Cyclic-GMP enhances light-induced excitation and induces membrane currents in Drosophila retinal photoreceptors

Bacigalupo, Juan; Bautista, DM; Brink, DL; Hetzer, JF; O'Day, PM

doi:10.1523/jneurosci.15-11-07196.1995

Cited by 35 publications

(21 citation statements)

References 33 publications

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“…For example, cGMP produced by a receptor guanylyl cyclase is the primary signal molecule in vertebrate phototransduction (Kramer and Molokanova, 2001). In Drosophila, cGMP appears to play a modulatory role in phototransduction and olfaction, rather than being involved in the primary transduction pathway (Bacigalupo et al, 1995;Morton and Hudson, 2002). In the silkmoth (Bombyx mori), soluble and particulate guanylyl cyclase activity was measured in the antennae, and in Manduca the receptor-like guanylyl cyclase MsGC-I was detected in olfactory receptor neurons (Nighorn et al, 2001).…”

Section: Biochemical Properties Ofmentioning

confidence: 99%

Preliminary characterization of two atypical soluble guanylyl cyclases in the central and peripheral nervous system ofDrosophila melanogaster

Langlais

Stewart

Morton

2004

Journal of Experimental Biology

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SUMMARYConventional soluble guanylyl cyclases form α/β heterodimers that are activated by nitric oxide (NO). Recently, atypical members of the soluble guanylyl cyclase family have been described that include the ratβ2 subunit and MsGC-β3 from Manduca sexta. Predictions from the Drosophila melanogaster genome identify three atypical guanylyl cyclase subunits: Gyc-88E (formerly CG4154), Gyc-89Da (formerly CG14885) and Gyc-89Db (formerly CG14886). Preliminary data showed that transient expression of Gyc-88E in heterologous cells generated enzyme activity in the absence of additional subunits that was slightly stimulated by the NO donor sodium nitroprusside (SNP) but not the NO donor DEA-NONOate or the NO-independent activator YC-1. Gyc-89Db was inactive when expressed alone but when co-expressed with Gyc-88E enhanced the basal and SNP-stimulated activity of Gyc-88E, suggesting that they may form heterodimers in vivo. Here,we describe the localization of Gyc-88E and Gyc-89Db and show that they are expressed in the embryonic and larval central nervous systems and are colocalized in several peripheral neurons that innervate trachea, basiconical sensilla and the sensory cones in the posterior segments of the embryo. We also show that there are two splice variants of Gyc-88E that differ by seven amino acids, although no differences in biochemical properties could be determined. We have also extended our analysis of the NO activation of Gyc-88E and Gyc-89Db, showing that several structurally unrelated NO donors activate Gyc-88E when expressed alone or when co-expressed with Gyc-89Db.

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Section: Biochemical Properties Ofmentioning

confidence: 99%

Preliminary characterization of two atypical soluble guanylyl cyclases in the central and peripheral nervous system ofDrosophila melanogaster

Langlais

Stewart

Morton

2004

Journal of Experimental Biology

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“…Mutations in norpA and glass affect the visual input pathway. The norpA gene encodes a phospholipase C (PLC) required for inositol signaling within the phototransduction pathway, in which NORPA is activated by photoactivated rhodopsin via a G protein complex (7,8). norpA is primarily expressed within the rhabdomeres of the compound eye and the retina and axons of photoreceptor cells (9, 10) and even some tissues lacking phototransduction (11).…”

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confidence: 99%

Seasonal behavior in Drosophila melanogaster requires the photoreceptors, the circadian clock, and phospholipase C

Collins

Rosato

Kyriacou

2004

Proc. Natl. Acad. Sci. U.S.A.

