Cyclic Change in Hypothalamic Lactic Dehydrogenase Activity in the Adult Female Rat

Morishita, Hajime; Nagamachi, Norio; Kawamoto, M.; Yoshida, Junichi; Ozasa, Teruyasu; Adachi, Haruo

doi:10.1530/acta.0.0710226

Cited by 10 publications

(8 citation statements)

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“…Such variations are similar to those reported about oxygen uptake [Moguilevsky et al, 1968;Schiaffini et al, 1969;Schiaffini et al, 1970;Schiaffini and Marin, 1971] and C 0 2 production from labelled glu cose [Scacchi et al, 1973], and agree well with the changes in total LDH activity of the HYP found by Morishita et al [1972].…”

Section: Discussionsupporting

confidence: 90%

“…One of these changing enzymes is lac tate dehydrogenase (LDH) [Morishita et al, 1972;Packman et al, 1976]. However, little Received: February 22, 1978 Accepted after revision: August 16, 1978 attention has been paid to the nature of LDH present in the HYP: LDH in most tissues is a tetrameric molecule composed of 2 types of subunits (A or M and B or H) that are able to combine into the 5 molecular form s-iso enzymes (ISO) -of LDH [Cahn et al, 1962;Goodfriend and Kaplan, 1964], Since the A/B subunits ratio of LDH extracted from such estrogen target tissues as human endometrium [.Spellman and Fottrell, 1972], rat uterus and anterior pituitary (PIT) Anda et al, 1976b] seems to be dependent on the levels of these hormones, it was considered of interest to study the LDH…”

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confidence: 99%

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Lactate Dehydrogenase Isoenzymes of the Hypothalamus, Limbic Structures, and the Anterior Pituitary during the Estrous Cycle

Oaknin¹,

Alonso²,

Prieto³

et al. 1979

Neuroendocrinology

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Lactate dehydrogenase (LDH) and its isoenzyme (ISO) pattern were measured in the hypothalamus (HYP), amygdala (AMYG), hippocampus (HIP), cerebral cortex and anterior pituitary (PIT) of adult female rats sacrificed at different estrous cycle phases. The HYP, the AMYG and the PIT showed their maximal LDH values at proestrus and the lowest at diestrus. Those of the HIP showed the opposite pattern. The study of the ISO distribution pattern indicated that the cyclic variations of pituitary LDH activity were mainly due to changes of the ISO rich in A subunits, while in the B type they remained constant. By contrast, the cyclic changes of total LDH activity of all the brain structures studied were due to well balanced variations of both A and B subunits.

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Section: Discussionsupporting

confidence: 90%

mentioning

confidence: 99%

Lactate Dehydrogenase Isoenzymes of the Hypothalamus, Limbic Structures, and the Anterior Pituitary during the Estrous Cycle

Oaknin¹,

Alonso²,

Prieto³

et al. 1979

Neuroendocrinology

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“…The stages of the 4-day estrous cycle are defined as estrus, 2 days of diestrus and proestrus. Under the conditions of our laboratory, the critical period is between 5.00 and 8.00 h on the day of proestrus [6], and the time of ovulation is confirmed to be between 18.00 and 21.00 h in the early period of the day following proestrus. The results were statistically evaluated by Student's t test.…”

Section: Animalsmentioning

confidence: 62%

Reproductive Function following Advanced Ovulation Induced by Synthetic Luteinizing Hormone-Releasing Hormone in Rats

Morishita¹,

Mikami²,

Nakago³

et al. 1983

Gynecol Obstet Invest

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Reproductive function following advanced ovulation induced by a single injection of 1 µg synthetic luteinizing hormone-releasing hormone (LH-RH) has been studied in rats. While advanced ovulation induced by an injection of the agent on the day of proestrus was followed by pregnancies occurring as frequently as those in spontaneous ovulation group, incidences of mating behavior, fertilization, and pregnancy were considerably reduced in the advanced ovulation group when induced by an injection of synthetic LH-RH during the early period of diestrus 2. The mean volume of primary oocytes of the three largest follicles at 1.00 h on the day of diestrus 2 was significantly smaller than that at 1.00 h on the day of proestrus. However, shortly before ovulation, the mean volume of secondary oocytes in the preovulatory follicles from rats treated with synthetic LH-RH at 1.00 h on the day of diestrus 2 was approximately the same as that in the preovulatory follicles from rats treated with the agent at 1.00 h on the day of proestrus. Shortly after ovulation the number of mitotic cumulus cells around the oviductal ova was significantly greater in advanced ovulation group induced by synthetic LH-RH injection during the early period of diestrus 2 than in spontaneous ovulation group. Furthermore, when examined 13 h after ovulation, the layers of cumulus cells were thicker, and cumulus cells were more tightly arranged around the ova in the advanced ovulation group induced by injection of synthetic LH-RH during the early period of diestrus 2 than those in the group with spontaneous ovulation. These results suggest that the cumulus-oocyte complex from advanced ovulation group induced by injection of synthetic LH-RH in the early period of diestrus 2 is less potent in fertilizability than that from spontaneous ovulation group, leading to a reduction in the rate of subsequent pregnancy.

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“…Animals were exsanguinated. The brain was immediately removed from the cranium, and the anterior hypothalamus was dissected out as a block of tissue, according to the limits described by Morishita et al [7], and was stored at -2 0 °C until assayed. Only the tissue of the anterior hypothalamus taken at 5.00-6.00 h on the day of proestrus was weighed on an ana lytical balance.…”

Section: Treatment O F Animalsmentioning

confidence: 99%

An Autoradiographic Study of Changes in the Uptake of Tritiated Estradiol in the Anterior Hypothalamus during the Estrous Cycle of the Rat

Morishita¹,

Kuroiwa²,

Higuchi³

et al. 1986

Gynecol Obstet Invest

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In cycling rats exposed to a controlled light schedule of 12 h of light daily from 22.00 to 10.00 h the concentration of estradiol in the anterior hypothalamus at 5.00–6.00 h on the day of proestrus was significantly higher than those at 0.00–1.00 h on day 1 of diestrus and estrus, and was about twofold higher than serum estradiol concentration, which reached a peak at 5.00–6.00 h on the same day. Autoradiographically, the uptake of tritiated estradiol by the nuclei of neurons in the nucleus (n.) preopticus, pars suprachiasmatica (POSC), n. preop-ticus periventricularis (POP), and anterior part of n. anterior hypothalami (AAH) was significantly higher at 1.00 h on the day of diestrus 1 than at 6.00 h on the day of proestrus and at 1.00 h on the day of estrus. These results suggest that the POSC, POP and AAH may be target sites in the anterior hypothalamus of estradiol for its feedback action.

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Cyclic Change in Hypothalamic Lactic Dehydrogenase Activity in the Adult Female Rat

Cited by 10 publications

References 0 publications

Lactate Dehydrogenase Isoenzymes of the Hypothalamus, Limbic Structures, and the Anterior Pituitary during the Estrous Cycle

Lactate Dehydrogenase Isoenzymes of the Hypothalamus, Limbic Structures, and the Anterior Pituitary during the Estrous Cycle

Reproductive Function following Advanced Ovulation Induced by Synthetic Luteinizing Hormone-Releasing Hormone in Rats

An Autoradiographic Study of Changes in the Uptake of Tritiated Estradiol in the Anterior Hypothalamus during the Estrous Cycle of the Rat

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