaris agglutinin (LCA) reacted strongly with the luminal borders and the cytoplasm of epithelial cells but, whilst in normal and benign endometrial tissues the cytoplasmic staining was confined to the apical and the basal aspect of the cells, in endometrial carcinomas and in some atypical hyperplasias lectin binding also occurred in the lateral cytoplasm (Con-A-lat), although in differing proportions of cells. Interestingly, extensive Con-A-lat in the tumour cells was much more frequent in non-endometrioid carcinomas (P<0.05) and was significantly associated with poor histological differentiation (P<0.0001), low oestrogen and progesterone receptor content (P<0.01 and P=0.0001, respectively) and an unfavourable long-term survival (P<0.05). With Phaseolus vulgaris erythroagglutinin (e-PHA) and leucoagglutinin (l-PHA) a linear, rather inconsistent, staining at the level of the basement membranes was observed in the glands: this, also noted with LCA, appeared intact in normal and hyperplastic glands without cytological atypia, and fragmented or absent in malignant glandular structures and in most hyperplastic glands showing cytological atypia. It is concluded that changes in the distribution of lectin-binding molecules in the endometrial cells are associated with the malignant state, whilst the extent of Con-A-lat reflects the biological behaviour of the tumours.

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Section: Discussionmentioning

confidence: 60%

Lectin-binding patterns in normal, hyperplastic and neoplastic endometrium: the prognostic value of concanavalin A

et al. 2000

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“…In general, the literature on Con A suggests that a weak homogeneous staining occurs across the uterine surface, irrespective of the physiological state of the epithelium or type of probes applied (Aoki et al, 1989;Bychkov and Toto, 1987;Duncan et al, 1988;Klentzeris et al, 1991;Kupryjanczyk, 1989). Conversely, the reported decrease in the density of Con A staining across the surface of ''infertile'' versus ''normal fertile'' women (Klentzeris et al, 1991) supports the present findings that indicate a likely role for ␣-mannosyl and ␣-glucosyl residues in preparing a receptive epithelium for blastocyst attachment.…”

Section: Discussionmentioning

confidence: 99%

“…In women, SBA binding to the uterine surface coat across the menstrual cycle was highly variable (Aoki et al, 1989;Kupryjanczyk, 1989;Soderstrom, 1987). However, in one example SBA binding in women was reported to be absent around the early-luteal phase and strongly expressed at the late secretory stage (Jones et al, 1998) suggesting that ␣-D-Gal and ␣-D-GalNAc may play a role in preparing the endometrium for pregnancy as in the macaque (Anderson et al, 1990) rabbit (Bukers et al, 1990) and marmoset.…”

Section: Discussionmentioning

confidence: 99%

“…In the few studies using endometrial samples from women (Aoki et al, 1989;Bychkov and Toto, 1987;Duncan et al, 1988;Kupryjanczyk, 1989), apes (Jones et al, 1998) and monkeys (Anderson et al, 1990) insufficient quantities of surface epithelium from curettings were generally obtained. The range of carbohydrates that typically constitute a plasma membrane glycocalyx has largely been unexplored in primate studies.…”

mentioning

confidence: 99%

“…The range of carbohydrates that typically constitute a plasma membrane glycocalyx has largely been unexplored in primate studies. Although there is convincing evidence in primates to support cyclic changes in glycans in endometrial glands and stroma (Anderson et al, 1986(Anderson et al, , 1990Bychkov and Toto, 1986;Duncan et al, 1988;Horvat, 1993;Jones et al, 1998;Kupryjanczyk, 1989), the changes in carbohydrates associated with a receptive uterine epithelial glycocalyx remain unknown.…”

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confidence: 99%

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Ultrastructural studies of glycan changes in the apical surface of the uterine epithelium during pre-ovulatory and pre-implantation stages in the marmoset monkey