118

120

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Drosophila melanogaster locomotor activity responds to different seasonal conditions by thermosensitive regulation of splicing of a 3 intron in the period mRNA transcript. Here we demonstrate that the control of locomotor patterns by this mechanism is primarily light-dependent at low temperatures. At warmer temperatures, when it is vitally important for the fly to avoid midday desiccation, more stringent regulation of splicing is observed, requiring the light input received through the visual system during the day and the circadian clock at night. During the course of this study, we observed that a mutation in the no-receptor-potential-A P41 (norpA P41 ) gene, which encodes phospholipase-C, generated an extremely high level of 3 splicing. This cannot be explained simply by the mutation's effect on the visual pathway and suggests that norpA P41 is directly involved in thermosensitivity.period gene ͉ splicing ͉ locomotor ͉ entrainment ͉ norpA T he period (per) gene in Drosophila melanogaster plays a central role in the circadian clock, acting as a negative regulator of its own transcription (reviewed in ref. 1). A number of other positive and negative transcriptional regulators are interwoven with PER to generate the circadian cycles observed in behavior, including TIMELESS (TIM) and the basic helix-loop-helix transcription factors dCLOCK and CYCLE. In addition, kinases such as DOUBLETIME regulate PER at the protein level, thereby contributing to the timing mechanism (1).Under light͞dark cycles (LD) 12:12, D. melanogaster behavior is bimodal, with a small morning locomotor activity peak at ''lights on'' [Zeitgeber time (ZT)0] and a larger evening activity peak around ''lights off'' (ZT12). Two classic per mutations, per s and per L , alter the free-running period, and under a 24-h Zeitgeber either advance (per s ) or delay into the night phase (per L ) the evening peak, while leaving morning locomotor activity largely unaffected (2). Thus per function is particularly relevant for this latter phase of locomotor behavior, and the importance of this response has been underscored by studies in which elevated temperatures delay the evening peak under LD cycles and cooler temperatures advance it (2-4). This is clearly an adaptive response in that under hot desiccating conditions, the locomotor activity of the fly moves toward the cooler later parts of the day, generating an apparent midday ''siesta'' (4).Temperature changes regulate the splicing of an intron within the 3Ј UTR of per, which then determines the position of the evening locomotor activity peak (4). It might be predicted that the regulation of this event would be tightly controlled, particularly at high temperatures when inappropriate locomotor behavior could prove fatal. We therefore examined the levels of per mRNA splicing in a number of different mutant backgrounds that either eliminate clock function (per 01 and tim 01 ) or impair light input [cry b , gl 60j , and no-receptor-potential-A P41 (norpA P41 )], and combinations thereof.The cry b mutation is c...

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“…NO is also found at all levels of the vertebrate visual system (Vincent & Kimura, 1992;Kalamkarov et al, 1993) and it is thought to be implicated in the visual processes and in the visually guided behaviour of some insects (Elphick et al, 1996;Bacigalupo et al, 1995;Bicker & Schmachtenberg, 1997).…”

confidence: 99%

Nitric oxide synthase-dependent NADPH-diaphorase activity in the optic lobes of male and female Ceratitis capitata mutants

et al. 2009

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Nitric oxide (NO) is acknowledged as a messenger molecule in the nervous system with a pivotal role in the modulation of the chemosensory information. It has been shown to be present in the optic lobes of several insect species. In the present study, we used males and females from four different strains of the medfly Ceratitis capitata (Diptera, Tephritidae): or; or,wp (both orange eyed); w,M360 and w,Heraklion (both white eyed), as models to further clarify the involvement of NO in the mutants' visual system and differences in its activity and localization in the sexes.Comparison of the localization pattern of NO synthase (NOS), through NADPH-diaphorase (NADPHd) staining, in the optic lobes of the four strains, revealed a stronger reaction intensity in the retina and in the neuropile region lamina than in medulla and lobula. Interestingly, the intensity of NADPHd staining differs, at least in some strains, in the optic lobes of the two sexes; all the areas are generally strongly labelled in the males of the or and w,M360 strains, whereas the w,Heraklion and or,wp mutants do not show evident sexdependent NADPHd staining. Taken as a whole, our data point to NO as a likely transmitter candidate in the visual information processes in insects, with a possible correlation among NOS distribution, eye pigmentation and visual function in C. capitata males. Moreover, NO could influence behavioural differences linked to vision in the two sexes.

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Cyclic-GMP enhances light-induced excitation and induces membrane currents in Drosophila retinal photoreceptors

Cited by 35 publications

References 33 publications

Preliminary characterization of two atypical soluble guanylyl cyclases in the central and peripheral nervous system ofDrosophila melanogaster

Preliminary characterization of two atypical soluble guanylyl cyclases in the central and peripheral nervous system ofDrosophila melanogaster

Seasonal behavior in Drosophila melanogaster requires the photoreceptors, the circadian clock, and phospholipase C

Nitric oxide synthase-dependent NADPH-diaphorase activity in the optic lobes of male and female Ceratitis capitata mutants

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