Cyclic ferrocenylnaphthalene diimides as a probe for electrochemical telomerase assay

Kaneyoshi, Shuma; Eguchi, Nao; Fujimoto, Kazuhisa; Fujii, Satoshi; Sato, Shinobu; Takenaka, Shigeori

doi:10.1016/j.jinorgbio.2022.111746

Cited by 5 publications

(3 citation statements)

References 19 publications

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“…2.1 Materials 1-4 were synthesized according to a previous report [8][9][10]. Tris(hydroxymethyl)aminomethane and hydrochloric acid (Tris-HCl, pH 7.2) was purchased from Merck KGaA Sigma-Aldrich Japan K. K (Tokyo, Japan).…”

Section: Methodsmentioning

confidence: 99%

“…1 and 2 were synthesized as electrochemical four strand detection reagents and analyzed for interaction with telomeric four-strand DNA [8]. However, telomeric fourstrand DNA forms a hybrid structure in the presence of potassium ions, which is different from the topology of RG-1 in COVID-19 [5,6].…”

Section: Binding Behavior Of 1-3 With Rg-1mentioning

confidence: 99%

“…This G4 site is also expected to be a mutation‐insensitive target for the detection of COVID‐19, but to our knowledge there are no reported examples of studies aimed at detection. We have already shown that cyclic naphthalene diimides 1 and 2 (Figure 1) bind strongly to G4 and could be useful for electrochemical detection targeting G4 by using an electrode immobilized with G4 [8].…”

Section: Introductionmentioning

confidence: 97%

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Electrochemical Method for G‐quartet RNA Detection of COVID‐19 Based on Cyclic Ferrocenylnaphthalene Diimide

et al. 2022

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To develop an electrochemical detection method for COVID-19, we have investigated the interaction between RG-1, a characteristic parallel four-strand RNA component of the COVID-19 genome, and cyclic ferrocenylnaphthalene diimides carrying different linker lengths 1 and 2, which bind to RG-1 more strongly than cyclic naphthalene diimide 3 that lacks a ferrocene moiety. In particular, the binding affinity of 1 having short one showed the highest binding affinity to RG-1, and the redox current of 1 increased in the presence of RG-1 using a glassy carbon electrode. This increasing current of 1 in the presence of c-myc as a parallel G4 DNA or single-stranded RNA was lower than that in the presence of RG-1. Several RG-1 was electrochemically detected at the nanomolar level by 1.

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Section: Methodsmentioning

confidence: 99%