Cyclic GMP and cyclic GMP phosphodiesterase in the cell cycle of <i>Tetrahymena pyriformis</i>

Graves, M.G.; Dickinson, J. Richard; Swoboda, B.E.P.

doi:10.1016/0014-5793(76)80677-1

Cited by 20 publications

(9 citation statements)

References 5 publications

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“…Furthermore, we have observed that in selection synchronized Tetrahymena pyriformis cyclic GMP is highest during cell division and then declines progressively to its lowest level at a point immediately before cell division [2] . In this experiment cyclic GMP falls to minima which immediately precede the cell divisions.…”

Section: Resultsmentioning

confidence: 83%

“…It is probable that cyclic GMP falls rapidly in hypoxia-synchronized Tetrahymena pyriformis after division, giving the characteristic 'spike' pattern observed because the cells have no GI phase in this cell cycle system [3]. If GI were present, the decline of cyclic GMP would presumably be more gradual as it is in the natural cell cycle [2] .…”

Section: Resultsmentioning

confidence: 99%

“…The extraction and assay of cyclic GMP were performed as described previously [2] without modification.…”

Section: Extraction and Assay Of Cyclic Gmpmentioning

confidence: 99%

“…Cyclic GMP phosphodiesterase was assayed as described previously [2], at a substrate concentration of 0.36 mM.…”

Section: Assay Of Cyclic Gmp Phosphodiesterasementioning

confidence: 99%

“…Using selection synchronized cultures of Tetrahymena pyriformis, we have reported on the modulation of cyclic AMP and cyclic GMP in the natural cell cycle [1,2] . We have recently employed the technique of a single hypoxic shock to induce cell synchrony [3].…”

Section: Introductionmentioning

confidence: 99%

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Cyclic GMP metabolism in Tetrahymena pyriformis synchronized by a single hypoxic shock

1977

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Section: Resultsmentioning

confidence: 83%

Section: Resultsmentioning

confidence: 99%

“…The extraction and assay of cyclic GMP were performed as described previously [2] without modification.…”

Section: Extraction and Assay Of Cyclic Gmpmentioning

confidence: 99%

“…Cyclic GMP phosphodiesterase was assayed as described previously [2], at a substrate concentration of 0.36 mM.…”

Section: Assay Of Cyclic Gmp Phosphodiesterasementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Cyclic GMP metabolism in Tetrahymena pyriformis synchronized by a single hypoxic shock

1977

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Intracellular pH changes during the cell cycle in Tetrahymena

Gillies

Deamer

1979

Journal Cellular Physiology

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The equilibrium distribution of 5,5-dimethyloxazoladine 2,4-dione (DMO) between intra- and extracellular volume was used to estimate intracellular pH (pHi) in Tetrahymena pyiformis. In control experiments, DMO was found to equilibrate rapidly in response to a pH gradient. Under normal growth conditions, pHi was constant over a finite range of external pH, being maintained near pH 7.1 over the external pH range 5.2 to 7.3. This same range of external pH was also optimal for growth. pHi was monitored during the cell cycle of a synchronous population of T. pyriformis GL. The cells were synchronized either by starvation/refeeding or heat shock. Under both conditions, there were two alkaline shifts of approximately 0.4 pH units per cell cycle. These shifts in pH retained a constant remporal relationship to S phase and were not affected by changes in the time, duration, or magnitude of cytokinesis.

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