Cyclic changes in phospholipid content and composition in human endometrium during the menstrual cycle

Montané, Joel; Pérez-Ballester, B.

doi:10.1530/jrf.0.0730317

Cited by 6 publications

(5 citation statements)

References 15 publications

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“…3 ). Previous research demonstrates total phospholipid content of the endometrium is increased in the luteal phase by 26% relative to the periovulatory time-period 27 . A 10-fold increase of phospholipase A 2 in endometrial tissue has previously been identified in the luteal phase 28 .…”

Section: Discussionmentioning

confidence: 93%

Menstrual cycle rhythmicity: metabolic patterns in healthy women

Draper

Duisters

Weger

et al. 2018

Sci Rep

151

130

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The menstrual cycle is an essential life rhythm governed by interacting levels of progesterone, estradiol, follicular stimulating, and luteinizing hormones. To study metabolic changes, biofluids were collected at four timepoints in the menstrual cycle from 34 healthy, premenopausal women. Serum hormones, urinary luteinizing hormone and self-reported menstrual cycle timing were used for a 5-phase cycle classification. Plasma and urine were analyzed using LC-MS and GC-MS for metabolomics and lipidomics; serum for clinical chemistries; and plasma for B vitamins using HPLC-FLD. Of 397 metabolites and micronutrients tested, 208 were significantly (p < 0.05) changed and 71 reached the FDR 0.20 threshold showing rhythmicity in neurotransmitter precursors, glutathione metabolism, the urea cycle, 4-pyridoxic acid, and 25-OH vitamin D. In total, 39 amino acids and derivatives and 18 lipid species decreased (FDR < 0.20) in the luteal phase, possibly indicative of an anabolic state during the progesterone peak and recovery during menstruation and the follicular phase. The reduced metabolite levels observed may represent a time of vulnerability to hormone related health issues such as PMS and PMDD, in the setting of a healthy, rhythmic state. These results provide a foundation for further research on cyclic differences in nutrient-related metabolites and may form the basis of novel nutrition strategies for women.

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Section: Discussionmentioning

confidence: 93%

Menstrual cycle rhythmicity: metabolic patterns in healthy women

Draper

Duisters

Weger

et al. 2018

Sci Rep

151

130

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“…4). The phosphatidyl inositol content of human endometrium is approximately 10-1 5 nmol lipid phosphorus/mg protein (Montane & Perez-Ballester, 1985). The maximum amount of hydrolysis under the conditions described is therefore unlikely to exceed 10%.…”

Section: Suhstrnte Concentrationmentioning

confidence: 81%

“…Our earlier studies (Bonney et al, 1987) have focussed on the hypothesis that the major source of free arachidonic acid for prostaglandin synthesis by human endometrium is phosphatidylcholine which is the predominant phospholipid in most tissues, including human endometrium (Montane & Perez-Ballester, 1985) and considered to be the major substrate for PLA2. The profile of PLA2(i) activity in the endometrium through the menstrual cycle, as demonstrated by our studies (Bonney, 1985;Bonney et al, 1987) does not complement the pattern of endometrial PG concentrations reported by other workers (Downie et al, 1974;Singh et al, 1975).…”

Section: Discussionmentioning

confidence: 99%

“…Michaelis-Menten kinetics have therefore not been applied to the data presented here. The phosphatidyl inositol content of human endometrium is approximately 10-1 5 nmol lipid phosphorus/mg protein (Montane & Perez-Ballester, 1985). Thus the substrate concentration of 40 nmol/tube (1 ,umol/mg protein) subsequently used for the assay was substantially in excess of endogenous PI content.…”

Section: Suhstrnte Concentrationmentioning

confidence: 99%

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Phospholipase C Activity in Human Endometrium: Its Significance in Endometrial Pathology

