Cyclic bcl-2 gene expression in human uterine endometrium during menstrual cycle

Otsuki, Yoshinori; Ito, Yoshiaki; Akao, Yukihiro; Misaki, O.; Sugimoto, Osamu; Tsujimoto, Yoshihide

doi:10.1016/s0140-6736(94)91051-0

Cited by 177 publications

(86 citation statements)

References 5 publications

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“…Therefore, keratinization does not thoroughly satisfy all the criteria of apoptosis, although cells undergoing keratinization show both chromatin condensation and DNA fragmentation. This finding may support the results of the study edited by Martin [1 1] in which several morphological types of apoptosis were shown to occur in different cell types and tissues, especially, tissues in which typical apoptosis can be observed, besides lymphoid tissues, endometrium [16] and small intestine [5, 6], are rather rare.…”

Section: Resultssupporting

confidence: 87%

The Relationship between Apoptosis and Keratinization in Human Epidermis.

Nakamura¹,

Ito

Matsumoto

et al. 1999

Acta Histochem. Cytochem

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Section: Resultssupporting

confidence: 87%

The Relationship between Apoptosis and Keratinization in Human Epidermis.

Nakamura¹,

Ito

Matsumoto

et al. 1999

Acta Histochem. Cytochem

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“…How the sex steroids regulate bcl-2 gene in one tissue and not another is not known. The progesterone and estrogen receptors also increased in abundance during the proliferative phase of the cycle in parallel with Bcl-2, consistent with a connection between hormonal activity and Bcl-2 expression (Otsuki et al 1994). In rat uterine endometrium undergoing postimplantation decidualization, expression of Bcl-2 and its antagonistic protein, Bax, were regulated by estrogen and progesterone.…”

Section: Uterine Endometriumsupporting

confidence: 65%

“…Bcl-2 has been detected in glandular, stromal and myometrial cells of the human endometrium. In glandular cells Bcl-2 expression peaks in coincidence with the proliferative phase and shows only negligible expression in the secretory phase and during apoptosis (Gompel et al 1994;Otsuki et al 1994). This suggests that bcl-2 gene expression also may be under hormonal control, and may modulate apoptotic involution of the endometrium.…”

Section: Uterine Endometriummentioning

confidence: 99%

Hormonal regulation of physiological cell turnover and apoptosis

Medh

Thompson

2000

Cell Tissue Res

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Physiological cell turnover plays an important role in maintaining normal tissue function and architecture. This is achieved by the dynamic balance of cellular regeneration and elimination, occurring periodically in tissues such as the uterus and mammary gland, or at constant rates in tissues such as the gastrointestinal tract and adipose tissue. Apoptosis has been identified as the prevalent mode of physiological cell loss in most tissues. Cell turnover is precisely regulated by the interplay of various endocrine and paracrine factors, which modulate tissue and cell-specific responses on proliferation and apoptosis, either directly, or by altering expression and function of key cell proliferative and/or death genes. Although recent studies have provided significant information on specific tissue systems, a clearly defined pathway that mediates cell turnover has not yet emerged for any tissue. Several similarities exist among the various tissues with regard to the intermediates that regulate tissue homeostasis, enabling a better understanding of the general mechanisms involved in the process. Here we review the mechanisms by which hormonal and cytokine factors mediate cell turnover in various tissues, emphasizing common themes and tissue-specific differences.

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“…The endometrium undergoes its cycle of proliferation, differentiation, and desquamation, based on levels of either estrogen alone or of both estrogen and progesterone. Since the endometrium undergoes a constant dynamic growth phase, and cyclic variability of other enzyme expressions has been noted in literature (Otsuki et al, 1994) it is possible that P450 might also undergo similar cyclic variability. The goal of this research was to study the expression of CYP3A4 and CYP3A7 in the endometrium and cervix.…”

mentioning

confidence: 96%

Expression and Cyclic Variability of CYP3A4 and CYP3A7 Isoforms in Human Endometrium and Cervix During the Menstrual Cycle

Sarkar¹,

Vadlamuri²,

Ghosh³

et al. 2003

Drug Metab Dispos

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ABSTRACT:CYP3A4, a cytochrome P450 (P450) isoform metabolizes estrogens, whereas CYP3A7, a fetal liver P450 isoform, is involved in estriol biosynthesis. The goal of this study was to evaluate expression of these enzymes in human uterine tissue during the proliferative and secretory phases of the menstrual cycle. Endometrium and cervix specimens were collected from women undergoing hysterectomy (n ‫؍‬ 36). Total mRNA was extracted, quantified, and reverse-transcription polymerase chain reaction (RT-PCR) was carried out using consensus primers for CYP3A. The 453 base pairs PCR product was hybridized with specific internal oligonucleotide probes for CYP3A4 or CYP3A7 end labeled with 32 P ␥-ATP. The relative intensity of hybridization was determined by autoradiography. Expression of CYP3A7 in endometrium was significantly greater (ϳ10-fold) in the proliferative phase compared with the secretory phase (p < 0.05). CYP3A4 expression was comparable between the two phases. Expression of both enzymes was minimal in the cervix. Fluorescence in situ hybridization of paraffinized sections indicated localized expression of CYP3A enzymes in the glandular epithelium as well as the stroma. Comparison of relative fluorescence intensity indicated differential expression of CYP3A7 in various phases of the menstrual cycle. These results suggest that CYP3A expression in the endometrium of premenopausal women may vary depending on the menstrual cycle phase. The P4501 enzymes are a family of hemeproteins that metabolize a number of exogenous and endogenous substrates including steroid hormones (Nelson et al., 1993;Sarkar et al., 1997). Of these, the CYP3A subfamily of enzymes has been established as the most abundant P450 in humans, representing about 30% of all hepatic P450 (Slaughter et al., 1995). So far three major isoforms have been identified in humans, CYP3A4, CYP3A5, and CYP3A7, of which CYP3A4 is the predominant hepatic isoform (Sarkar et al., 1997) and CYP3A7 is a human fetal liver form, first isolated as a 16-␣-dehydroepiandrosterone-sulfate (DHEA-s) hydroxylase (Kitada et al., 1987). The role of several P450 isoforms in catalyzing the hydroxylation of cortisol, testosterone, androstenedione, estradiol, and progesterone has been established (Berliner et al., 1956;Lipman et al., 1962;Tukey et al., 1985;Guengerich, 1988). However, it is not clear whether these reactions in the liver have any physiological importance, if any, or simply serve as accessory elimination pathways. Recent evidence from our lab has shown that the uterus, specifically endometrium and cervix, expressed differential levels of CYP1A1 and CYP1B1 mRNA (Satya et al., 1998). These observations suggest that extrahepatic P450 may participate in tissue-specific biotransformations, which might be of physiologic relevance.While several P450 isoforms have been indicated in estrogen biotransformation, CYP3A4 is probably one of the major isoforms involved in several pathways (Aoyama et al., 1990;Brian et al., 1990). CYP3A7 has been found to catalyze 16-␣-hydroxylati...

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Cyclic bcl-2 gene expression in human uterine endometrium during menstrual cycle

Cited by 177 publications

References 5 publications

The Relationship between Apoptosis and Keratinization in Human Epidermis.

The Relationship between Apoptosis and Keratinization in Human Epidermis.

Hormonal regulation of physiological cell turnover and apoptosis

Expression and Cyclic Variability of CYP3A4 and CYP3A7 Isoforms in Human Endometrium and Cervix During the Menstrual Cycle

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