Cyclic 3′5′ Adenosine Monophosphate in Isolated Bone Cells: Response to Low Concentrations of Parathyroid Hormone12 12

Peck, William A.; Carpenter, J. G.; Messinger, Kirk; Bra, Don De

doi:10.1210/endo-92-3-692

Cited by 108 publications

(37 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…4, the cells were highly sensitive to PTH, significant activation being observed at hormone concentrations as low as 10 nM and a maximal response at 0.5 MM. This is comparable to the sensitivity seen in other isolated bone cell systems (24) and to the PTHresponsive adenylate cyclase in isolated kidney membranes (25). In addition, at no time in the development of the tissue was a response to another hormone observed: CT, insulin, glucagon, and epinephrine had no effect on the adenylate cyclase in day 2 cultures from high-density plates over a concentration range isolated limb bud cells.…”

Section: Resultssupporting

confidence: 86%

See 1 more Smart Citation

Development of parathyroid hormone- and calcitonin-activated adenylate cyclases in embryonic chicken limb and in cultured cells from embryonic chicken limb.

Zull¹,

Krug²,

Abel³

et al. 1978

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

Activation of adenylate cyclase [ATP pyrophosphate-lyase (cyclizing), EC 4.6.1.1] by parathyroid hormone (PTH) and calcitonin was measured as a function of stage of development in embryonic chicken limb buds. Responsiveness to both hormones develops in the tissue at the time when nascent bone is forming. In addition, a temporal sequence of development of hormone response was observed, with a PTHactivated adenylate cyclase appearing earlier than the calcitonin-activated enzyme. The responsiveness to the two hormones was additive, indicating the presence of two receptor populations. Undifferentiated cells obtained from limb buds prior to appearance of hormonal responsiveness were cultured and were found to develop a PTH-activated adenylate cyclase in vitro. However, a calcitonin-stimulated enzyme did not appear in such cultures. The PTH-activated enzyme was found to be similar to that present in bone in regard to its sensitivity to PTH. The enzyme did not respond to other hormones, and myoblast cultures did not develop a PTH-activated adenylate cyclase, indicating that a true bone adenylate cyclase was being measured.

show abstract

Section: Resultssupporting

confidence: 86%

“…The number of plates used for each experiment varied with the time in culture and the density of plating. The range was from 4 plates (35-mm diameter) per membrane preparation to up to 24 plates in the early-time and low-density preparations.…”

Section: Methodsmentioning

confidence: 99%

Development of parathyroid hormone- and calcitonin-activated adenylate cyclases in embryonic chicken limb and in cultured cells from embryonic chicken limb.

Zull¹,

Krug²,

Abel³

et al. 1978

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

show abstract

“…These data are in contrast to previous findings in fetal rat calvaria in which intact b-PTH 1-84 readily stimulates cAMP production (19,20). However, b-PTH 1-84 is extensively degraded to PTH fragments by fetal bone cells whereas oxidized b-PTH 1-84, devoid of biological activity is not metabolized (20).…”

Section: Discussioncontrasting

confidence: 99%

Selective Uptake of the Synthetic Amino Terminal Fragment of Bovine Parathyroid Hormone by Isolated Perfused Bone

Martin¹,

Freitag²,

Conrades³

et al. 1978

J. Clin. Invest.

View full text Add to dashboard Cite

A B S T R A C T Studies from our laboratory have shown that the metabolic clearance rate of carboxy terminal immunoreactive parathyroid hormone (i-PTH) can be accounted for by extraction ofi-PTH by liver and kidney. In contrast, there was no demonstrable hepatic uptake of the synthetic amino terminal bovine PTH fragment and the kidney accounted for only 45% of the metabolic clearance rate of amino terminal i-PTH. This suggested that another major site, presumably bone, played a role in the metabolism of syn b-PTH 1-34. Extraction of i-PTH by isolated perfused bone was studied during infusion of purified bovine PTH (b-PTH) 1-84 or syn b-PTH 1-34. In five studies during infusion of syn b-PTH 1-34 the arteriovenous difference for i-PTH across bone was 36%. In contrast, no significant uptake of carboxy terminal i-PTH was observed in nine studies during infusion of b-PTH 1-84. In addition, when H202-oxidized (biologically inactive) syn b-PTH 1-34 was used no arteriovenous difference was observed. These findings correlated with the ability of these PTH preparations to stimulate cyclic AMP production by the perfused bone. Syn b-PTH 1-34 increased cyclic AMP production at perfusate PTH concentrations of 1-5 ng/ml, whereas b-PTH 1-84 evoked only a minimal response at concentrations of 10-20 ng/ml. We conclude that bone is a major site of metabolism of the amino terminal PTH fragment, syn b-PTH 1-34. In addition, these data suggest that cleavage of the intact hormone, with the production ofamino terminal PTH fragments by peripheral

show abstract

“…1996. Others have generally found highly variable responses to PTH in human-derived cells and, compared to rat-and mouse-derived osteoblast-like cells, the response is usually weak (Peck et al 1973, Auf'mkolk et al 1985, Marie et al 1989, Robey 1995, McCauley et al 1996. Therefore the weak response to PTH seems a general feature in primary cultures of human osteoblast-like cells.…”

Section: Discussionmentioning

confidence: 96%

Three isolation techniques for primary culture of human osteoblast-like cells: A comparison

Jonsson

Frost

Nilsson

et al. 1999

Acta Orthopaedica Scandinavica

View full text Add to dashboard Cite

Cyclic 3′5′ Adenosine Monophosphate in Isolated Bone Cells: Response to Low Concentrations of Parathyroid Hormone12 12

Cited by 108 publications

References 0 publications

Development of parathyroid hormone- and calcitonin-activated adenylate cyclases in embryonic chicken limb and in cultured cells from embryonic chicken limb.

Development of parathyroid hormone- and calcitonin-activated adenylate cyclases in embryonic chicken limb and in cultured cells from embryonic chicken limb.

Selective Uptake of the Synthetic Amino Terminal Fragment of Bovine Parathyroid Hormone by Isolated Perfused Bone

Three isolation techniques for primary culture of human osteoblast-like cells: A comparison

Contact Info

Product

Resources

About