Cyclic enzymatic amplification method for highly sensitive detection of nuclear factor-kappa B

Du, Kun; Wu, Jinze; Pan, Anqiang; Li, Dingyun; Cui, Liang; Peng, Cheng

doi:10.1016/j.aca.2019.03.059

Cited by 9 publications

(5 citation statements)

References 36 publications

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“…By applying PCR amplification, MBs have also been broadly applied in diagnosis such as recognition of human pathogens and detection of disease-related to SNPs and alleles. Novel approaches, such as enzymatic amplification [151] and quenching at metal areas [152], have been emerged and In the future, the efforts for allowing molecular study methods based on MBs method will absolutely be advanced. The significance of various molecules detection causes the development of point-of-care study methods extremely important.…”

Section: Conclusion and Future Perspectivesmentioning

confidence: 99%

Molecular beacon strategies for sensing purpose

Bidar

Amini

Oroojalian

et al. 2021

TrAC Trends in Analytical Chemistry

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Section: Conclusion and Future Perspectivesmentioning

confidence: 99%

Molecular beacon strategies for sensing purpose

Bidar

Amini

Oroojalian

et al. 2021

TrAC Trends in Analytical Chemistry

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“…In order to detect NF-KB P50, Du et al applied Exo III assisted circulation to amply the fluorescence signal, achieved the sensitive detection of NF-KB P50 with a limit of detection as low as 45.6 pM. Furthermore, they applied this method for inhibitor screening and cell sample detection [ 132 ]. Adenosine is a kind of endogenous nucleoside in human cells.…”

Section: Application Of Csa For Fluorescent Biosensorsmentioning

confidence: 99%

Recent advances of fluorescent biosensors based on cyclic signal amplification technology in biomedical detection

Fan

Chen

et al. 2021

J Nanobiotechnol

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The cyclic signal amplification technology has been widely applied for the ultrasensitive detection of many important biomolecules, such as nucleic acids, proteins, enzymes, adenosine triphosphate (ATP), metal ions, exosome, etc. Due to their low content in the complex biological samples, traditional detection methods are insufficient to satisfy the requirements for monitoring those biomolecules. Therefore, effective and sensitive biosensors based on cyclic signal amplification technology are of great significance for the quick and simple diagnosis and treatment of diseases. Fluorescent biosensor based on cyclic signal amplification technology has become a research hotspot due to its simple operation, low cost, short time, high sensitivity and high specificity. This paper introduces several cyclic amplification methods, such as rolling circle amplification (RCA), strand displacement reactions (SDR) and enzyme-assisted amplification (EAA), and summarizes the research progress of using this technology in the detection of different biomolecules in recent years, in order to provide help for the research of more efficient and sensitive detection methods. Graphical Abstract

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“…Xie’s group constructed a fluorescence strategy for detecting TFs by engaging the interconverting conformations of TF-specific dsDNA; the binding of TF quickly converted dsDNA from the “OFF-state” to the “ON-state”, and the limit of detection (LOD) was 0.23 pM . The aforementioned flexible assays enable the quantitative detection of TFs with superior specificity and potency, but the response of TFs to the dsDNA usually occurs at a 1:1 stoichiometric ratio, which means that a TF can only trigger one dsDNA to generate a detection signal . Therefore, it is still challenging for sensitive TF detection because of the lack of an effective signal amplification manner.…”

Section: Introductionmentioning

confidence: 99%

“…13 The aforementioned flexible assays enable the quantitative detection of TFs with superior specificity and potency, but the response of TFs to the dsDNA usually occurs at a 1:1 stoichiometric ratio, which means that a TF can only trigger one dsDNA to generate a detection signal. 14 Therefore, it is still challenging for sensitive TF detection because of the lack of an effective signal amplification manner. More importantly, the TF sensing systems primarily rely on single-modal readout, which encounters the predicaments of poor external antiinterference ability, false-positive signals, and inaccuracy due to different operators and variant experimental environments, thereby affecting the accuracy of the analysis to a certain extent.…”

Section: ■ Introductionmentioning

confidence: 99%

Thermodynamic Allosteric Switch-Actuated 3D DNA Nanomachine for Ultrasensitive Electrochemical/Fluorescent Dual-Mode Biosensing of a Transcription Factor

Wang,

Zhang,

Liu

et al. 2024

ACS Appl. Bio Mater.

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Herein, we reported an innovative thermodynamic allosteric switch-actuated 3D DNA nanomachine for selective, sensitive, and accurate electrochemical (EC)/fluorescent (FL) dual-mode biosensing of a microphthalmia-associated transcription factor (MITF). The thermodynamic allosteric switch was ingeniously customized as a hairpin probe (HP) that was in dynamic equilibrium but rapidly interconverting conformations. At the "inactive state", the MITF-binding region and the switch part were "sequestered". Upon the introduction of MITF, an MITF-HP complex promptly formed, and the equilibrium of HP thermodynamically inclined from the "inactive state" toward the "active state" conformation. Immediately, the exposed switch on HP effectively actuated the 3D DNA nanomachine and synchronously produced the restriction site for Nb.BbvCI nicking endonuclease. After the autonomous conveying of the 3D DNA nanomachine by means of the high-efficiency circularly nicking endonuclease signal amplification (NESA), not only was MB-S1 in the supernatant used for FL measurements but also MB-SP/MNs/S2 in the precipitate was adapted for EC analysis, significantly improving the utilization of output products derived from the 3D DNA nanomachine. Accordingly, benefiting from the efficient DNA nanomachine signal amplification manner and the self-calibration function of a dual-mode bioassay, the constructed biosensor exhibits superior sensitivity and accuracy for MITF determination.

show abstract

Cyclic enzymatic amplification method for highly sensitive detection of nuclear factor-kappa B

Cited by 9 publications

References 36 publications

Molecular beacon strategies for sensing purpose

Molecular beacon strategies for sensing purpose

Recent advances of fluorescent biosensors based on cyclic signal amplification technology in biomedical detection

Thermodynamic Allosteric Switch-Actuated 3D DNA Nanomachine for Ultrasensitive Electrochemical/Fluorescent Dual-Mode Biosensing of a Transcription Factor

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