Bonney

1987

Clinical Endocrinology

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Phospholipase C activity was measured in human endometrium using an assay based on the release of total labelled water soluble products (inositol, inositol phosphates) from L-3-phosphatidyl-[2-3H] inositol. The enzyme was shown to be calcium dependent and to have an optimum pH of 5.5. There was no difference between proliferative phase and secretory phase endometrium with respect to phospholipase C activity either in women with normal menstrual blood loss (proliferative phase: 3.7 +/- 0.7 (mean +/- SD), secretory phase: 4.5 +/- 2.0 nmol/mg protein/min) or in those complaining of severe menorrhagia (proliferative phase: 5.8 +/- 2.8, secretory phase: 7.0 +/- 2.8 nmol/mg protein/min). However, women complaining of severe menorrhagia had significantly higher endometrial phospholipase C activity than those in the normal group (P less than 0.01 and P less than 0.02 for proliferative and secretory phases respectively). Endometrial phospholipase C activity was also elevated in the presence of other gynaecological disorders, e.g. dysmenorrhoea, adenocarcinoma of the cervix and endometrial hyperplasia. The results indicate that phospholipase C activity in human endometrium is not related to the stage of the menstrual cycle but that in the presence of menorrhagia and other gynaecological disorders, activity is increased. Phospholipase C could be implicated in the generation of arachidonic acid for prostaglandin synthesis which may in turn be associated with these abnormalities.

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“…PA(37:4) can affect cell growth, proliferation, migration and invasion by involvement in cell signaling 46 . Studies have shown that after estrogen stimulation, the PA level in the human endometrium is significantly reduced, which may be related to the participation in the de novo lipid biosynthesis pathway 47 . Consistent with the reports in previous studies, serum PS and PA levels of EP patients showed a downward trend in our study, but the detailed mechanism needs to be further explored and explained.…”

Section: Discussionmentioning

confidence: 99%

A serum lipidomics study for the identification of specific biomarkers for endometrial polyps to distinguish them from endometrial cancer or hyperplasia

Yan

Zhao

Wei

et al. 2022

Intl Journal of Cancer

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Endometrial diseases, including endometrial polyps (EP), endometrial cancer (EC) and endometrial hyperplasia (EH), are common gynecological diseases that affect women of childbearing and perimenopausal age. Clinically, biopsy or imaging methods are usually used to screen and diagnose these diseases; however, due to the invasiveness and heterogeneity of these tests, a noninvasive, convenient, objective and accurate biomarker is needed for the differential diagnosis of EP, EC or EH. In the present study, serum samples from 326 patients with endometrial diseases and 225 healthy volunteers were analyzed using nontargeted lipidomics. A combination of multivariate and univariate analyses was used to identify and qualify six, eight and seven potential biomarkers in the sera from patients with EP, EC and EH, respectively. Using a logistic regression algorithm and receiver operating characteristic (ROC) curve analysis, a biomarker panel including four specific EP biomarkers, 6‐keto‐PGF1α, PA(37:4), LysoPC(20:1) and PS(36:0), showed good classification and diagnostic ability in distinguishing EP from EC or EH. The biomarker panel for distinguishing EP from EC yielded an area under the curve (AUC) of 0.915, sensitivity of 100% and specificity of 72.41%, while that for distinguishing EP from EH yielded an AUC of 1.000, sensitivity of 100% and specificity of 100%. The two diagnostic models also showed good diagnostic abilities in the validation set. Therefore, this biomarker panel can be used as a rapid diagnostic method to assist in imaging examinations and provide a reference for clinicians in the identification and diagnosis of endometrial diseases.

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Cyclic changes in phospholipid content and composition in human endometrium during the menstrual cycle

Cited by 6 publications

References 15 publications

Menstrual cycle rhythmicity: metabolic patterns in healthy women

Menstrual cycle rhythmicity: metabolic patterns in healthy women

Phospholipase C Activity in Human Endometrium: Its Significance in Endometrial Pathology

A serum lipidomics study for the identification of specific biomarkers for endometrial polyps to distinguish them from endometrial cancer or hyperplasia

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