1999

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It has been postulated that carbohydrates are involved in a variety of cell-cell interactions including blastocyst implantation. In primates, there are only limited investigations on the ultrastructural localisation of the cyclic changes in uterine epithelial surface carbohydrates. Our aim was to investigate such changes during the pre-ovulatory and pre-implantation stages of the reproductive cycle in the marmoset monkey.After fixation of endometrial tissues, avidin-ferritin lectin cytochemistry was employed for apical surface glycan detection at the ultrastructural level. Five lectins were used including Canavalia ensiformis (Con A), Lotus tetragonolobus (LTA), Glycine max (SBA), Phytolacca americana (PWM) and Triticum vulgaris (WGA). Morphometry was used to quantitate changes in the intensity of lectin staining by determining the total number of ferritin particles per unit length of membrane. Surface and intra-cytoplasmic vesicles, stained by the lectins, were also examined.Quantitative ferritin assessment showed that 1 day before presumed implantation (days 11 to 12 after ovulation in the marmoset monkey) there was a significant increase in Con A, LTA and SBA staining on the apical uterine epithelial plasma membrane compared to the pre-ovulatory phase and earlier stages of pregnancy (days 4-8 after ovulation). A significant increase in PWM was also detected from early pregnancy to preimplantation stages. All lectins except WGA produced reproducible staining within reproductive cycle groups. The greatest variation and intensity of epithelial surface staining was observed with WGA and the weakest with LTA. The patchy staining with LTA compared with thick coverage by WGA indicated the complexity of the carbohydrate arrangement in the glycocalyx of the uterine surface plasma membrane. Reduction of WGA reactivity after neuraminidase treatment suggested that the lectin binding might be related to the presence of heavily sialylated apical uterine membrane glycoconjugates.This is the first high-resolution study in primates to report quantitative cyclic changes in fucosyl, galactosyl, glucosyl, and mannosyl sugar residues of the apical uterine epithelial glycocalyx. The findings support the concept Abbreviations: DC, NЈN diacetyl chitobiose; Fuc, fucose; Glc, glucose; Man, mannose; GalNAc, N-acetyl galactosamine; GlcNAc, N-acetyl glucosamine; NeuNAc, neuraminic acid (sialyl residues); NЈdase, neuraminidase; sWGA, succinyl wheatgerm; WGA, native wheatgerm; nil, not applied; 0 to 7, lectin staining on a scale from 0 (negative) to 7 (heavy).

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Three-dimensional electron microscopy for endothelial glycocalyx observation using Alcian blue with silver enhancement

et al. 2020

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Glycocalyx (GCX) is a thin layer of negatively charged glycoproteins that covers the vascular endothelial surface and regulates various biological processes. Because of the delicate and fragile properties of this structure, it is difficult to detect GCX morphologically. We established a simple method for a three-dimensional visualization of endothelial GCX using low-vacuum scanning electron microscopy (LVSEM) on formalin-fixed paraffin-embedded (FFPE) sections. Mouse kidney tissue was fixed with 10% buffered formalin containing 1% Alcian blue (ALB) via perfusion and immersion. FFPE sections were observed by light microscopy (LM) and LVSEM, and formalin-fixed epoxy resin-embedded ultrathin sections were observed by transmission electron microscopy (TEM). The endothelial GCX from various levels of kidney blood vessels was stained blue in LM and confirmed as a thin osmiophilic layer in TEM. In LVSEM, the sections stained by periodic acid methenamine silver (PAM) revealed the endothelial GCX as a layer of dense silver-enhanced particles, in both the samples fixed via perfusion and immersion. Correlative light and electron microscopy (CLEM) revealed the fine visible structure of endothelial GCX. This simple method using FFPE samples with ALB will enable the three-dimensional evaluation of endothelial GCX alterations in various human diseases associated with endothelial injury in future studies.

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Cycle- and function-related changes in lectin binding to human endometrium: a histochemical study with pronase treatment

Cited by 8 publications

References 27 publications

Lectin-binding patterns in normal, hyperplastic and neoplastic endometrium: the prognostic value of concanavalin A

Lectin-binding patterns in normal, hyperplastic and neoplastic endometrium: the prognostic value of concanavalin A

Ultrastructural studies of glycan changes in the apical surface of the uterine epithelium during pre-ovulatory and pre-implantation stages in the marmoset monkey

Three-dimensional electron microscopy for endothelial glycocalyx observation using Alcian blue with silver enhancement

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